Brij l23

Manufactured by Merck Group

Sourced in United States

Brij L23 is a nonionic surfactant belonging to the polyoxyethylene alkyl ether family. It is commonly used as a laboratory reagent and emulsifier.

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Lab products found in correlation

Paraformaldehyde (pfa), by Merck Group (5 mentions) 3 aminopropyltriethoxysilane, by Merck Group (4 mentions) Trizma base, by Merck Group (3 mentions) Bovine serum albumin (bsa), by Merck Group (3 mentions) Tyramine hydrochloride, by Merck Group (2 mentions) Sodium hydroxide (naoh), by Merck Group (2 mentions) Brij 58, by Merck Group (2 mentions) Pluronic f 127, by Merck Group (2 mentions) Brij o20, by Merck Group (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Spectrophotometry, by Molecular Devices (1 mentions) Synergy h1, by Agilent Technologies (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions) Tergitol 15 s 9, by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Nystatin, by Fujifilm (1 mentions) Brij 97, by Merck Group (1 mentions) O pdm, by Merck Group (1 mentions)

20 protocols using brij l23

Quantifying NCK2 SH3 Domain Interactions

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Increasing amounts of NCK2 full-length recombinant proteins in GST buffer (final concentrations of 0–150 μM for CD3ε and 0–175 μM for PAK1) were used for binding assays with a constant amount of fluorescein isothiocyanate-conjugated peptides (resuspended in 17 mM Tris-HCl pH 8, 100 mM NaCl and 0.5% Brij L23 (Millipore Sigma), peptides final concentration of 40 nM). Cd3ε (RGQNKERPPPVPNPDY) and PAK1 (DIQDKPPAPPMRNTST) peptides were used as NCK2 SH3-1 and NCK2 SH3-2 ligands, respectively. Binding assays were performed in the FP buffer (17 mM Tris-HCl pH 8 and 100 mM NaCl) and fluorescence polarization was measured on a Synergy H1 multimode plate reader (Bio Tek) at 535 nm with 485 nm excitation. Calculation of the dissociation constants was performed with a one-site total binding model and nonlinear regression in GraphPad Prism version 7.

Dionne U., Bourgault É., Dubé A.K., Bradley D., Chartier F.J., Dandage R., Dibyachintan S., Després P.C., Gish G.D., Pham N.T., Létourneau M., Lambert J.P., Doucet N., Bisson N, & Landry C.R. (2021). Protein context shapes the specificity of SH3 domain-mediated interactions in vivo. Nature Communications, 12, 1597.

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Quantifying Lung Wet Weight and EVLW

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Lung wet weight and extravascular lung water (EVLW) were quantified in the same experiment. Body weight was recorded in anesthetized mice at the time of lung excision in untreated mice or at the time of IAV instillation in coinfected mice. We used our established methods (27 (link)) to exsanguinate anesthetized mice by cardiac puncture, then excised and weighed the lungs. Blood-free EVLW content was quantified by the method of Selinger and colleagues (80 (link)), which we have used previously (27 (link)). Lungs were cut with scissors, then processed using a handheld tissue homogenizer. Hemoglobin content was determined by spectrophotometry (Molecular Devices) using hemoglobin standards and a solution of Drabkin’s reagent and Brij L23 (MilliporeSigma). Homogenate, supernatant, and blood samples were dried for 24 hours in a vacuum oven at 57°C and –5 mmHg. Total EVLW content was normalized to body weight to account for increases of lung dry weight due to extravasated protein (81 (link)).

Tang S., De Jesus A.C., Chavez D., Suthakaran S., Moore S.K., Suthakaran K., Homami S., Rathnasinghe R., May A.J., Schotsaert M., Britto C.J., Bhattacharya J, & Hook J.L. (2023). Rescue of alveolar wall liquid secretion blocks fatal lung injury due to influenza-staphylococcal coinfection. The Journal of Clinical Investigation, 133(19), e163402.

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Quantitative Protein-Peptide Interactions

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For NCK1, protein-substrate binding was performed using a SH3 domain concentration of 0–500 μM and a constant FITC-conjugated Cd3ε peptide (WT: RGQNKERPPPVPNPDY and mutant: RGQNKERAAAVANADY) at a concentration of 10 nM. For GRB2 SH3-1 and SH3-2, peptides from SOS1 (EVPVPPPVPPRRRPES) and GAB2 (IQPPPVNRLKPDRKA) were used at 10 nM and titration was performed with SH3 concentrations of 0–50 μM and 0–15 μM, respectively. For PDGFRβ kinase assay with phosphorylated GRB2 SH3-1 WT and Y52F, SH3 domains were incubated with 4 μg of PDGFRβ overnight at 37 °C and 1 mM ATP in kinase buffer prior to titration. Reactions were assembled in 15 mM Tris-HCl pH 8.0, 100 mM NaCl and 0.1% Brij L23 (Sigma). Fluorescence polarization was measured at 535 nm with 485 nm excitation on a Synergy H1 multi-mode plate reader (BioTek). Dissociation constants were calculated in GraphPad Prism (version 7) using nonlinear regression and a one-site total binding model incorporating non-specific binding to fit the data.

Dionne U., Chartier F.J., de los Santos Y.L., Lavoie N., Bernard D.N., Banerjee S.L., Otis F., Jacquet K., Tremblay M.G., Jain M., Bourassa S., Gish G.D., Gagné J.P., Poirier G.G., Laprise P., Voyer N., Landry C.R., Doucet N, & Bisson N. (2018). Direct phosphorylation of Src Homology (SH) 3 domains by tyrosine kinase receptors disassembles ligand-induced signalling networks. Molecular cell, 70(6), 995-1007.e11.

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Immunofluorescence Staining Reagents

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Paraformaldehyde (PFA) was purchased from Merck (Darmstadt, Germany). Trizma base, bovine serum albumin (BSA), 3-aminopropyl-triethoxysilane, Triton X-100, Tyramine hydrochloride and Brij L23 were purchased from Sigma Chemical Co. (St Louis, MO, USA). Clariom S microarray chip for mouse, click-iT Cell Reaction Buffer Kit, FITC- and rhodamine-succinimidyl esters and Lipofectamine RNA iMAX were purchased from Invitrogen (California, USA). 5-Ethynyl-2'-deoxyuridine (EdU) was purchased from TCI chemicals (Tokyo, Japan). All other reagents used in this study were purchased from Fujifilm Wako Pure Chemicals (Osaka, Japan).

Yano K., Choijookhuu N., Ikenoue M., F.i.d.y.a., Fukaya T., Sato K., Lee D., Taniguchi N., Chosa E., Nanashima A, & Hishikawa Y. (2022). Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration. Scientific Reports, 12, 11962.

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Optimization of DBS Eluents for SARS-CoV-2

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To determine the appropriate eluent for DBS samples, six candidate buffers were assessed: phosphate buffered saline (PBS), PBS + tween 20 at 5% weight / volume (W/V), deionized water, Brij L23 (Sigma Aldrich), Tergitol 15-S-9 (Sigma Aldrich), and ECOSURF EH (Sigma Aldrich). Undiluted (Neat) buffer, 300ul buffer eluates of blank Whatman card punches, and 300ul buffer eluates from 5 punches of Whatman cards spotted with 60 ul of SARS-CoV-2 negative whole blood were analyzed in triplicate to assess overall background by comparing the relative light units (RLU) from each eluent.

Omosule C.L., Conklin J., Seck S., Howell R., Hock K.G., Ballman C., Freeman J., Du Toit L., Dubberke E, & Farnsworth C.W. (2022). Qualitative and quantitative detection of SARS-CoV-2 antibodies from dried blood spots. Clinical Biochemistry, 117, 16-22.

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Synthesis of Thermoresponsive PAMPS Hydrogels

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The polymer poly(2-acrylamido-2-methyl-1-propanesulfonic acid) (in solution, 15 wt.% in water) (PAMPS), the monomers 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), N-isopropylacrylamide (NIPAAm), the crosslinker N,N′-methylenebis(acrylamide) (MBA), the initiator system consisting of ammonium persulfate (APS) and N,N,N′,N′-tetramethylethylenediamine (TEMED), the surfactant Brij L23, sodium hydroxide and sodium chloride were bought from Sigma Aldrich (Munich, Germany) and were used as received.

Franke D, & Gerlach G. (2020). Swelling Studies of Porous and Nonporous Semi-IPN Hydrogels for Sensor and Actuator Applications. Micromachines, 11(4), 425.

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Chemical Crosslinking Reagents Evaluation

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The following reagents were used. Cys-specific chemical crosslinkers: BMH and TMEA (Thermo Scientific), o-PDM and p-PDM (Sigma) and Bis-MTS (methanethiosulfonate) reagents (M1M through M17M) (Toronto Research Chemicals). Detergents: Brij L23, Brij 97 and Brij 99 (Sigma), Triton-X-100, NP-40 and Tween-20 (MP Biochemical) and Digitonin (Sigma and Calbiochem). o-phenanthroline (Wako). Polyene antibiotics: nystatin (Wako) and natamycin (Fluka). Other chemicals were purchased from Sigma, Wako Pure Chemical, Nacalai Tesque and BD.

Tatebayashi K., Yamamoto K., Nagoya M., Takayama T., Nishimura A., Sakurai M., Momma T, & Saito H. (2015). Osmosensing and scaffolding functions of the oligomeric four-transmembrane domain osmosensor Sho1. Nature Communications, 6, 6975.

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Cys-Specific Chemical Crosslinking Reagents

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The following reagents were used. Cys-specific chemical crosslinker: BMH (Thermo Scientific). Detergents: Brij L23 (Sigma), Triton X-100, Tween-20 (MP Biochemical), and Digitonin (Calbiochem). Other chemicals were purchased from Sigma, Wako Pure Chemical, Nacalai Tesque, and BD.

Takayama T., Yamamoto K., Saito H, & Tatebayashi K. (2019). Interaction between the transmembrane domains of Sho1 and Opy2 enhances the signaling efficiency of the Hog1 MAP kinase cascade in Saccharomyces cerevisiae. PLoS ONE, 14(1), e0211380.

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Analytical Characterization of Biochemical Compounds

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All chemicals used were of analytical grade. Thiobarbituric acid (TBA), trichloroacetic acid (TCA), 1,1,3,3-tetramethoxypropane, Brij-L23, tetramethylmurexide, and L-β-(3,4 dihydroxyphenyl) alanine (L-DOPA) were procured from Sigma Aldrich (St. Louis, MO, USA). Potassium carbonate and copper (II) sulfate were purchased from Thermo Fisher Scientific (Auckland, New Zealand). The microbial media (eosin methylene blue agar (EMB), Pseudomonas isolation agar (PIA), plate count agar (PCA), triple sugar iron agar, and Mueller–Hinton broth (MHB) were acquired from Himedia (Mumbai, India).

Ahmad A.S., Sae-leaw T., Zhang B., Singh P., Kim J.T, & Benjakul S. (2023). Impact of Ethanolic Thai Indigenous Leaf Extracts on Melanosis Prevention and Shelf-Life Extension of Refrigerated Pacific White Shrimp. Foods, 12(19), 3649.

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Nanoparticle Delivery of Doxorubicin Derivative

Cited 1 time

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PPD was synthesized from expired clinical samples of doxorubicin (FeRx Inc., Aurora, CO) as previously described.^{27 (link)} Fulvestrant was purchased from Selleckchem. Poly(d,l-lactide-co-2-methyl-2-carboxy-trimethylene carbonate)-graft-poly(ethylene glycol) (PLAC–PEG) was synthesized by ring-opening polymerization using a pyrenebutanol initiator to a molecular weight of 12 000 g/mol and conjugated with an average of three PEG chains/backbone (10 000 g/mol PEG) as previously described.²⁸ Polysorbate 80 (H2X, UP80) was purchased from NOF America Corporation. Vitamin E-PEG 1000 (VitEPEG), Pluronic F68, Pluronic F127, Brij L23, and Brij 58 were purchased from Sigma-Aldrich. McCoy’s 5A cell culture media, CholEsteryl BODIPY 542/563 C11, and Hoechst 33342 were purchased from Thermo Fisher Scientific. The SKOV-3 cell line was purchased from ATCC. Charcoal stripped fetal bovine serum and Hank’s balanced salt solution were purchased from Wisent Bioproducts.

Ganesh A.N., Logie J., McLaughlin C.K., Barthel B.L., Koch T.H., Shoichet B.K, & Shoichet M.S. (2017). Leveraging Colloidal Aggregation for Drug-Rich Nanoparticle Formulations. Molecular pharmaceutics, 14(6), 1852-1860.

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