Itq 1100

Manufactured by Thermo Fisher Scientific

Sourced in United States

The ITQ 1100 is a quadrupole ion trap mass spectrometer designed for analytical applications. It provides high-performance mass analysis and data acquisition capabilities. The device utilizes a quadrupole ion trap to capture, store, and analyze ions, enabling the detection and identification of various chemical species.

Automatically generated - may contain errors

Lab products found in correlation

Trace gc ultra, by Thermo Fisher Scientific (5 mentions) Agilent 1200, by Agilent Technologies (1 mentions) Hpx 87h, by Aminex (1 mentions) Db 5 column, by Agilent Technologies (1 mentions) Aviii hd 600 mhz spectrometer, by Bruker (1 mentions) Spme fiber, by Merck Group (1 mentions) Gas chromatograph mass spectrometer, by Thermo Fisher Scientific (1 mentions) Db 5ms ui column, by Agilent Technologies (1 mentions)

11 protocols using itq 1100

Analytical Methods for Biomass Conversion

Check if the same lab product or an alternative is used in the 5 most similar protocols

HPLC (Waters 2489
equipped with Waters 1525 binary HPLC pump and UV detector at 284
nm) was utilized to analyze the yields of HMF and furfural. The column
was thermostated at 30 °C. The eluent CH₃OH/H₂O (0.2:0.8, v/v) was run at a flow rate of 0.8 mL min^–1.
The carbohydrates (Glc, Fru, and furfural)
and organic acid content were analyzed using a liquid chromatography
system Agilent 1200 equipped with Aminex HPX-87H as a stationary phase
and a refractive index detector. The column was thermostated at 65
°C. The eluent (0.05 M H₂SO₄) was run at
0.55 mL min^–1 flow rate. The amount of Cr³⁺ ions in a water solution collected after the reaction was quantified
by ICP-AES (IRIS Intrepid II XSP). HMF and side products were also
quantified using MS (Thermo Fisher ITQ-1100). The HMF yield from Glc
was calculated as

Liang F., Chen D., Liu H., Liu W., Xian M, & Feng D. (2019). One-Pot Synthesis of 5-Hydroxymethylfurfural from Glucose by Brønsted Acid-Free Bifunctional Porous Coordination Polymers in Water. ACS Omega, 4(5), 9316-9323.

+ Open protocol

+ Expand

Oxygen Incorporation in Diol Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols

An experiment using labeled water (H₂O¹⁸) was conducted to confirm the incorporation of oxygen into the reaction product diol from the water molecule. For this, 50 µg Yleh protein was incubated with 5 mM 1,2-EO at 30 °C for 1 h in 1 ml 50 mM Tris-sulphate buffer, pH 8.0, and an equal volume of labeled water (H₂O¹⁸). Trace-Ultra GC (Thermo Fisher Scientific, USA) was used for GC-MS analysis. The sample was then centrifuged and extracted with ethyl acetate (1:1 v/v). It was further concentrated by drying and injected on a DB-5 column (30 m x 0.25 mm x 0.25 mm, Agilent Technologies, USA) connected to GC-MS and a mass detector (ITQ 1100, Thermo Fisher Scientific, USA) with helium as a carrier gas. The program used for analysis was as follows: initial temperature 60 °C, 15 °C/min up to 160 °C held for 30 s and 3 °C/min up to 200 °C and held for 1 min. The reaction product peaks were identified by m/z values and retention time using NIST Mass Spectral Library (NIST 05).

Godase V.P., Kumar V.R, & Kumar A.R. (2023). Potential of Y. lipolytica epoxide hydrolase for efficient production of enantiopure (R)-1,2-octanediol. AMB Express, 13, 77.

+ Open protocol

+ Expand

Characterization of Novel Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Melting points (mp) were determined on a WRX-4 electrothermal melting point apparatus (Shanghai, China) and were uncorrected. The ¹H NMR and ¹³C NMR spectra were recorded on a Bruker AV III HD 600 MHz spectrometer using CDCl₃ or DMSO-d₆ as solvent. Chemical shifts were expressed relative to tetramethylsilane (TMS) used as an internal standard and were reported as δ (ppm). The mass spectra were taken on Thermo Scientific ITQ 1100 instrument (Thermo Fisher Scientific, Waltham, MA, USA) with an EIS source and an ion trap analyzer in the positive ion mode. All the reactions were monitored by thin-layer chromatography (TLC) on Silica gel GF-254 plates and the products were separated by flash column chromatography on Silica gel H (Qingdao Haiyang Chemical, Qingdao, China).

Wei Z., Tan J., Cui X., Zhou M., Huang Y., Zang N., Chen Z, & Wei W. (2020). Design, Synthesis and Bioactive Evaluation of Oxime Derivatives of Dehydrocholic Acid as Anti-Hepatitis B Virus Agents. Molecules, 25(15), 3359.

+ Open protocol

+ Expand

GC-MS Analysis of Volatile Compounds

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The GC–MS analyses of the samples were performed using a Trace GC Ultra gas spectrometer coupled to an ITQ 1100 mass spectrometer from ThermoFisher Scientific (Waltham, MA, USA) following the procedure described in our previous publications. An SPME (solid-phase micro-extraction) fiber with an absorbent (50/30 μm Divinylbenzene/Carboxene/Polydimethylsiloxane (DVB/CAR/PDMS), Stableflex (2 cm) 24 Ga (Sigma Aldrich, Poznań, Poland), was placed in the measurement chamber for 30 min together with the material emitting volatile compounds and then transferred to a GC injector for 5 min to desorb VOCs. The chromatographic analysis was carried out with the use of a Zebron ZB-5Msplus Capillary GC 30 m × 0.25 mm × 0.25 um column. The compounds were identified using the Wiley library. Compounds with a 75% identity level were selected [23 (link)].

Rusinek R., Żytek A., Stasiak M., Wiącek J, & Gancarz M. (2024). Application of MOX Sensors to Determine the Emission of Volatile Compounds in Corn Groats as a Function of Vertical Pressure in the Silo and Moisture Content of the Bed. Sensors (Basel, Switzerland), 24(7), 2187.

+ Open protocol

+ Expand

Transesterification and GC-MS Analysis of Fatty Acids

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mixture of fatty acid methyl esters was obtained by transesterification of lipids with methanol in the presence of catalysts^{13 (link)}. Briefly, crude oil suspended in n-hexane (HPLC grade, Sigma-Aldrich) was evaporated and 0.5 M KOH-methanol (Sigma-Aldrich) was added and hydrolyzed at the temperature of 80 °C. Esterification was carried out by adding 10% BF₃ in methanol (for GC derivatization, Fluka). The reaction was performed at 100 °C for 20 min. Next, 99% n-hexane (HPLC grade, Sigma-Aldrich) and a saturated NaCl solution (Sigma-Aldrich, Supelco) were successively added. Separation of methyl esters and determination of fatty acids was performed on a Trace GC Ultra gas chromatograph coupled with ion trap mass spectrometer ITQ 1100 (GC/MS) from Thermo Scientific using a Rtx-2330 column, with a length of 105 m, inner diameter of 0.25 mm, and film thickness of 0.25 μm. Helium at a flow rate of 2.4 ml/min was the carrier gas. To determine the fatty acid composition, separation of the mixture of standard solutions − 37 Component FAME Mix solutions (Sigma-Aldrich, Supelco) was carried out.

Piasecka A., Nawrocka A., Wiącek D, & Krzemińska I. (2020). Agro-industrial by-product in photoheterotrophic and mixotrophic culture of Tetradesmus obliquus: Production of ω3 and ω6 essential fatty acids with biotechnological importance. Scientific Reports, 10, 6411.

+ Open protocol

+ Expand

Volatile Organic Compounds from Bacterial Strain DZSY21

Check if the same lab product or an alternative is used in the 5 most similar protocols

The VOCs produced by strain DZSY21 were collected using a 2-cm divinyl benzene/carboxen/PDMS (DCP, 50/30 μm) solid phase microextraction (SPME) fiber (Supelco, Bellefonta, PA, USA). Cell suspensions (20 μL, 10 8 CFU/mL) of strain DZSY21 were added into LB agar medium in a 100-mL vial covered with several layers of parafilm and incubated at 28 °C for 7 days without agitation. Uninoculated LB medium was used as the control. SPME fiber was then inserted into the vials and incubated at 50 °C for 30 min. After that, the SPME fiber was inserted into the GC-MS injector (Thermo Scientific ITQ1100, Waltham, MA, USA) and desorbed at 220 °C for 5 min (Xie et al. 2018; Jun et al. 2012) . The GC-MS program was 35 °C maintained for 3 min, then increased to 180 °C at 10 °C/min, further increased to 240 °C at 10 °C/min, and held for 5 min. The mass spectrometer was operated in the electron ionization mode at 70 eV with a source temperature of 220 °C, with scans from m/z 50 to 500. Mass spectral data were compared with data in the NIST/EPA/NIH Mass Spectrum Library (Xie et al. 2018) . Each treatment had three replications, and the assay was repeated three times.

Xie S., Liu J., Gu S., Chen X., Jiang H., & Ding T. (2020). Antifungal activity of volatile compounds produced by endophytic Bacillus subtilis DZSY21 against Curvularia lunata. Annals of Microbiology, 70(1).

+ Open protocol

+ Expand

HCB-Contaminated Soil Remediation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The 200g treated real HCB-contaminated soil sample were added to the wild-mouth bottle and mixed with a certain proportion of deionized water to form slurry. 0.8 g acetic acid and 0.2g iron nanoparticles were added, respectively. Then the pH of slurry was adjusted using NaOH and H 2 SO 4 solution and the initial pH was measured with a pH meter (PHSJ-4A, Shanghai ShengCi Instrument Company, China). The bottle was sealed, with N 2 gas sparged, and was incubated under the set temperature in the biochemical incubator for 23 days. At the end of each experiment, the supernatant of the mixture was separated from the solid residue using suction lter device. The quanti cation and structural con rmation of all four chlorobenzenes in the mud phase were analyzed using Gas Chromatograph-Mass Spectrometer (Thermo Fisher ITQ 1100). Each experiment was conducted in triplicate.

Wang Q. (2023). Degradation behaviors and accumulative effects of coexisting chlorobenzene congeners on the dechlorination of hexachlorobenzene in soil by nanoscale zero-valent iron. Environmental geochemistry and health, 45(6).

+ Open protocol

+ Expand

GC-MS Quantification of Essential Oils

Check if the same lab product or an alternative is used in the 5 most similar protocols

Essential oil was diluted 100 times using n-hexane to achieve a 1 mL volume, and then 10 μL C12 and C19 was added to the diluted oil as an internal standard mixture solution (1 mg/mL in toluene). Samples thus prepared were subjected to quantitative GC-MS determination, as described by Kowalski and Wawrzykowski (2009) . The GC-MS analysis was performed on a TRACE GC Ultra Thermo chromatograph linked to a Thermo ITQ 1100 mass spectrometer using a DB-5 capillary column. The parameters set for the DB-5 column were 30 m × 0.32 mm i.d., 0.25 µm film thickness, column, oven temperature 50°C for 1 min, then 3°C/min to 200°C for 10 min. Helium was used as the carrier gas at a flow rate of 1 mL/min. The injector and detector temperatures were 200°C. A mass selective detector was operated in electron impact mode with ionization energy of 70 eV, a scan time of 0.5 s, and a mass range of 40-870 AMU. The qualitative analysis was based on MS spectra, which were compared with spectra from the NIST library (National Institute of Standards and Technologies, Mass Spectra Libraries).

Guz L., Wawrzykowski J, & Adaszek Ł. (2021). Anti-babesial potential and chemical composition of essential oil from yarrow Achillea millefolium. Polish journal of veterinary sciences, 24(1).

+ Open protocol

+ Expand

GC-MS/MS Quantification of Analytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

GC-MS/MS analysis were carried out using a Trace GC Ultra, Thermo Scientific linked to an ion trap mass spectrometer (ITQ1100, Thermo Scientific) operating in splitless mode. Compounds were separated on an Agilent DB-5MS UI column (30 m Â 0.25 mm, 0.25-mm film thickness) using helium as the carrier gas (99.996% purity) at a flow rate of 1.3 ml/min. The injector and transfer line were set at 250 C and 300 C respectively, the source at 250 C. The column was held at 95 C for 6 min after injection and then programmed at 12 C/min to 320 C and held for 4 min. The mass spectrometer was operated in the electron ionization mode (EI, 70 eV) and analytes were detected using MS/MS mode. Analyte precursor and fragment ions and their associated IS used for quantitation are reported in Table S2. GC-MS/MS spectra were analysed on Xcalibur v1.2 software (Thermoquest-Finningan). Concentrations were determined using a least-square linear regression analysis of the peak area ratio (analyte to IS) versus the analyte concentration using a matrix-matched calibration curve.

Lentola A., David A., Abdul-Sada A., Tapparo A., Goulson D., & Hill E. (2017). Ornamental plants on sale to the public are a significant source of pesticide residues with implications for the health of pollinating insects. Environmental Pollution, 228, 297-304.

+ Open protocol

+ Expand

GC-MS Analysis of Lactones

Check if the same lab product or an alternative is used in the 5 most similar protocols

The analysis was carried out using GC-electron ionization mass spectrometry (GC-EI/MS; GC: TRACE GC Ultra, EI/MS: ITQ1100, Thermo Fisher Scientific, Waltham, MA) equipped with a capillary column (InterCap FFAP, 20 m× 0.18 mm i.d., 0.18 μm thickness, GL Sciences Ltd., Tokyo, Japan) . The column temperature was programmed to remain at 60℃ for 6 min, increase to 250℃ at 5℃ min -1 , and hold for 20 min. The injector temperature was set at 250℃. The flow rate of the carrier gas (helium) was 0.5 mL min -1 . The split ratio was 50:1 (v/v) and the injection volume was 1 μL. Electron impact spectra were acquired at 70 eV. Mass spectra were collected from m/z 20 to 300. The target compounds were detected at m/z＝85 for all γ-lactones and at m/z＝71 and 99 for δ-lactones, with the exception of δ-hexalactone (δ-C6) . The δ-C6 species was detected at m/z＝42. All analyses were carried out in triplicate and the mean values are presented.

Obi J., Yoshinaga K., Tago A., Nagai T., Yoshida A., Beppu F, & Gotoh N. (2018). Simple Quantification of Lactones in Milk Fat by Solvent Extraction Using Gas Chromatography-Mass Spectrometry. Journal of oleo science, 67(8).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!