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03 gp62 c

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The 03-GP62-C is a laboratory equipment product designed for general research purposes. It serves as a core function in various laboratory applications. Further details on the specific intended use of this product are not available.

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Lab products found in correlation

β actin antibody a1978, by Merck Group (2 mentions) Ha antibody prb 101p, by Fortrea (2 mentions) Bafilomycin a1 b 1080, by LC Laboratories (2 mentions) Rapamycin r0395, by Merck Group (2 mentions) Hif2α antibody nb100 122, by Novus Biologicals (2 mentions) Atg7 antibody 8558, by Cell Signaling Technology (2 mentions) Beclin 1 antibody b6186, by Merck Group (2 mentions) Gfp antibody sc 8334, by Santa Cruz Biotechnology (2 mentions) Sc 28359, by Santa Cruz Biotechnology (2 mentions) Lamp1 antibody, by BD (2 mentions) P53 antibody sc 126, by Santa Cruz Biotechnology (2 mentions) β actin, by Merck Group (1 mentions) Sc 365062, by Merck Group (1 mentions)

3 protocols using 03 gp62 c

1

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.
Liu X.D., Yao J., Tripathi D.N., Ding Z., Xu Y., Sun M., Zhang J., Bai S., German P., Hoang A., Zhou L., Jonasch D., Zhang X., Conti C.J., Efstathiou E., Tannir N.M., Eissa N.T., Mills G.B., Walker C.L, & Jonasch E. (2014). Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene, 34(19), 2450-2460.
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2

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.
Liu X.D., Yao J., Tripathi D.N., Ding Z., Xu Y., Sun M., Zhang J., Bai S., German P., Hoang A., Zhou L., Jonasch D., Zhang X., Conti C.J., Efstathiou E., Tannir N.M., Eissa N.T., Mills G.B., Walker C.L, & Jonasch E. (2014). Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene, 34(19), 2450-2460.
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3

Protein Extraction and Immunoblotting

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Different tissues were ground in a Cryomill machine (Retsch) and then total protein extracts were isolated by Tris lysis buffer (1 mol/L Tris HCl, 1 mol/L NaCl, 0.1 mol/L EDTA, 10% NP40). Separated proteins were probed with antibodies against ATG7 (1:2000; Sigma A2856), LC3 (1:1500; Novus Biologicals NB600-1384), p62 (1:2000; American Research Products 03-GP62-C), GAPDH (1:1000; Santa Cruz Biotechnology sc-365062), and β-actin (1:5000; Sigma A1978).
Yang Y., Karsli-Uzunbas G., Poillet-Perez L., Sawant A., Hu Z.S., Zhao Y., Moore D., Hu W, & White E. (2020). Autophagy promotes mammalian survival by suppressing oxidative stress and p53. Genes & Development, 34(9-10), 688-700.
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