Prism statistical analysis software

Manufactured by GraphPad

Sourced in United States

Prism is a scientific graphing and statistical analysis software. It provides tools for data organization, visualization, and statistical analysis. Prism allows users to create high-quality graphs and perform a variety of statistical tests on their data.

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Lab products found in correlation

Maxisorp plate, by Thermo Fisher Scientific (1 mentions) Spss v 28 for windows, by IBM (1 mentions) Click it plus edu alexa fluor 647 imaging kit, by Thermo Fisher Scientific (1 mentions) Tcs sp8 confocal microscope, by Leica (1 mentions) Celltiter glo, by Promega (1 mentions) Infinite 200 plate reader, by Tecan (1 mentions) Stata se 14, by StataCorp (1 mentions)

Close spelling variants of this product

Prism 9 statistical analysis software PRISM statistical analysis Prism statistical software Prism analysis software Statistical analysis software Prism statistical Prism analysis Prism software

27 protocols using prism statistical analysis software

ELISA Antibody Titer Quantification

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Antigens (0.5 μg/ml) were adsorbed at 4°C overnight on Maxisorp plates (NUNC, Rochester, NY), and unoccupied sites were blocked with 1% BSA for a minimum of 1 hr. Serial 2‐fold diluted serum samples were added to individual wells (100 μl/well) and incubated for 2 hr at room temperature. Following washing, isotype‐specific antibody conjugated to horseradish peroxidase (1:6000 dilution; Southern Biotech, Birmingham, AL) was added, and following washing, the wells were developed with TMB substrate (Pierce, Rockford, IL), and the reaction stopped with 50 μl H₂SO₄ (1.0 M). Absorbances were measured at 450 nm minus the absorbance at 570 nm to correct for plate abnormalities. The resulting data for each sample were plotted to obtain a curve of the reciprocal dilution vs. the A₄₅₀–A₅₇₀ measurement. The antibody titre was determined as the midpoint of the dilution curve as defined by EC₅₀ calculations using Prism statistical analysis software (GraphPad Software, La Jolla, CA). The mean of the EC₅₀ for each cohort was used as the antibody titre.

Huang N., Shimomura E., Yin G., Tran C., Sato A., Steiner A., Heibeck T., Tam M., Fairman J, & Gibson FC I.I.I. (2019). Immunization with cell‐free‐generated vaccine protects from Porphyromonas gingivalis‐induced alveolar bone loss. Journal of Clinical Periodontology, 46(2), 197-205.

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Statistical Analysis of Experimental Data

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All experiments were performed 2–3 times and data from individual experiments were combined, imported in Prism statistical analysis software (Graphpad Software, La Jolla, CA), and the mean ± standard error of the mean for each group was determined. One-way ANOVA and Tukey post-tests, or Two-way ANOVA with Bonferroni post-test were performed as indicated. A P < 0.05 was considered significant.

Papadopoulos G., Shaik-Dasthagirisaheb Y.B., Huang N., Viglianti G.A., Henderson A.J., Kantarci A, & Gibson FC I.I.I. (2016). Immunologic Environment Influences Macrophage Response to Porphyromonas gingivalis. Molecular oral microbiology, 32(3), 250-261.

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Statistical Analysis of Experimental Data

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Statistical analysis was performed to assess the difference using an unpaired Student's t-test, two-way ANOVA followed by multiple comparisons by Prism statistical analysis software (GraphPad Software, San Diego, CA). Data are presented as the mean ± SD. Significance is indicated as follows: *P < 0.05 or n.s. for not significant.

Chen X., Wang C., Sun N., Pan S., Li R., Li X., Zhao J., Tong H., Tang Y., Han J., Qiao J., Qiu H., Wang H., Yang J, & Ikezoe T. (2021). Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production. Theranostics, 11(10), 4655-4671.

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BMP Signaling Regulation of Tumor Growth

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All in vitro and ex vivo experiments were performed at least in triplicates. A minimum of 6 in vivo xenograft models were used for each group for tumour volume and invasion analysis, and three xenograft tumours from each group were used for pSMAD1,5,8 expression analysis. Mean values are presented with error bars corresponding to ± SD. Statistical analysis was performed by using Prism statistical analysis software (GraphPad). Significance is indicated as ∗∗∗p < 0.001; ∗∗p < 0.01; ∗p < 0.05.

Merve A., Dubuc A.M., Zhang X., Remke M., Baxter P.A., Li X.N., Taylor M.D, & Marino S. (2014). Polycomb group gene BMI1 controls invasion of medulloblastoma cells and inhibits BMP-regulated cell adhesion. Acta Neuropathologica Communications, 2, 10.

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Quantifying Cellular Responses to Stimuli

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Unless specified in the text, all the graphs represent population mean ± SD. Statistical analysis was performed with Prism statistical analysis software (GraphPad), Student's t test for single comparisons and ANOVA for multiple comparisons were applied. Significance is indicated in figures as ^∗∗∗p < 0.001, ^∗∗p < 0.01, and ^∗p < 0.05.

Dibenedetto S., Niklison-Chirou M., Cabrera C.P., Ellis M., Robson L.G., Knopp P., Tedesco F.S., Ragazzi M., Di Foggia V., Barnes M.R., Radunovic A, & Marino S. (2017). Enhanced Energetic State and Protection from Oxidative Stress in Human Myoblasts Overexpressing BMI1. Stem Cell Reports, 9(2), 528-542.

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Neonatal Hyperoxia Survival Analysis

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Significance was determined by two-tailed Student’s t-test using GraphPad PRISM statistical analysis software. Chi-square analysis (software R, package “survival”) was used for the comparison of survival data of all hyperoxia-exposed litters. All data are presented as mean ± SEM. Values of p<0.05 were considered significant.

Chao C.M., Yahya F., Moiseenko A., Tiozzo C., Shrestha A., Ahmadvand N., Agha E.E., Quantius J., Dilai S., Kheirollahi V., Jones M., Wilhem J., Carraro G., Ehrhardt H., Zimmer K.P., Barreto G., Ahlbrecht K., Morty R.E., Herold S., Abellar R.G., Seeger W., Schermuly R., Zhang J.S., Minoo P, & Bellusci S. (2016). Fgf10 deficiency is causative for lethality in a mouse model of bronchopulmonary dysplasia. The Journal of pathology, 241(1), 91-103.

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In vitro biological replicates analysis

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For in vitro
studies, n represents the number of biological replicates.
Technical replicates refer to experiments carried out with different
passage cells but identical experimental conditions. Data were presented
as mean ± standard deviation (s.d.) and analyzed by Prism statistical
analysis software (GraphPad v. 7.03).

Blokpoel Ferreras L.A., Chan S.Y., Vazquez Reina S, & Dixon J.E. (2020). Rapidly Transducing and Spatially Localized Magnetofection Using Peptide-Mediated Non-Viral Gene Delivery Based on Iron Oxide Nanoparticles. ACS Applied Nano Materials, 4(1), 167-181.

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Analyzing Behavioral Data with ANOVA

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Behavior data were analyzed using a within-subjects repeated-measures ANOVA followed by a Tukey test for post hoc analyses using Graphpad Prism Statistical Analysis software. Analyses of acquisition data included all data points, whereas analysis of all subsequent cued-retention trials limited the data to the first trial only. Statistical significance was defined at p < 0.05.

Anderson D.W., Mettil W, & Schneider J.S. (2016). Effects of Low Level Lead Exposure on Associative Learning and Memory in the Rat: Influences of Sex and Developmental Timing Of Exposure. Toxicology letters, 246, 57-64.

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Statistical Analysis of Experimental Data

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Data were analyzed by GraphPad prism statistical analysis software using Student’s t-test and one-way analysis of variance (ANOVA).

Ye L., Gao Z, & Rohani S. (2023). Intervertebral disk regeneration in a rat model by allopurinol-loaded chitosan/alginate hydrogel. Biomolecules and Biomedicine, 23(4), 661-670.

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Nonlinear Regression Analysis of Drug Potency

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The CD concentrations were converted to Log10 values, and curve fit and IC50 values were calculated using the Nonlinear regression One site—Fit logIC50 function of the Prism software, version 7 (GraphPad Software, San Diego, CA, USA). Statistical significance was assessed by one-way analysis of variance (ANOVA) and post-hoc Dunnett’s test for multiple comparisons against the control (vehicle-exposed) group. A value of p < 0.05 was considered statistically significant. All data are presented as mean ± standard error of mean (SEM). Statistical analysis was performed using Prism statistical analysis software, version 7 (GraphPad Software, CA, USA) or SPSS for Windows v.28 (SPSS Inc., Chicago, IL, USA).

Alboni S., Secco V., Papotti B., Vilella A., Adorni M.P., Zimetti F., Schaeffer L., Tascedda F., Zoli M., Leblanc P, & Villa E. (2023). Hydroxypropyl-β-Cyclodextrin Depletes Membrane Cholesterol and Inhibits SARS-CoV-2 Entry into HEK293T-ACEhi Cells. Pathogens, 12(5), 647.

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