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Mouse il 6 elisas

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The Mouse IL-6 ELISAs are quantitative sandwich enzyme-linked immunosorbent assays (ELISAs) designed for the measurement of mouse interleukin-6 (IL-6) levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Tnfα elisas, by R&D Systems (1 mentions) Mouse tnf α elisas, by R&D Systems (1 mentions)

Close spelling variants of this product

ELISA (Mouse IL-6; IL-6 ELISAs Mouse IL-6 ELISAs

3 protocols using mouse il 6 elisas

1

Measuring Mouse IL-6 and IL-1β

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Mouse IL-6 ELISAs and mouse IL-1β ELISAs (BD, Heidelberg, Germany) were performed according to the manufacturer’s instructions. IL-6 was measured in supernatants. IL-1β was measured from total cell lysates (TCL) and supernatants (SN). Levels of cytokines varied between experiments due to genetic background or age of the cells. Qualitative differences or similarities between WT and mutant cells, however, were consistent throughout the study.
Kuhny M., Hochdörfer T., Ayata C.K., Idzko M, & Huber M. (2014). CD39 is a negative regulator of P2X7-mediated inflammatory cell death in mast cells. Cell Communication and Signaling : CCS, 12, 40.
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2

Mouse Cytokine ELISA Assays

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Mouse IL-6 ELISAs (BD Pharmingen, Heidelberg, Germany) and TNF-α ELISAs (R&D Systems, Wiesbaden- Nordenstadt, Germany) were carried out according to the manufacturer’s instructions. Levels of cytokine production were measured in supernatants.
Poplutz M., Levikova M., Lüscher-Firzlaff J., Lesina M., Algül H., Lüscher B, & Huber M. (2017). Endotoxin tolerance in mast cells, its consequences for IgE-mediated signalling, and the effects of BCL3 deficiency. Scientific Reports, 7, 4534.
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3

Quantification of Mouse Cytokines

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Mouse IL-6 ELISAs (BD Pharmingen, Heidelberg, Germany) and mouse TNF-α ELISAs (R&D Systems, Wiesbaden-Nordenstadt, Germany) were carried out according to the manufacturer’s instructions. Concentrations of cytokines varied between experiments/cell cultures. Qualitative differences or similarities, however, were consistent throughout the study.
Nunes de Miranda S.M., Wilhelm T., Huber M, & Zorn C.N. (2016). Differential Lyn-dependence of the SHIP1-deficient mast cell phenotype. Cell Communication and Signaling : CCS, 14, 12.
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