Anti hif 1α nb100 479

Manufactured by Novus Biologicals

Anti-HIF-1α (NB100-479) is a lab equipment product produced by Novus Biologicals. It is an antibody that specifically binds to the HIF-1α protein. HIF-1α is a transcription factor that plays a crucial role in the cellular response to hypoxia.

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Lab products found in correlation

Anti β actin a5441, by Merck Group (3 mentions) Anti hif 2α nb100 122, by Novus Biologicals (2 mentions) Hybond c extra, by Cytiva (2 mentions) Hiperfect transfection reagent, by Qiagen (2 mentions) Anti erk sc94, by Santa Cruz Biotechnology (1 mentions) Anti nedd8 ab81264, by Abcam (1 mentions) Anti ho 1 ma1 112, by Thermo Fisher Scientific (1 mentions) Anti α tubulin t9026, by Merck Group (1 mentions) Anti p akt1 2 3 sc 7985 r, by Santa Cruz Biotechnology (1 mentions) Pcmv6 entry vector, by OriGene (1 mentions) Anti phospho erk, by Cell Signaling Technology (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Nucleobond xtra midi, by Macherey-Nagel (1 mentions)

3 protocols using anti hif 1α nb100 479

Immunoblotting for HIF-1α and HIF-2α

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Enhanced chemiluminescence (ECL) reagents, nitrocellulose membranes (Hybond-C extra), and secondary Cy3-conjugated antibodies were from Amersham Pharmacia Biotech Inc. (Piscataway, NJ, USA). The following antibodies were used: anti-HIF-1α (NB100-479) and anti-HIF-2α (NB100-122) from Novus Biologicals; anti SB3 from Xeptagen; anti-total UbQ (sc-8017), anti-LaminA (sc-20680), anti-p53 (sc-6243), anti-p21 (sc-817), anti-gliceraldehyde-3-phosphate dehydrogenase (GAPDH) (sc-20357), and anti-ERK (sc-94) from Santa Cruz Biotechnology; anti-NEDD8 (ab81264) from Abcam; anti-NAE-1 (AP13067c) from Abgent; anti-UBE-1 (BML-PW8395-00251) from ENZO Life Sciences; anti-HO-1 (MA1-112) from Invitrogen; anti-Ub-48 (05-137) from Millipore; anti-P-ERK (#4696) from Cell Signaling Technology; anti-α-tubulin (T9026) and anti-β-actin (A5441) from Sigma Aldrich. DMOG (Dimethyloxalylglycine) and all other reagents of analytical grade were from Sigma Aldrich. HiPerfect Transfection Reagent was from Qiagen. Lactate Colorimetric/Fluorometric assay kit was from Biovision Incorporation.

Cannito S., Foglia B., Villano G., Turato C., C Delgado T., Morello E., Pin F., Novo E., Napione L., Quarta S., Ruvoletto M., Fasolato S., Zanus G., Colombatto S., Lopitz-Otsoa F., Fernández-Ramos D., Bussolino F., Sutti S., Albano E., Martínez-Chantar M.L., Pontisso P, & Parola M. (2019). SerpinB3 Differently Up-Regulates Hypoxia Inducible Factors-1α and -2α in Hepatocellular Carcinoma: Mechanisms Revealing Novel Potential Therapeutic Targets. Cancers, 11(12), 1933.

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Antibody Immunoblotting Protocol

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Enhanced chemiluminescence reagents and nitrocellulose membranes (Hybond-C extra) were from Amersham Pharmacia Biotech, Inc (Piscataway, NJ). The following antibodies were used: anti–HIF-1α (NB100-479) and anti–HIF-2α (NB100-122) from Novus Biologicals (Cambridge, UK); anti-CD105 (PA5-12511), anti-PCNA (PA5-27214), and anti-SB3 (PA5-30164) from ThermoFisher Scientific (Rockford, IL); anti–α-SMA (M0851) was from DAKO (Agilent, St Clara, CA); anti-SB3 (GTX32866) was from GeneTex (Irvine, CA); anti-F4/80 (14-4801-82) was from eBioscience (Affymetrix, St Clara, CA); anti-YAP (sc-15407), anti-c-MYC (sc-788), anti-SB3 (sc21767), anti-p53 (sc-6243), anti-p21 (sc-817), anti-ERK (sc-94), anti-JNK (sc-571), antivinculin (sc-73614), anti–p-Akt1/2/3 (sc-7985-R), and anti-Akt1/2/3 (sc-8312) were from Santa Cruz Biotechnology (Dallas, TX); anti-phospho-ERK (extracellular-regulated kinase, [4696]) and anti–anti-phospho-JNK (c-Jun aminoterminal kinase [9255]) were from Cell Signaling Technology (Danvers, MA); and anti–β-actin (A5441) was from Sigma Aldrich (St. Louis, MO). HiPerfect Transfection reagent was from Qiagen (Hilden, Germany), Lipofectamine 2000 was from Invitrogen-Life Technologies (Carlsbad, CA), plasmid DNA purification NucleoBond XtraMIDI was from Macherey-Nagel (Allentown, PA), and pCMV6-entry vectors were from Origene (Rockville, MD).

Foglia B., Sutti S., Cannito S., Rosso C., Maggiora M., Autelli R., Novo E., Bocca C., Villano G., Ramavath N.N., Younes R., Tusa I., Rovida E., Pontisso P., Bugianesi E., Albano E, & Parola M. (2021). Hepatocyte-Specific Deletion of HIF2α Prevents NASH-Related Liver Carcinogenesis by Decreasing Cancer Cell Proliferation. Cellular and Molecular Gastroenterology and Hepatology, 13(2), 459-482.

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Luciferase Reporter Assay for Oxidative Stress

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The luciferase reporter plasmids containing ARE(+)/HRE(+),
ARE(−)/HRE(+), ARE(−)/HRE(−), and TATA box of the human
ferritin H enhancer/promoter regions fused to a firefly luciferase gene were described
previously (22 (link)). 5′-UTR sequence containing
the IRE of ferritin H mRNA was described (27 (link)), and
the IRE-luciferase plasmid was constructed by cloning the IRE into ferritin H TATA box
luciferase reporter. Antibodies utilized in Western blotting were purchased from the
following companies: anti-HIF-1α (NB100-479), Novus Biologicals; anti-lamin B
(Ab-1), Oncogene; anti-ferritin H (SC-25617), anti-NQO1 (SC-32793), anti-thioredoxin
(SC-20146), anti-thioredoxin reductase 1 (SC-28321), anti-glutamate-cysteine ligase,
modifier subunit (SC-22754), anti-IRP2 (SC-33682), anti-Nrf2 (SC-13032), all from Santa
Cruz Biotechnology; anti-IRP1 (ab126595), Abcam; and anti-β-actin (A5441),
Sigma-Aldrich.

Huang B.W., Miyazawa M, & Tsuji Y. (2014). Distinct Regulatory Mechanisms of the Human Ferritin Gene by Hypoxia and Hypoxia Mimetic Cobalt Chloride at the Transcriptional and Post-transcriptional Levels. Cellular signalling, 26(12), 2702-2709.

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