The human colorectal cancer cell lines HCT116 and LoVo were obtained from the American Type Culture Collection (ATCC; USA). HCT116 and LoVo cells were cultured with RPMI Medium 1640 basic (1X) (Gibco, Thermo Fisher Scientific, Suzhou, China) supplemented with 10% fetal bovine serum (FBS; Gibco, Life technologies, New Zealand) and 1% penicillin streptomycin (PS; Gibco, Life technologies, USA). The appropriate temperature (37°C), CO2 (5%), and humidity conditions are also indispensable for the routine maintenance of the cells.
Rpmi medium 1640 basic 1x
Manufactured by Thermo Fisher Scientific
Sourced in China
RPMI Medium 1640 basic (1X) is a widely used cell culture medium that provides a balanced salt solution and essential nutrients to support the growth and maintenance of various cell types in vitro. It is a complete and chemically defined medium that is commonly used in a range of applications, including cell culture, tissue engineering, and biomedical research.
Automatically generated - may contain errors
4 protocols using rpmi medium 1640 basic 1x
1
Culturing Colorectal Cancer Cell Lines
2
Evaluating Platinum-Resistant Ovarian Cancer Cells
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Two types of EOC cells,
A2780 cells and A2780-CisR cells, were
studied in this work. A2780 cells were from the ATCC (American Type
Culture Collection) and preserved in the Obstetrics and Gynecology
Laboratory of Peking University People’s Hospital. The drug-resistant
characteristic of A2780-CisR cells was obtained by exposing A2780
cells to different concentrations of platin for 10 months. A2780 and
A2780-CisR cells were cultured and grown in 1640 (RPMI Medium 1640
basic 1X, GIBCO, China) supplemented withl -glutamine, 15
mM HEPES, and 10% fetal bovine serum (Gibco 10099-141, Australia).
Cells were seeded in a 100 mm glass Wilson dish at a density of 13.7
× 106 cells for 24 h with 5% CO2 and 37
°C. After the treatment, the cells were collected from wells
using trypsin digestion, washed in phosphate-buffered saline (PBS)
to dilute to half the concentration (about 5 × 106), and then suspended in 500 μL of 1x binding buffer (10 mM
HEPES, 140 mM NaCl, 2.5 mM CaCl2, pH 7.4) with 10 μL
of annexin conjugate and propidium iodide (PI).
A2780 cells and A2780-CisR cells, were
studied in this work. A2780 cells were from the ATCC (American Type
Culture Collection) and preserved in the Obstetrics and Gynecology
Laboratory of Peking University People’s Hospital. The drug-resistant
characteristic of A2780-CisR cells was obtained by exposing A2780
cells to different concentrations of platin for 10 months. A2780 and
A2780-CisR cells were cultured and grown in 1640 (RPMI Medium 1640
basic 1X, GIBCO, China) supplemented with
mM HEPES, and 10% fetal bovine serum (Gibco 10099-141, Australia).
Cells were seeded in a 100 mm glass Wilson dish at a density of 13.7
× 106 cells for 24 h with 5% CO2 and 37
°C. After the treatment, the cells were collected from wells
using trypsin digestion, washed in phosphate-buffered saline (PBS)
to dilute to half the concentration (about 5 × 106), and then suspended in 500 μL of 1x binding buffer (10 mM
HEPES, 140 mM NaCl, 2.5 mM CaCl2, pH 7.4) with 10 μL
of annexin conjugate and propidium iodide (PI).
3
Cell Line Cultivation for Cancer Research
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The lung cancer cell lines A549 and H1299 were grown in DMEM/ high glucose medium (HyClone) with 10% Fetal Bovine Serum at 37 °C under an atmosphere containing 5% CO2. The normal epithelial cell line HBE was grown in RPMI Medium 1640 basic (1x) (Gibco by life technologies) with 10% Fetal Bovine Serum at 37 °C under an atmosphere containing 5% CO2.
4
Cultivation of Human Ovarian Granulosa Cells
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COV434 (human ovarian granulosa cells) was obtained from GuangZhouJennio Biotech Co., Ltd, China. The cells were cultured in RPMI Medium 1640 basic (1X) (Gibco) supplemented with 10% fetal bovine serum (Gaithersburg, MD, USA) in a humidified incubator with 5% CO2 at 37 °C.
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