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4 cm millipede rf imaging probe

Manufactured by Agilent Technologies
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The 4 cm Millipede RF imaging probe is a compact and versatile device designed for high-resolution radio frequency (RF) imaging applications. It features a 4 cm sensor array that enables detailed RF signal measurements and analysis. The probe is optimized for use in a variety of research and testing environments, providing accurate and reliable data for various applications.

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Lab products found in correlation

Matlab 2017, by MathWorks (1 mentions) 9.4 t mri system, by Agilent Technologies (1 mentions)

3 protocols using 4 cm millipede rf imaging probe

1

High-Field MRI Imaging Protocols

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All images were acquired using a 9.4 T (400 MHz for protons) 89 mm vertical bore magnet (Varian, Walnut Creek, CA) with a 4 cm Millipede RF imaging probe with triple axis gradients (100 G/cm max). Specific imaging sequences and parameters that were used are provided in the relevant Methods sections. All acquired images were then processed in MATLAB 2017 (MathWorks, Inc., Natick, MA) as described in the relevant methods sections.
Miller H.A., Magsam A.W., Tarudji A.W., Romanova S., Weber L., Gee C.C., Madsen G.L., Bronich T.K, & Kievit F.M. (2019). Evaluating differential nanoparticle accumulation and retention kinetics in a mouse model of traumatic brain injury via Ktrans mapping with MRI. Scientific Reports, 9, 16099.
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2

Quantifying Relaxivity of Nanoparticles via MRI

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R1 relaxivity of NPs was measured using a 9.4T (400 MHz) 89 mm vertical bore magnet (Varian, Walnut Creek, CA) with a 4 cm Millipede RF imaging probe and triple axis gradients (100 G/cm max). A fast spin echo sequence was used with the following parameters: 7 repetition time (TR) values from 200–2000 ms in 300 ms increments, echo time of 32.42 ms (TE), echo train length (ETL) of 16, 25x25x3 mm3 field of view (FOV), and a 128x128 data matrix. The saturation recovery method was utilized to measure relaxation time, T1, of each NP concentration based on MR signal and the following equation:
S=S0(1−e−TRT1)
In which S is MR signal at a given voxel and S0 is the signal of the given voxel at saturation. Relaxivity, r in mM-1s-1, was then calculated as:
R1=r*C+b
Where R1 = T1-1, C is the concentration of NP and b is the y-intercept of the line.
Miller H.A., Schake M.A., Bony B.A., Curtis E.T., Gee C.C., McCue I.S., Ripperda TJ J.r., Chatzizisis Y.S., Kievit F.M, & Pedrigi R.M. (2021). Smooth muscle cells affect differential nanoparticle accumulation in disturbed blood flow-induced murine atherosclerosis. PLoS ONE, 16(12), e0260606.
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3

In vivo Assessment of Nanoparticle Accumulation

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In vivo NP assessment consisted of dynamic contrast-enhanced (DCE)-MRI using a 9.4T MRI system (Varian) equipped with a 4-cm Millipede RF imaging probe with triple-axis gradients (100 G/cm max). DCE-MRI was performed to compare the Gd-Pro-NP™ accumulation at various time-points following a CCI [21 (link), 23 (link), 35 (link)]. Briefly, mice were induced with isoflurane gas and maintained at 50 to 80 breaths per minute. Pre- and post-contrast R1 mapping were performed using the variable flip angle method with two angles, 10° and 30° [65 (link), 66 (link)]. Mice were injected with a bolus administration of 100 μL of Gd-Pro-NP™ with 0.067 mM Gd concentration via tail vein catheter followed by 100 μL PBS to flush all remaining NP solution at various time points following the CCI. A series of T1-weighted gradient echo scans with a flip angle of 30°, repetition time (TR) of 54 ms, echo time (TE) of 2.73 ms, matrix size of 128×128, 10 slices of 20×20×1 mm3 field of view (FOV), and averages (AVE) of 4 were acquired for 45 minutes following injection. Ktrans, the contrast extravasation rate constant, maps were generated using a the Patlak model and a custom least squares curve fitting routine in MATLAB from the Gd concentration and R1 maps [67 (link), 68 (link)].
Tarudji A.W., Miller H.A., Curtis E.T., Porter C.L., Madsen G.L, & Kievit F.M. (2023). SEX-BASED DIFFERENCES OF ANTIOXIDANT ENZYME NANOPARTICLE EFFECTS FOLLOWING TRAUMATIC BRAIN INJURY. Journal of controlled release : official journal of the Controlled Release Society, 355, 149-159.
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