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Anti cd11b percp cyanine5.5 clone m1 70

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Anti-CD11b-PerCP-Cyanine5.5 (clone M1/70) is a monoclonal antibody conjugated with PerCP-Cyanine5.5 dye. It is designed to detect and identify CD11b, a cell surface glycoprotein involved in the adhesion and migration of cells.

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Lab products found in correlation

Facscantotm cytometer, by BD (1 mentions) Flowjotm software, by Tree Star (1 mentions) Anti f4 80 apc clone bm8, by Thermo Fisher Scientific (1 mentions) Facscanto, by BD (1 mentions)

2 protocols using anti cd11b percp cyanine5.5 clone m1 70

1

Phenotypic Analysis of Murine EVs

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For tracking of labeled EVs and phenotypical analysis of murine organs, femurs were flushed and single-cell suspensions were filtered through a 40-μm strainer. Cells were washed in PBS with 1% BSA and incubated with anti-CD11b-PerCP-Cyanine5.5 (clone M1/70, 1:100, BD Biosciences – 561114) at predetermined saturating concentrations. PKH67-labeled EV-positive cells were detected using blue laser excitation and 488 nm emission. Data for 1,000,000 cells was acquired on a BD FACS CantoTM cytometer with Diva software (BD) and was analyzed using FlowJoTM software (TreeStar).
Maia J., Otake A.H., Poças J., Carvalho A.S., Beck H.C., Magalhães A., Matthiesen R., Strano Moraes M.C, & Costa-Silva B. (2020). Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles. Frontiers in Cell and Developmental Biology, 8, 592518.
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2

Exosome Tracking and Organ Analysis

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For labeled exosome tracking and phenotypic analysis of murine organs, femurs were flushed and livers were mechanically dissociated, and single cell suspensions were filtered through a 40μm strainer. Cells were washed in PBS with 1% BSA and incubated with anti-CD11b-PerCP-Cyanine5.5 (clone M1/70, 1:100, BD Biosciences) and anti-F4/80-APC (clone BM8, 1:100, eBioscience) antibodies at pre-determined saturating concentrations. PKH67-labeled exosome positive cells were detected using blue laser excitation and 488nm emission. Data for 1,000,000 cells was acquired on a BD FACS Canto™ cytometer with Diva software (BD) and was analyzed using FlowJo™ software (TreeStar).
Costa-Silva B., Aiello N.M., Ocean A.J., Singh S., Zhang H., Thakur B.K., Becker A., Hoshino A., Mark M.T., Molina H., Xiang J., Zhang T., Theilen T.M., García-Santos G., Williams C., Ararso Y., Huang Y., Rodrigues G., Shen T.L., Labori K.J., Lothe I.M., Kure E.H., Hernandez J., Doussot A., Ebbesen S.H., Grandgenett P.M., Hollingsworth M.A., Jain M., Mallya K., Batra S.K., Jarnagin W.R., Schwartz R.E., Matei I., Peinado H., Stanger B.Z., Bromberg J, & Lyden D.C. (2015). Pancreatic cancer exosomes initiate pre-metastatic niche formation in the liver. Nature cell biology, 17(6), 816-826.
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