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M3575

Manufactured by Agilent Technologies
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Sourced in Denmark

The M3575 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design, allowing for flexible configuration to meet a variety of analytical needs. The M3575 provides precise control over flow rate, pressure, and solvent mixing, ensuring reliable and reproducible results.

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Lab products found in correlation

Benchmark xt platform, by Roche (4 mentions) Caseviewer version 2, by 3DHISTECH (1 mentions) Scan 150, by 3DHISTECH (1 mentions) M7018, by Agilent Technologies (1 mentions) M7019, by Agilent Technologies (1 mentions) Ab8158, by Abcam (1 mentions) Ab52635, by Abcam (1 mentions)

7 protocols using m3575

1

Immunohistochemical Analysis of Lung Tissue

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Lung tissue samples were fixed in 10% formalin and processed for paraffin embedding. Blocks were sectioned and stained with antibodies for specific target antigens: αIL-10 (Acris, PM1121, Herford, Germany), αIL-10R (Abcam, ab197666, Cambridge, UK), αCD3 (Zytomed, RBK024, Berlin, Germany), αPD1 (Zytomed, 516-18632) and αTTF1 (Dako, M3575, Hamburg, Germany). Stained slides were scanned and digital hole slide images were performed using a digital slide scanner (Scan 150, 3D Histech Ltd, Budapest, Hungary) at the task group on digital pathology at the Institute of Pathology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Erlangen, Germany. Hole slide images were visualised by the CaseViewer (Version 2.0, 3D Histech Ltd). The immune reactive score (IRS) or the area fraction quantification was analysed with ImageJ software Version 1.46 (Bethesda, MD, USA), open source by Wayne Rasband. The detailed quantification method is described in the supplements.
Vahl J.M., Friedrich J., Mittler S., Trump S., Heim L., Kachler K., Balabko L., Fuhrich N., Geppert C.I., Trufa D.I., Sopel N., Rieker R., Sirbu H, & Finotto S. (2017). Interleukin-10-regulated tumour tolerance in non-small cell lung cancer. British Journal of Cancer, 117(11), 1644-1655.
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2

Characterization of Cell Lines

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For each newly established cell line, cultured tumor cells were centrifuged, fixed, and embedded into paraffin cell blocks according to routine cytology procedures. The cultured cells and xenografts were characterized for their morphological characteristics by routine hematoxylin and eosin stain, mucin content, and immunohistochemistry with a panel of monoclonal antibodies against cytokeratins (CK7 [M7018; DAKO {Agilent Technologies, Santa Clara, CA, USA}], CK20 [M7019; DAKO]) and the thyroid transcription factor TTF-1 (M3575; DAKO). All cell lines were subjected to DNA fingerprinting with markers STR D11S922, D11S4099, and D11S4152.
Lam D.C., Luo S.Y., Deng W., Kwan J.S., Rodriguez-Canales J., Cheung A.L., Cheng G.H., Lin C.H., Wistuba I.I., Sham P.C., Wan T.S, & Tsao S.W. (2015). Oncogenic mutation profiling in new lung cancer and mesothelioma cell lines. OncoTargets and therapy, 8, 195-209.
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3

Evaluating TTF-1 Expression by IHC

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For the evaluation of TTF-1 expression by immunohistochemistry (IHC), a mouse MoAb against TTF-1 (M3575; Dako©, Glostrup, Denmark) was used on a Benchmark XT platform (Ventana-Roche, Tucson, AZ, USA). When tumor cells presented nuclear staining, the case was considered TTF-1-positive. Data indicating the intensity, distribution and percentage of positive cells were also collected.
Giovanna S., Samantha E., Vittoria M., Francesca M., Jessica B., Luca M., Milo F, & Luciano W. (2020). Functional and clinical significance of ROR1 in lung adenocarcinoma. BMC Cancer, 20, 1085.
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4

Immunohistochemistry for Cell Markers

Cited 1 time
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Immunohistochemistry staining and analysis were carried out as previously described11 (link). Tissue slides were probed with the following antibodies: CD34 (1:800, ab8158 Abcam); TTF1 (1:1,000, M3575 Dako); CK5 (1:1,000, ab52635 Abcam).
Han M., Bushong E.A., Segawa M., Tiard A., Wong A., Brady M.R., Momcilovic M., Wolf D.M., Zhang R., Petcherski A., Madany M., Xu S., Lee J.T., Poyurovsky M.V., Olszewski K., Holloway T., Gomez A., John M.S., Dubinett S.M., Koehler C.M., Shirihai O.S., Stiles L., Lisberg A., Soatto S., Sadeghi S., Ellisman M.H, & Shackelford D.B. (2023). Spatial mapping of mitochondrial networks and bioenergetics in lung cancer. Nature, 615(7953), 712-719.
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5

Immunohistochemical Evaluation of TTF-1

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For the evaluation of TTF-1 expression by immunohistochemistry (IHC), a mouse MoAb against TTF-1 (M3575; Dako©, Glostrup, Denmark) was used on a Benchmark XT platform (Ventana-Roche, Tucson, AZ, USA). When tumor cells presented nuclear staining, the case was considered TTF-1-positive. Data indicating the intensity, distribution and percentage of positive cells were also collected.
Schiavone G., Epistolio S., Vittoria M., Francesca M., Barizzi J., Mazzucchelli L., Frattini M., & Wannesson L. (2020). Functional And Clinical Significance Of ROR1 In Lung Adenocarcinoma.
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6

Immunohistochemical Evaluation of TTF-1

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For the evaluation of TTF-1 expression by immunohistochemistry (IHC), a mouse MoAb against TTF-1 (M3575; Dako©, Glostrup, Denmark) was used on a Benchmark XT platform (Ventana-Roche, Tucson, AZ, USA). When tumor cells presented nuclear staining, the case was considered TTF-1-positive. Data indicating the intensity, distribution and percentage of positive cells were also collected.
Schiavone G., Epistolio S., Vittoria M., Francesca M., Barizzi J., Mazzucchelli L., Frattini M., & Wannesson L. (2020). Functional and Clinical Significance of ROR1 in Lung Adenocarcinoma.
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7

Immunohistochemical Evaluation of TTF-1

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the evaluation of TTF-1 expression by immunohistochemistry (IHC), a mouse MoAb against TTF-1 (M3575; Dako©, Glostrup, Denmark) was used on a Benchmark XT platform (Ventana-Roche, Tucson, AZ, USA). When tumor cells presented nuclear staining, the case was considered TTF-1-positive. Data indicating the intensity, distribution and percentage of positive cells were also collected.
Schiavone G., Epistolio S., Vittoria M., Francesca M., Barizzi J., Mazzucchelli L., Frattini M., & Wannesson L. (2021). Functional and Clinical Significance of Ror1 in Lung Adenocarcinoma.
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