Enterococcus hirae

Manufactured by American Type Culture Collection

Sourced in United States

Enterococcus hirae is a bacterial strain available from the American Type Culture Collection (ATCC). It is a Gram-positive, catalase-negative, and facultatively anaerobic coccus. Enterococcus hirae is commonly used in microbiology research and applications.

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7 protocols using enterococcus hirae

Antimicrobial Activity of Freeze-Dried Fruit

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The antimicrobial activity of the freeze-dried fruit tissue was tested on the following strains of microorganisms: (1) Gram positive bacteria Staphylococcus aureus (ATCC 9538), Bacillus subtilis (ATCC 6633), Enterococcus hirae (ATCC 10542), and Enterococcus faecalis (ATCC 29212); (2) Gram negative bacteria Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 15442), Vibrio harveyi (ATCC 12126), and Proteus mirabilis (ATCC 2011); and (3) yeast Candida albicans (ATCC 10231). All microorganisms were obtained from the culture collection of the Department of Biotechnology and Food Microbiology (Wrocław). The bacteria were grown in nutrient broth medium at 37 °C, except B. subtilis (ATCC 6633) which was grown at 30 °C. The yeast was grown in Yeast Extract Peptone Glucose (YPD) medium at 30 °C. The agar was added to the medium at a concentration of 2% when it was needed.

Lachowicz S., Wiśniewski R., Ochmian I., Drzymała K, & Pluta S. (2019). Anti-Microbiological, Anti-Hyperglycemic and Anti-Obesity Potency of Natural Antioxidants in Fruit Fractions of Saskatoon Berry. Antioxidants, 8(9), 397.

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Antimicrobial Activity Evaluation Protocol

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β-Hemolytic Streptococcus group A PCM 465, Streptococcus group G and Corynebacterium diphtheriae came from Department of Pharmaceutical Microbiology of Medical University of Gdańsk collection, Staphylococcus aureus ATCC6538, Staphylococcus aureus MRSA ATCC43300, Staphylococcus epidermidis ATCC14990, Enterococcus hirae ATCC10541, Escherichia coli ATCC8739 and Candida albicans ATCC10231 were obtained from the ATCC collection (ATCC, Manassas, VA, USA).

Stefanowicz-Hajduk J., Hering A., Gucwa M., Hałasa R., Soluch A., Kowalczyk M., Stochmal A, & Ochocka R. (2020). Biological activities of leaf extracts from selected Kalanchoe species and their relationship with bufadienolides content. Pharmaceutical Biology, 58(1), 732-740.

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Antimicrobial Efficacy of ADC Gel

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A total of 10 specimens were used. The antimicrobial activity of ADC gel was
evaluated in vitro against gram-positive and gram-negative bacteria and
Candida albicans. ADC gel (50 μL) was added in the
centre of a Petri dish containing TSA (Tryptone Soya Agar), previously
contaminated at the surface with 100 μL of a microbial pool containing
Staphylococcus aureus ATCC 6538, Escherichia
coli ATCC 10536, Pseudomonas aeruginosa ATCC
15442, Enterococcus hirae ATCC 10541 and Candida
albicans ATCC 1023 in concentration of the order of 1.5 − 5 ×
10¹⁰ cfu/mL (Diagnostic International Distribution S.p.A,
Milan, Italy). The minimum inhibitory concentration of a microbial pool
(including periodontal pathogen bacteria) in concentration of 10,000,000
cfu/mL was 0.3% w/w. The results of the in vitro test show the remarkable
inhibition of the microbial growth observed after 24 h of incubation at
36°C.

Lauritano D., Palmieri A., D’Orto O., Bolzoni A., Ottria L., Gargari M., Di Girolamo M, & Candotto V. (2019). The use of a new chemical device based on silver and cationic surfactants as a new approach for daily oral hygiene: A preliminary study on a group of periodontal patients. International Journal of Immunopathology and Pharmacology, 33, 2058738419868101.

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Microbial Strains for Antimicrobial Assays

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Microorganisms used in this study were as follows: Gram-positive bacteria: Staphylococcus aureus NCTC 4163, S. aureus ATCC 25923, S. aureus ATCC 6538, S. aureus ATCC 29213, S. epidermidis ATCC 12228, S. epidermidis ATCC 35984, Bacillus subtilis ATCC 6633, Enterococcus hirae ATCC 9341, Enterococcus faecalis ATCC 29212; Gram-negative rods: E. coli ATCC 8196, Pseudomonas aeruginosa ATCC 15442, Bordetella bronchiseptica ATCC 4617 and yeasts: Candida albicans ATCC 10231, C. albicans ATCC 30028, C. albicans clinical isolate 26, C. albicans clinical isolate 18.
All microorganisms used were obtained from the collection of the Department of Pharmaceutical Microbiology, Medical University of Warsaw, Poland.

Jóźwiak M., Stępień K., Wrzosek M., Olejarz W., Kubiak-Tomaszewska G., Filipowska A., Filipowski W, & Struga M. (2018). Synthesis, Structural Studies and Biological Evaluation of Connections of Thiosemicarbazide, 1,2,4-Triazole and 1,3,4-Thiadiazole with Palmitic Acid. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 822.

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Microbial Strain Characterization Protocol

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Nine test strains and one internal standard strain were used. The following test strains were obtained from the American Type Culture Collection (ATCC); Kocuria rhizophila (ATCC 9341), Escherichia coli (ATCC 33588), Enterococcus hirae (ATCC 8043), Bacillus subtilis (ATCC 6051), Enterococcus faecalis (ATCC 47077), Streptococcus mutans (ATCC 25175), Listeria innocua (ATCC33090), and Staphylococcus epidermidis (ATCC12228). Ralstonia solanacearum (T-11 strain) [20 ] was isolated from Tomato plants. Lactobacillus sakei (NBRC 15893) used for internal standard was obtained from the National Institute of Technology and Evaluation (NBRC). All these strains were cultured in a BD Difco Nutrient broth supplemented with 5 g/L yeast extract (NYB).

Kawai Y., Ozawa N., Fukuda T., Suzuki N, & Mikata K. (2022). Development of an efficient antimicrobial susceptibility testing method with species identification by Nanopore sequencing of 16S rRNA amplicons. PLoS ONE, 17(2), e0262912.

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Antimicrobial Susceptibility Screening

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The microorganisms used in this study represent pathogenic species commonly associated with healthcare and household environments. Microorganisms were obtained from MediMark Europe and TCS Biosciences Ltd. and consisted of the Gram-positive strains Enterococcus hirae (ATCC 10541) (MediMark), Bacillus cereus (ATCC 10876) (TCS), and Staphylococcus aureus (ATCC 6538) (MediMark); the Gram-negative strains Escherichia coli (ATCC 10536) (MediMark), Pseudomonas aeruginosa (ATCC 15442) (TCS), and Legionella pneumophila (ATCC 33152) (TCS); and the fungi Aspergillus brasiliensis (ATCC 16404) (MediMark), and Candida albicans (ATCC 10231) (MediMark). Bacteria and fungi were maintained in the Department of Chemical Biology and Bioimaging, Wroclaw University of Science and Technology. All bacterial strains were subcultured from the original culture and maintained on Nutrient LAB-AGAR™ (Biocorp Poland) plates at 4 °C and grown at 37 °C when required. All fungal strains were subcultured from the original culture and kept in Sabouraud Dextrose LAB-AGAR™ (Biocorp Poland) plates at 4 °C and grown at 28 °C when required. The Alamarblue® reagent was obtained from Bio-Rad-Antibodies UK. Microplates were obtained from NEST Biotechnology Co., Ltd. China.

Surowiak A.K., Sowała M., Talma M., Groborz K., Balcerzak L., Lochyński S, & Strub D.J. (2022). Cytotoxicity, early safety screening, and antimicrobial potential of minor oxime constituents of essential oils and aromatic extracts. Scientific Reports, 12, 5319.

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Antimicrobial Evaluation of Bacterial Strains

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Staphylococcus aureus ATCC 6538,Listeria monocytogenes ATCC 7644, Escherichia coli ATCC 10536; Salmonella typhimurium ATCC 13311,Enterococcus hirae ATCC 10541, Proteus mirabilis ATCC 13315, Bacillus subtilis ATCC 6633, Shigella dysenteriae CECT 457, Pseudomonas aeruginosa ATCC 27853, Bifidobacterium animalis sbsp lactis (Bb12), and Lactobacillus rhamnosus LbRE-LSAS were obtained from the LMBAF laboratory (University of Mostaganem, Mostaganem, Algeria).

Mokhtar M., Bouamar S., Di Lorenzo A., Temporini C., Daglia M, & Riazi A. (2021). The Influence of Ripeness on the Phenolic Content, Antioxidant and Antimicrobial Activities of Pumpkins (Cucurbita moschata Duchesne). Molecules, 26(12), 3623.

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