Plasma HDL cholesterol, erythrocyte mean corpuscular volume (MCV), and the liver enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), and γ-glutamyltransferase (GGT) were determined as indirect biomarkers of alcohol consumption using standard laboratory methods. Plasma AST, ALT, and GGT were measured by a standardized enzymatic method (Cobas c501; Roche Diagnostics). Serum HDL cholesterol was measured by a homogeneous method (direct HDL-C, Aeroset System; Abbott Laboratories), and MCV concentrations were measured using a Coulter Counter STKS sum (Coulter Corporation).
Aeroset system
Manufactured by Abbott
Sourced in United States, Germany
The Aeroset System is a fully automated clinical chemistry analyzer designed for use in hospital and reference laboratories. It is capable of performing a wide range of routine and specialized clinical chemistry tests. The Aeroset System features advanced technology to ensure accurate and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Raybio human mouse rat ghrelin enzyme immunoassay, by RayBiotech (2 mentions)
Gentian cystatin c immunoassay, by Roche (2 mentions)
Cobas c501, by Roche (1 mentions)
Modular analytics, by Roche (1 mentions)
Architect c system, by Abbott (1 mentions)
5 bromo 2 deoxyuridine brdu, by Merck Group (1 mentions)
Lh 780 analyser, by Beckman Coulter (1 mentions)
Immunoradiometric assay, by Thermo Fisher Scientific (1 mentions)
Enzyme linked immunosorbent assay kit, by Thermo Fisher Scientific (1 mentions)
Modular p, by Roche (1 mentions)
Cystatin c immunoassay, by Roche (1 mentions)
Ektachem dry chemistry, by Kodak (1 mentions)
Mega clinical chemistry analyzer, by Merck Group (1 mentions)
Aldoctk 2, by DiaSorin (1 mentions)
15 protocols using aeroset system
1
Biomarkers of Alcohol Consumption
2
Comprehensive Metabolic and Inflammatory Profile
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Blood obtained after 12-h fast was immediately processed after collection and centrifuged at 3000× g and 4 °C for 5 min. Glucose, lipids, apolipoprotein A1 (ApoA1), apolipoprotein B, and gamma-glutamyl transpeptidase were analysed using standard assays (Aeroset System® Abbott Clinical Chemistry Abbott, Wiesbaden, Germany). Insulin was determined using an automated electrochemiluminescence immunoassay (Architect c8000®, Abbott Clinic-Chemistry, Abbott Park, IL, USA). Homeostatic model assessment (HOMA) was calculated as (fasting insulin (μU/mL) × fasting glucose (mg/dL)/405). High-sensitivity C-reactive protein and cystatin C were analysed by immunome-phelometry with a Behring 2 (Dade Behring, Marbung, Germany) nephelometer. Adipokines (leptin and adiponectin) and inflammatory markers such as interleukin 6 and tumor necrosis factor-α (TNF-α) were analysed using a Luminex 100 IS (Luminex Corporation, Austin, TX, USA).
3
Serum Biochemistry Panel Measurement
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Blood was collected for measurement of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin and cholinesterase by using the AEROSET System (Abbott Laboratories, Wiesbaden, Germany) according to the instructions of the manufacturers.
4
Lipid Profiling in RRSO Patients
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All individuals from both the RRSO and age-matched group provided a blood sample. Women from the age-matched group were asked to fast before providing a blood sample, while women from the RRSO group were not. For the RRSO group, total cholesterol, and HDL-C were measured with enzymatic methods (Roche/Hitachi modular analytics); for the age-matched control women, total cholesterol was estimated by a dry chemistry method (Eastman Kodak, Rochester, New York) and HDL-C was measured by a homogeneous method (direct HDL, Aeroset System; Abbott Laboratories, Abbott Park, Illinois). Non-HDL-C was calculated based on total cholesterol and HDL-C.
5
Comprehensive Biochemical Assessment
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The liver function parameters; Indirect Bilirubin (I-BILI), Direct Bilirubin (D-BILI), Total Bilirubin (T-BILI), Total Bile Acid (TBA), Gamma-glutamyl Transferase (GGT), Alkaline Phosphatase (ALP), Aspartate amino Transferase (AST), Alaline amino Transferase (ALT), lipid profile; Ligh Density Lipoprotein cholesterol (LDL-c), High Density Lipoprotein cholesterol (HDL-c), Triglyceride (TG), Total Cholesterol (TC), enzymes; Lactate Dehydrogenase (LDH), Creatine Kinase (CK), Amylase (AMS), Electrolytes; (PO43−, Ca2+, Cl−, Na+, K+, Mg2+, Fe2+) and other biochemical parameters such as Creatinine (Cr), Urea (UA), Blood Urea Nitrogen (BUN), Carbon Dioxide (CO2), Glucose (GLU), Globulin (GLB), Albumin (AlB) and Total Protein (TP) were investigated using a clinical auto-analyzer (Abbott Aeroset System). Before each assay, the auto-analyzer was calibrated.
6
Serum Biomarker Analysis in Rats
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Serum analyses were conducted at the Department of Clinical Chemistry, University of Goettingen according to the manufacturers' instructions (Abbott, Wiesbaden, Germany).
After collecting 5 ml of whole blood from the rats in the end of the experiment, it was centrifuged at 3,000 × g for 10 min. Serum was stored at −20°C. Creatine kinase (CK), Alanine transaminase (ALT), triglycerides, uric acid, Aspartate aminotransferase (AST/GOT), Cholesterol, glucose, and HDL were measured with the ARCHITECT c System and AEROSET System (Abbott, Wiesbaden, Germany).
After collecting 5 ml of whole blood from the rats in the end of the experiment, it was centrifuged at 3,000 × g for 10 min. Serum was stored at −20°C. Creatine kinase (CK), Alanine transaminase (ALT), triglycerides, uric acid, Aspartate aminotransferase (AST/GOT), Cholesterol, glucose, and HDL were measured with the ARCHITECT c System and AEROSET System (Abbott, Wiesbaden, Germany).
7
Plasma Calcium Quantification Protocol
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Blood samples were collected in heparinised tubes immediately prior to euthanization. The samples were centrifuged at 3000 rpm (1400g) at 4 °C for 10 min to separate the plasma. Plasma samples were stored at − 70 °C until analysis of total calcium (mmol/L) was performed (Calcium Architect cSystems, Aeroset System, Abbott Laboratories, Solna, Sweden).
8
Hepatocyte Proliferation Assessment in Rats
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Rats were sacrificed postoperatively day 1, 2, 3 and 7. For detecting hepatocyte proliferation in the FLR, rats were injected intravenously with a single dose of 50 mg/kg 5-bromo-2-deoxyuridine (BrdU, SIGMA-ALDRICH, St. Louis, USA) one hour before sacrifice.
Blood collection was performed under anesthesia and liver tissue was harvested. The wet weight of remnant liver was measured and the liver weight/body weight ratio was calculated using the following formula: individual liver lobe weight (g)/body weight (g) × 100%.
Serum was isolated from the blood. The serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured by using the AEROSET System (Abbott Laboratories, Wiesbaden, Germany).
Blood collection was performed under anesthesia and liver tissue was harvested. The wet weight of remnant liver was measured and the liver weight/body weight ratio was calculated using the following formula: individual liver lobe weight (g)/body weight (g) × 100%.
Serum was isolated from the blood. The serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were measured by using the AEROSET System (Abbott Laboratories, Wiesbaden, Germany).
9
Coronary Angiography Blood Biomarkers
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Fasting blood samples were collected one day before coronary angiography for the evaluation of full blood count, glucose, creatinine level, LDL cholesterol, high-density lipoprotein (HDL) cholesterol, triglyceride, and serum bilirubin level. Complete blood counts were performed using a Beckman Coulter LH 780 analyser (Miami, FL, USA). Serum bilirubin levels were determined by the colorimetric diazo method on the Aeroset System (Abbott Laboratories, Abbott Park, IL, USA).
10
Fasting Biochemical Parameters and Hormones
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After a 12 h fast, baseline laboratory parameters, including serum glucose, creatinine, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol, triglycerides (TGs), high-density lipoprotein (HDL) cholesterol, and low-density lipoprotein (LDL) cholesterol, were measured using the auto analyzer system (Aeroset System Abbott, Abbott Laboratories, Diagnostic Division, Chicago, IL, USA) [37 (link)].
Further, once plasma samples were collected following previous procedures described elsewhere [21 (link)], leptin levels were measured by means of an enzyme immunoassay (ELISA) kit (cat. No. EH0216; FineTest, Wuhan, China), insulin was analyzed by an immunoradiometric assay (BioSource International, Camarillo, CA, USA), and ghrelin was determined by an enzyme-linked immunosorbent assay kit (cat. No. EH0355; FineTest, Wuhan, China). The methods of analysis and concentration measurements followed established procedures [21 (link)].
Further, once plasma samples were collected following previous procedures described elsewhere [21 (link)], leptin levels were measured by means of an enzyme immunoassay (ELISA) kit (cat. No. EH0216; FineTest, Wuhan, China), insulin was analyzed by an immunoradiometric assay (BioSource International, Camarillo, CA, USA), and ghrelin was determined by an enzyme-linked immunosorbent assay kit (cat. No. EH0355; FineTest, Wuhan, China). The methods of analysis and concentration measurements followed established procedures [21 (link)].
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