5,5’-Dithiobis-(2-nitrobenzoic acid) (DTNB) and β-nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium salt (NADPH) were purchased from Roche (Basel, Switzerland). RPMI 1640 and fetal bovine serum were from Gibco (Invitrogen Corporation, Carlsbad, USA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) and PZQ were sourced from Sigma-Aldrich Inc. (Saint Louis, USA). The Nitric Oxide Assay Kit and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (carboxy-PTIO) came from Beyotime Biotechnology Corporation (Shanghai, China). Ethylenediaminetetraacetic acid (EDTA) and 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) were purchased from Sangon Biotech Co. Ltd. (Shanghai, China), and artemisinin (AS) was purchased from Zelang Medical Technology Co. Ltd. (Nanjing, China).
Ethylenediaminetetraacetic acid edta
Manufactured by Sangon
Sourced in China
Ethylenediaminetetraacetic acid (EDTA) is a chemical compound commonly used as a chelating agent in laboratory settings. It has the ability to form stable complexes with various metal ions, including calcium, iron, and magnesium. EDTA is a white, crystalline solid that is soluble in water and other polar solvents. It is widely used in analytical and purification procedures to remove or control the concentration of metal ions in solutions.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt dimethyl sulfoxide dmso, by Merck Group (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
2 4 2 hydroxyethyl 1 piperazinyl ethanesulfonic acid hepes, by Sangon (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Z vad fmk, by BD (1 mentions)
B ap15, by R&D Systems (1 mentions)
Boc lrr amc, by R&D Systems (1 mentions)
Z lle amc, by R&D Systems (1 mentions)
Suc llvy amc, by R&D Systems (1 mentions)
Annexin 5, by Merck Group (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Imidazole, by Sangon (1 mentions)
Polyacrylamide, by Sangon (1 mentions)
Sodium acetate, by Sangon (1 mentions)
Magnesium chloride, by Sangon (1 mentions)
Ethanol, by Sangon (1 mentions)
Ni nta agarose, by GE Healthcare (1 mentions)
Sephadex g 25, by GE Healthcare (1 mentions)
5 fluorodeoxyuridine, by Thermo Fisher Scientific (1 mentions)
4 protocols using ethylenediaminetetraacetic acid edta
1
DTNB and NADPH Cytotoxicity Assay
2
Apoptosis and Proteasome Inhibition Assay
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PtPT was synthesized in our laboratory and stored as a 20 mM stock solution in dimethyl sulfoxide (DMSO) at −20 °C. Annexin V, propidium iodide (PI) and rhodamine-123 were purchased from Sigma-Aldrich (St. Louis, MO, USA). The proteasome inhibitor, bortezomib (PS341), was purchased from BD Biosciences (San Jose, CA, USA). b-AP15, Suc-LLVY-AMC, Z-LLE-AMC, Boc-LRR-AMC were provided from Boston Biochem (Cambridge, MA, USA). The pan-caspase inhibitor, z-VAD-fmk, was purchased from BD Biosciences. These reagents were dissolved in DMSO as a stock solution, and stored at −20 °C. In all experiments, the final concentration of DMSO did not exceed 0.1%. The metal chelator, ethylenediamine tetraaceticacid (EDTA), was obtained from Sangon Biotech (Shanghai, China). A stored solution (100 mM) was prepared by dissolving EDTA in H2O and kept at −20 °C.
3
Reagents for Biomolecular Modification
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5-Fluorodeoxyuridine was purchased from Alfa Aesar (Shanghai, China). 4,4ʹ-Dimethoxytrityl Chloride (DMT-Cl, 97%) N,N-Diisopropylethylamine (DIPEA, 97%) and 2-Cyanoethyl N,N,N’,N’-tetraisopropylphosphordiamidite (95%) were obtained from Macklin Inc. (Shanghai, China). N-(ε-malemidocaproyloxy) succinimide ester (EMCS) was purchased from Aladdin (Shanghai, China). Ni-NTA agarose, and Sephadex G-25 were obtained from GE Healthcare (Piscataway, USA). Imidazole, sodium chloride, sodium acetate, polyacrylamide, tris base, acetic acid, ethylenediaminetetraacetic acid (EDTA), magnesium chloride, ethanol, and DAPI were obtained from Sangon Biotech (Shanghai) Co., Ltd (China). Amicon ultracentrifugal filters were purchased from Merck Millipore Ltd (Darmstadt, Germany). RPMI 1640 medium, DMEM medium, MEBM medium, trypsin, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), antibiotic-antimycotic (100×) and fetal bovine serum (FBS) were purchased from Wisent Biotechnology (Nanjing) Co. Ltd (China). All chemicals were used without further purification.
4
Antioxidant Capacity Evaluation Protocol
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Folin–Ciocalteu reagent, gallic acid, daidzein, glycitin, L-ascorbic acid (ASA), 2,2’-azobis-amidinopropane (ABAP), Trolox and Fluorescein disodium salt were purchased from Sigma Aldrich (St. Louis, Mo, USA). Acetone, sodium hydroxide (NaOH), methanol, ethylenediamine tetra-acetic acid (EDTA), Sodium carbonate (Na2CO3), potassium dihydrogen phosphate (KH2PO4) and dipotassium hydrogen phosphate (K2HPO4) were purchased from Sangon Biotech Co. Ltd. (Shanghai, China). Acetonitrile and methanol (HPLC grade) were purchased from ANPEL Scientific Instrument Co. (Shanghai, China). Other reagents used were analytical grade.
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