Rabbit anti vcam 1

Manufactured by Cell Signaling Technology

Sourced in United States

Rabbit anti-VCAM-1 is a primary antibody that specifically recognizes the vascular cell adhesion molecule-1 (VCAM-1) protein. VCAM-1 is a cell surface receptor that mediates the adhesion of leukocytes to the vascular endothelium. This antibody can be used to detect and quantify VCAM-1 expression in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Lyophilized bovine thrombin, by Merck Group (1 mentions) Picrosirius red staining, by Abcam (1 mentions) Rabbit anti aquaporin 5, by Abcam (1 mentions) Mouse anti icam 1, by Abcam (1 mentions) Mouse anti beta 3 tubulin, by Abcam (1 mentions) ε aminocaproic acid εaca, by Merck Group (1 mentions) Sulfosuccinimidyl 6 3 2 pyridyldithio propionamido hexanoate sulfo lc spdp, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated anti rabbit igg secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 conjugated anti mouse igg secondary antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti cytokeratin 7, by Abcam (1 mentions) Rabbit anti n cadherin, by Abcam (1 mentions) Millex syringe filter, by Merck Group (1 mentions) Rabbit anti ki67, by Abcam (1 mentions) To pro 3 iodide, by Thermo Fisher Scientific (1 mentions) Calcium chloride, by Merck Group (1 mentions) Mouse anti claudin 5, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Sterile culture ware, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 and 555, by Thermo Fisher Scientific (1 mentions) Donkey anti rabbit, by GE Healthcare (1 mentions)

6 protocols using rabbit anti vcam 1

Fibrin Hydrogel Scaffold Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lyophilized human fibrinogen and Millex syringe filter were purchased from EMD Millipore (Billerica, MA). Lyophilized bovine thrombin, calcium chloride and ε-aminocaproic acid (εACA) were purchased from Sigma-Aldrich (St. Louis, MO). Sulfosuccinimidyl 6-(3’-(2-pyridyldithio)propionamido)hexanoate (Sulfo-LC-SPDP) was purchased from Thermo Fisher Scientific (Newington, NH). Human Factor XIII was purchased from Enzyme Research Laboratories (South Bend, IN). Dialysis membrane was purchased from Spectrum Laboratories (Rancho Dominguez, CA). Picrosirius red staining was purchased from Abcam (Cambridge, MA). TO-PRO-3 iodide, Alexa Fluor 488 conjugated anti-rabbit IgG secondary antibody and Alexa Fluor 568 conjugated anti-mouse IgG secondary antibody were purchased from Invitrogen (Carlsband, CA). Rabbit anti-aquaporin 5, mouse anti-cytokeratin 7, rabbit anti-Ki-67, mouse anti-ICAM-1, rabbit anti-N-cadherin and mouse anti-beta III tubulin were purchased from Abcam. Rabbit anti-VCAM-1 was purchased from Cell Signaling Technology (Danvers, MA). Peptides were synthesized by University of Utah DNA/Peptide synthesis core facility. Female C57BL/6 mice at 6 weeks old weighing 15–19 g were purchased from the Jackson Laboratory (Bar Harbor, ME).

Nam K., Dean S.M., Brown C.T., Smith RJ J.r., Lei P., Andreadis S.T, & Baker O.J. (2019). Synergistic Effects of Laminin-1 Peptides, VEGF and FGF9 on Salivary Gland Regeneration. Acta biomaterialia, 91, 186-194.

+ Open protocol

+ Expand

Antibody Sources for Cell-Cell Adhesion Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used in this study were obtained from the following sources: Rabbit anti-ZO-1 (#8193), rabbit anti-claudin-3 (#341700), rabbit anti-VE-cadherin (#D87F2), rabbit anti-VCAM-1 (#12367), mouse PECAM-1 (#89C2) from Cell Signaling Technology (Danvers, MA, USA); mouse anti-E-selectin (#S 9555), β-actin (#A5441) from Sigma-Aldrich (St. Louis, MO, USA); donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) HRP-linked secondary antibodies from GE Healthcare (Piscataway, NJ, USA); mouse anti-claudin 5 (#35-2500), goat anti-rabbit (#A11008) and anti-mouse (#A21422) conjugated to Alexa Fluor® 488 and 555 from Invitrogen (Camarillo, CA, USA). Sterile cultureware was obtained from Fisher Scientific (Pittsburgh, PA, USA), while other reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-Rad laboratories (Hercules, CA, USA).

Naik P., Fofaria N., Prasad S., Sajja R.K., Weksler B., Couraud P.O., Romero I.A, & Cucullo L. (2014). Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?. BMC Neuroscience, 15, 51.

+ Open protocol

+ Expand

Western Blot Analysis of Protein Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were collected in 1× RIPA buffer containing 1 mM PMSF and sonicated for 10 s. Protein concentrations were measured using the bicinchoninic acid (BCA) assay (Pierce). Equal amounts of protein (20 μg/lane) were loaded onto 4%–15% precast protein gels (Bio-Rad) and transferred to polyvinylidene difluoride (PVDF, Bio-Rad) membranes. Membranes were blocked for 1 hr with TBS/0.1%-Tween buffer plus 5% (w/v) non-fat dried milk and incubated overnight at 4°C with primary antibodies dissolved in blocking buffer. Membranes were incu-bated with secondary antibodies for 1 hr and developed using a Pierce^® ECL Western blotting detection kit (Thermo Scientific) and a ChemiDoc XRS System (Bio-Rad). Image lab software was used to quantitate Western blot signals. The following primary antibodies were used in this study: mouse anti-CaMKK β (1:200; Santa Cruz Biotechnology), rabbit anti-SIRT1 (1:1000; Cell Signaling), rabbit anti-phospho-SIRT1 (1:1000; Cell Signaling), rabbit anti-phospho-CAMKIV (1:1000; Abcam), rabbit anti-eNOS (1:1000; Cell Signaling), rabbit anti-VCAM-1 (1:1000; Cell Signaling), rabbit anti-CD54/ICAM1 (1:1000;Cell Signaling) and mouse anti-β-actin (1:5000; Sigma-Aldrich). HRP (horseradish peroxidase)-conjugated anti-rabbit IgG (1:2000; Vector Laboratories) and HRP-conjugated anti-mouse IgG (1:2000; Vector Laboratories) were used as secondary antibodies.

Sun P., Bu F., Min J.W., Munshi Y., Howe M.D., Liu L., Koellhoffer E.C., Qi L., McCullough L.D, & Li J. (2018). Inhibition of calcium/calmodulin-dependent protein kinase kinase (CaMKK) exacerbates impairment of endothelial cell and blood–brain barrier after stroke. The European journal of neuroscience, 49(1), 27-39.

+ Open protocol

+ Expand

Western Blotting Analysis of Inflammatory Markers

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blotting was done, following the previously mentioned method [18 ,19 (link)]. Hippocampal tissues were extracted by homogenization using lysis buffer (pH, 7.6) After separating 30 μg protein using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the protein was moved to a nitrocellulose membrane (GE Healthcare Bio-Sciences, Pittsburgh, PA, USA). The membrane was treated with 5% skimmed milk powder (BD Difco, Detroit, MI, USA) for blocking, and incubated with 0.1% Tween-20 (Sigma Aldrich Co.) for 1 hour at room temperature. The membrane was treated with the primary antibodies such as rabbit anti-VCAM-1 (1:1,000, Cell Signaling Technology), rabbit anti-ICAM-1 (1:1,000, Cell Signaling Technology), mouse anti-TNF-α (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-IL-6 (1:1,000; Santa Cruz Biotechnology), and mouse anti-IL-1β (1:1,000; Santa Cruz Biotechnology). Membranes were subsequently incubated for l hour with peroxidase-tagged secondary antibodies. Enhanced chemiluminescence detection kit (DoGen, Seoul, Korea) was used for visualization of protein, and quantitative analysis of bands was calculated using Image-ProPlus program (Media Cybernetics, Rockville, MD, USA).

Park S.S., Kim T.W., Sung Y.H., Park Y.J., Kim M.K, & Shin M.S. (2021). Treadmill Exercise Ameliorates Short-term Memory Impairment by Suppressing Hippocampal Neuroinflammation in Poloxamer-407-Induced Hyperlipidemia Rats. International Neurourology Journal, 25(Suppl 2), S81-S89.

+ Open protocol

+ Expand

Immunofluorescence Staining of Frozen Liver Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen liver sections (10µm) were incubated with rabbit anti-VCAM-1 (1:200) or rabbit anti-cleaved caspase-3 Asp175 (5A1E) (1:100, Cell Signalling Technology USA) and sheep anti-von Willebrand Factor (vWF; 1:100) (Abcam, MA, USA) overnight at 4°C. Alexa Fluor^® 647 Donkey anti-Rabbit IgG (H+L) and Alexa Fluor 488 Donkey anti-Sheep IgG (H+L) (1:900; Thermo Fisher Australia) were used as secondary antibodies. Images were captured using the Nikon TiE microscope (Monash Micro Imaging Platform).

Pereira M., Lee N.T., Noonan J., Willcox A.E., Calvello I., Georgy S.R., Selan C., Chia J.S., Hauw W., Wang X., Peter K., Robson S.C., Nandurkar H.H, & Sashindranath M. (2021). Early Endothelial Activation in a Mouse Model of Graft vs Host Disease Following Chemotherapy. Frontiers in Immunology, 12, 708554.

+ Open protocol

+ Expand

Curcumin Modulates TNF-α-Induced Inflammatory Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

HUVECs pre-exposed to curcumin or vehicle were harvested at the end of a 4-hour-TNF- activation period and washed twice with ice-cold PBS. Total proteins were extracted using lysis buffer containing 50 mmol/L Tris pH 7.8, 150 mmol/L NaCl, 0.5% sodium deoxycholate, 1% NP40, antiprotease and anti-phosphatase. Protein concentration was determined using BCA protein assay reagent Kit (Interchem). Lysates were loaded onto a 10% SDS-polyacrylamide gel for electrophoresis and then transferred onto immobilon-P membrane (GE Healthcare). The membrane was incubated in 5% (wt/vol) dried milk protein in TBS containing 0.05% Tween-20 for 1 hour, and then further reacted with primary antibodies: rabbit anti-iCAM (Santa Cruz, 1:1000), rabbit anti-vCAM-1 (1:1000, GTX), rabbit anti-NFB total (1:1000, Cell Signaling), rabbit anti-NFB phosphor-ser536 (1:1000, Cell Signaling), rabbit anti-IB total (1:1000, Cell Signaling) and rabbit anti-IB phosphor-ser32
(1:1000, Cell Signaling). After extensive washes, membrane was incubated with anti-rabbit IgG antibody conjugated to HRP (1:5000, Santa Cruz, USA). Protein bands were visualized using ECL detection kit (Millipore, USA) and then analyzed using Image J software (www.imagej.nih.gov).
Graphs represent protein level expressed as the mean +/-standard deviation of 4 independent experiments.

Monfoulet L.E., Mercier S., Bayle D., Tamaian R., Barber-Chamoux N., Morand C, & Milenkovic D. (2017). Curcumin modulates endothelial permeability and monocyte transendothelial migration by affecting endothelial cell dynamics. Free radical biology & medicine, 112.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!