Raw 264.7 bcrc 60001

Six components of S. flexibilis including, 11-dehydrosinulariolide (SC-2), sinulariolide (SC-4), dendronpholide F (SC-6), 3,4:8,11-bisepoxy-7-acetoxycembra-15(17)-en-1,12-olide (SC-7), sinularin (SC-9), and dihydrosinularin (SC-10), were isolated and identified by Prof. Jui-Hsin Su (National Museum of Marine Biology and Aquarium, Pintung, Taiwan) [23 (link)]. Dimethyl sulfoxide (DMSO), LPS, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), napthyl-ethylene-dihydrochloride, sulfanilamide, and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). RAW 264.7 (BCRC 60001) and HaCaT cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan). Dulbecco’s Modified Eagle Medium (DMEM), fetal bovine serum (FBS), antibiotics, and glutamine were purchased from GIBCO BRL (Grand Island, NY, USA). The bacterial culture equipment, including an anaerobic atmosphere by MGC AnaeroPack-Jar and MGC AnaeroPack-Anaero, were purchased from Mitsubishi Gas Chemical (Tokyo, Japan). Blood agar plates (BAPs), BactoTM tryptic soy agar (TSA), and BactoTM tryptic soy broth (TSB) were purchased from Difco (Detroit, MI, USA).

Mesenchymal stem cells (MSCs) were isolated from bone marrow of C57BL/6 female mice according to the guidelines of the Association for Assessment and Accreditation of Laboratory Animal Care International. C57BL/6 MSCs were also purchased from Thermo Fisher Scientific Life Sciences (Waltham, MA, http://www.thermofisher.com). MSCs stably expressing the LacZ gene (β‐Gal‐MSCs) via a lentiviral system, as previously described [11]. Cell lines were maintained in Dulbecco's modified Eagle's medium/Ham's nutrient mixture F‐12 (DMEM/F12) (Thermo Fisher), supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher), 2 mM l‐glutamine, and 1% penicillin/streptomycin. Human 14‐week male embryonal lung cell line MRC‐5 (BCRC‐60023) was purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan, http://www.bcrc.firdi.org.tw). MRC‐5 cells were maintained in Eagle's Minimal Essential Medium (MEM) (Thermo Fisher), supplemented with 10% FBS and 1% penicillin/streptomycin and were incubated at 37°C in a 5% CO₂ incubator. Mouse macrophage‐like cell line RAW264.7 (BCRC‐60001) was purchased from Bioresource Collection and Research Center. Cell lines were maintained in DMEM containing 10% heat‐inactivated FBS.