L 21409

Manufactured by Thermo Fisher Scientific

The L-21409 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for specific core functions within laboratory settings. A detailed and unbiased description of the product's features and capabilities is not available at this time.

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Lab products found in correlation

Ab15282, by Merck Group (1 mentions) Sc 10800, by Santa Cruz Biotechnology (1 mentions) Mab3073, by Merck Group (1 mentions)

2 protocols using l 21409

Immunohistochemical Analysis of Retinal Development

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Whole embryos were immersed in a solution of 4% paraformaldehyde in PBS for 1 hour at 4°C on a rocking platform. Embryos were frozen and cut into sections 10 μm thick. Pups and adults were anesthetized and perfused transcardially with 4% PFA in 0.12 m phosphate buffer, pH 7.4. After perfusion, eyes were removed from the skull and postfixed overnight in fresh fixative. Serial frozen sections were processed for immunocytochemistry using the antibodies listed in S1 Table Lectin PNA conjugates (1/50; L-21409, Molecular Probes, OR 97402) were also used. Alexa 488- or 594-conjugated antibodies (1:200, Invitrogen) were used for secondary detection. Nuclear staining was achieved in Hoechst 33342. Fluorescent images were obtained using an Olympus confocal microscope (FV-1200-IX83).

Cases O., Joseph A., Obry A., Santin M.D., Ben-Yacoub S., Pâques M., Amsellem-Levera S., Bribian A., Simonutti M., Augustin S., Debeir T., Sahel J.A., Christ A., de Castro F., Lehéricy S., Cosette P, & Kozyraki R. (2015). Foxg1-Cre Mediated Lrp2 Inactivation in the Developing Mouse Neural Retina, Ciliary and Retinal Pigment Epithelia Models Congenital High Myopia. PLoS ONE, 10(6), e0129518.

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Retinal Neurotransmitter Receptor Localization

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The following antibody and lectins were utilized in this study: Rabbit anti-cone arrestin (cones; Millipore; AB15282; 1:1000: RRID:AB_1163387), mouse (IgG1) anti-calsenilin (type 4 cone bipolar cells; Millipore; 05–756; 1:500: RRID:AB_309969), mouse (IgG1) anti-Goa (ON bipolar cells; Millipore; MAB3073; 1:1000: RRID:AB_94671), Peanut agglutinin (PNA) (cone pedicles; Molecular Probes; L21409; 1:1000: RRID:AB_2315178), rabbit anti-PkaRIIβ (type 3b bipolar cells; BD biosciences 610625; 1:500: RRID:AB_397957), rabbit anti-PKCα (rod bipolar cells; Santa Cruz Biotechnology; sc-10800; 1:500: RRID:AB_2168560). DAPI was included in the second post-secondary antibody wash at a dilution of 1:50,000 of a 1 mg/ml stock (RRID:AB_2307445). Secondary Antibodies: Secondary antibodies were acquired from Jackson Immuno-Research (RRID:SCR_010488) and used at a concentration of 1:1000. The specificity of antibody reagents used in this study have previously been experimentally verified and described in detail (de Andrade et al., 2014 (link)).

Simmons A.B., Camerino M.J., Clemons M.R., Sukeena J.M., Bloomsburg S., Borghuis B.G, & Fuerst P.G. (2019). Increased density and age-related sharing of synapses at the cone to OFF bipolar cell synapse in the mouse retina. The Journal of comparative neurology, 528(7), 1140-1156.

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