Cell observer z1 system

Manufactured by Zeiss

The Cell Observer.Z1 system is a microscope designed for live-cell imaging. It provides high-resolution, time-lapse imaging capabilities for the observation and analysis of cellular processes.

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Lab products found in correlation

Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Axio Observer Z1 Live-Cell imaging system Microscope Cell Observer Z1 system Cell Observer Z1 Observer Z1 Cell Observer System Cell Observer

2 protocols using cell observer z1 system

Immunostaining for SLFN5 and V5 proteins

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For immunostaining, cells were fixed in 4% formaldehyde (FA) solution and permeabilized with 0.2% Triton X-100, followed by blocking for 1 h in 10% goat serum. Primary anti-SLFN5 #111/1, dialyzed against azide-free PBS, or anti-V5 (mouse monoclonal; Thermo Fisher Scientific^TM) were diluted 1:500, respectively. Alexa Fluor 488 goat anti-mouse IgG (1:1000, Life Technologies) was used as a secondary antibody. DNA was counterstained with Hoechst 33342 (1:1000, Life Technologies^TM). Images were acquired and analyzed using a Zeiss Cell Observer.Z1 system equipped with an AxioCam MRm camera or Axioskop equipped with an AxioCam MRm camera. Image analysis was performed with ZEN lite 2011 software.

Vit G., Hirth A., Neugebauer N., Kraft B.N., Sigismondo G., Cazzola A., Tessmer C., Duro J., Krijgsveld J., Hofmann I., Berger M., Klüter H., Niehrs C., Nilsson J, & Krämer A. (2022). Human SLFN5 and its Xenopus Laevis ortholog regulate entry into mitosis and oocyte meiotic resumption. Cell Death Discovery, 8, 484.

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Monocyte-CSC Fusion Kinetics

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Monocytes (2x10⁴ cells/well) were labeled with DID and cultured on a 1μ-Slide 8 Well ibiTreat plate (ibidi, Germany). H460-CSCs (2x10³ cells) were labeled with DIO, added to DID-monocytes, and plates placed on a Cell Observer® Z1 system (ZEISS) with standard culture conditions. Fifteen positions were selected randomly to take photos at an initial 5 min, and then in a 15 min interval during 72 h. Images were qualitatively analyzed using AxioVision LE 4.8.2.0 software (ZEISS).
A quantitative analysis of populations’ dynamics and fusion kinetics was performed by seeding 1.8 × 10⁵ DID-monocytes from three HVs and 2 × 10⁴ DIO-H460-CSC cells, in a treated 24-well clear-film-bottom black plate (Eppendorf). Photos were taken every 20 min for the first 24 h, and every 90 min after to complete 120 h of video recording. Three fields per well were counted for red (DID⁺-monocytes)/green (DIO⁺-tumor cells)/tangerine (DID⁺DIO⁺-hybrids) dots with ImageJ.

Aguirre L.A., Montalbán-Hernández K., Avendaño-Ortiz J., Marín E., Lozano R., Toledano V., Sánchez-Maroto L., Terrón V., Valentín J., Pulido E., Casalvilla J.C., Rubio C., Diekhorst L., Laso-García F., del Fresno C., Collazo-Lorduy A., Jiménez-Munarriz B., Gómez-Campelo P., Llanos-González E., Fernández-Velasco M., Rodríguez-Antolín C., Pérez de Diego R., Cantero-Cid R., Hernádez-Jimenez E., Álvarez E., Rosas R., dies López-Ayllón B., de Castro J., Wculek S.K., Cubillos-Zapata C., Ibáñez de Cáceres I., Díaz-Agero P., Gutiérrez Fernández M., Paz de Miguel M., Sancho D., Schulte L., Perona R., Belda-Iniesta C., Boscá L, & López-Collazo E. (2020). Tumor stem cells fuse with monocytes to form highly invasive tumor-hybrid cells. Oncoimmunology, 9(1), 1773204.

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