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Cdna synthesis reagents

Manufactured by Promega
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The CDNA synthesis reagents from Promega are a set of enzymes, buffers, and other components designed to facilitate the reverse transcription of RNA into complementary DNA (cDNA). These reagents provide the necessary tools for the first step in various molecular biology workflows, such as gene expression analysis, quantitative PCR, and cDNA library construction.

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Lab products found in correlation

Taqman probe, by Thermo Fisher Scientific (2 mentions) Rneasy column, by Qiagen (2 mentions) Viia7 qpcr machine, by Thermo Fisher Scientific (1 mentions) Cfx qpcr machine, by Bio-Rad (1 mentions)

2 protocols using cdna synthesis reagents

1

Quantitative Gene Expression Analysis of Immune-Related Factors

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RNA was isolated from cells using RNeasy columns (Qiagen). 1 μg of RNA was reversed transcribed using cDNA synthesis reagents (Promega). qPCR was performed using the following 6-carboxyfluorescein-labeled TaqMan probes (Thermo): APOBEC3A (Hs00377444_m1), APOBEC3B (Hs00358981_m1), ACTB (Hs01060665_g1), 18S (Hs03003631_g1) IL1A (Hs00174092_m1), IL1B (Hs01555410_m1), dN-TP63 (Hs00978339_m1), total TP63 (Hs00978340_m1), RELA (Hs01042014_m1), RELB (Hs00232399), C-REL (Hs00968440_m1), IRF3 (Hs01547283_m1), STAT1 (Hs01013996_m1). qPCR was performed using an AB Applied Biosystems ViiA7 qPCR machine.
Garcia N.M., Becerra J.N., McKinney B.J., DiMarco A.V., Wu F., Fitzgibbon M, & Alvarez J.V. (2023). APOBEC3 activity promotes the survival and evolution of drug-tolerant persister cells during acquired resistance to EGFR inhibitors in lung cancer. bioRxiv.
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2

Quantitative PCR Analysis of Tumor-Related Genes

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RNA was isolated from tumors and cells using RNeasy columns (Qiagen, Hilden, Germany). 1 μg of RNA was reversed transcribed using cDNA synthesis reagents (Promega, Madison, WI). qPCR was performed using 6-carboxyfluorescein labeled TaqMan probes (Thermo, Waltham, MA): CCL5 (Mm01302427_m1), CXCL1 (Mm04207460_m1), CXCL2 (Mm00436450_m1), CXCL5 (Mm00436451_g1), CCL2 (Mm00441242_m1), Actin (Mm02619580_g1), ASPN (Mm00445945_m1), PCOLCE (Mm00476608_m1), COL5A1 (Mm00489299_m1), COL24A1 (Mm01323744_m1), and read on a Bio-Rad (Hercules, CA) CFX qPCR machine.
Walens A., DiMarco A.V., Lupo R., Kroger B.R., Damrauer J.S, & Alvarez J.V. (2019). CCL5 promotes breast cancer recurrence through macrophage recruitment in residual tumors. eLife, 8, e43653.
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