Anti-pVIIIa recognizes a protein of 24 kDa in BAdV-3 infected cells (Ayalew et al., 2014 (link)). Anti-DBP recognizes a protein of 48 kDa in BAdV-3 infected cells (Zhou et al., 2001 (link)). Anti-DDX3, anti-eIF4G, anti-eIF4E, anti-eIF3, anti-PABP and fluorescence conjugated goat anti-mouse IgG-FITC (Santa Cruz Biotechnology, Inc., USA), Cy-3 conjugated goat anti-rabbit antibody (Jackson Immuno Research), Alexa Flour 680 goat anti-rabbit IgG antibody (Molecular Probes) or IRDye 800 conjugated goat anti-mouse IgG (Li-COR biosciences) and anti-HA and anti-β-actin MAb (Sigma Aldrich) were purchased.
Anti ddx3
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-DDX3 is an antibody product manufactured by Santa Cruz Biotechnology. It is designed to detect the DDX3 protein, which is a member of the DEAD-box RNA helicase family and is involved in various cellular processes. The antibody can be used for applications such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of DDX3 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti eif4e, by Santa Cruz Biotechnology (1 mentions)
Irdye 800 conjugated goat anti mouse igg, by LI COR (1 mentions)
Cy3 conjugated goat anti rabbit antibody, by Jackson ImmunoResearch (1 mentions)
Anti flag m2 mab, by Merck Group (1 mentions)
Anti gst, by Promega (1 mentions)
Anti p100 p52, by Merck Group (1 mentions)
Gst ikkα, by Merck Group (1 mentions)
Anti his, by Merck Group (1 mentions)
Anti ikkα, by Santa Cruz Biotechnology (1 mentions)
Anti irf7, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti ddx3
1
Antibody Characterization in BAdV-3 Infected Cells
2
Plasmids, Recombinant Proteins, and Antibodies
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Plasmids, recombinant proteins and antibodies DDX3 and K7 expression constructs (in pCMV-myc, pCMV-Ha or pHisParallel2) have been described in our previous studies [3, (link)4] (link). IKKα-Flag and NIK-Flag were provided by Prof. Luke O'Neill (Trinity College Dublin), and Flag-IRF7 was provided by Prof. Kate Fitzgerald (University of Massachusetts). GST (glutathione-S-transferase)-IRF7 (aa 468-503) peptides and constructs were kindly provided by Prof. John Hiscott and Dr Qiang Sun (McGill University, Montreal). Purified recombinant protein kinase GST-IKKα was purchased from Millipore and recombinant GST-NIK was purchased from Proqinase (Freiburg, Germany). The antibodies used were anti-Flag-M2 mAb (Sigma-Aldrich), anti-Myc mAb clone 9E10 (Sigma-Aldrich), anti-HA (hemagglutinin) mAb (Covance), anti-DDX3 (Santa Cruz or Bethyl Laboratories), anti-p52/p100 (Millipore), anti-IRF7 (Santa Cruz), anti-IKKα, anti-phospho-Ser176/180-IKKα, anti-phospho-Ser 866/ 870-p100, and anti-phospho-Ser471/472-IRF7 (all Cell Signaling Technology), anti-His (Sigma-Aldrich), and anti-GST (Promega). Secondary AlexaFluor488-and AlexaFluor594-coupled antibodies were purchased from Invivogen.
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