Metuzumab was supplied by Pacific Meinuoke Biopharmaceutical Co., Ltd. RPMI medium 1640 (72,400-047) was purchased from Gibco (Grand Island, NY, USA). Hypoxanthine (H9377-5G) and chloroquine (C6628-25G) were purchased from Sigma–Aldrich (St. Louis, MO, USA). Clodronate liposomes were purchased from Liposoma B.V. (Amsterdam, The Netherlands). IgG from human serum was purchased from Genia (Beijing, China). Anti-human CD235a PE antibody (12-9987-82, RRID: AB_466,300) was purchased from Thermo-Fisher (Waltham, MA, USA). CD147-his protein and anti-meplazumab polyclone antibody were supplied by Fourth Military Medical University (Xi'an, China). Human IgG-heavy and light chain monkey-adsorbed antibody conjugate horseradish peroxidase (HRP) was purchased from Bethyl (Montgomery, AL, USA). 125I-Meplazumab was supplied by MITRO Biotech Co., Ltd. (Nanjing, China). The CD31 antibody (11265-1-AP) was purchased from Proteintech Group, Inc. (Wuhan, China).
Chloroquine c6628 25g
Manufactured by Merck Group
Sourced in United States
Chloroquine (C6628-25G) is a laboratory compound provided by Merck Group. It is a crystalline solid that is soluble in water and organic solvents. Chloroquine is commonly used in research applications, but its core function is not to be extrapolated or interpreted in this response.
Automatically generated - may contain errors
Lab products found in correlation
Clodronate liposome, by Liposoma (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
Anti lc3b antibody, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Chloroquine, by Merck Group (1 mentions)
Crizotinib, by Merck Group (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Ab32072, by Abcam (1 mentions)
2 protocols using chloroquine c6628 25g
1
Metuzumab-based Cellular Interaction Assay
2
Autophagy Regulation by Crizotinib and Chloroquine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies used in this study included anti-MYC (Y69, 1:2000, ab32072) which was obtained from Abcam (Cambridge, MA, USA). Anti-β-actin (1:2000, sc-47778) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-LC3B antibody was from Sigma-Aldrich (1:2000, Lot# 048M4810V). Beclin-1 (1:4000, #3738) and ATG7 (1:1000, #8558) antibodies were purchased from Cell Signaling (Danvers, MA, USA). Secondary antibodies used were HRP-conjugated anti-mouse (1:2000, #7076) and anti-rabbit (1:2000, #7074) (Cell Signaling). crizotinib (PZ0191-5MG) and chloroquine (C6628-25G) were purchased from Sigma-Aldrich. All treatments were performed according to the manufacturer instructions. Stock solutions of crizotinib (1 mM) were prepared in dimethyl sulfoxide (DMSO, Sigma-Aldrich) and stock solution of chloroquine (50 mM) was prepared in water. They were then diluted into the desired concentration for further drug treatment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!