T aoc kit

The antioxidant molecules are able to reduce Fe³⁺ to Fe²⁺, forming a stable complex with phenanthroline, whose antioxidant capacity can be evaluated by colorimetry. A T‐AOC kit (Nanjing Jiancheng Bioengineering Institute) was used to determine the total antioxidant capacity of C3G and VC, and the experiment was performed in accordance with the manufacturer's instructions. The detection wavelength was 520 nm using an ELISA microplate reader, and one T‐AOC unit (U) was defined as a 0.01 increase in absorbance per milliliter of the reaction system of the sample to be tested at 37°C per minute.

The standard substances isoquercitrin, orientin, and luteolin (all purity ≥ 98%) were purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China). isoquercitrin (purity ≥ 98%) was provided by Wuhan Chemfaces Bio-Technology Co., Ltd. (Wuhan, China), and orientin (purity ≥ 98%) was purchased from Shanghai yuanye Bio-Technology Co., Ltd. (Shanghai, China) in animal experiments. Quercetin (purity ≥ 97%), 2,2-diphenyl-1-pi-Crylhydrazyl (DPPH), 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-tris (2-pyridyl)-S-triazine (TPTZ), 2,2′-azobis (2-methylpropionamidine) dihydro-chloride (AAPH), fluorescein sodium, 6-hidroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), α-amylase (500 U/g), pepsin (≥2500 U/mg), trypsin (≥3000 U/mg), and formic acid (HPLC gradient grade) were purchased from the Aladdin Reagent Co., Ltd. (Shanghai, China). In addition, bile salt was obtained from Beijing Solarbio Technology Co., Ltd. (Beijing, China). Ultrapure water was purchased from Watsons (Hongkong, China). Acetonitrile (HPLC gradient grade) was provided by Thermo Fisher Scientific (Waltham, MA, USA). T-AOC kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China) and Beyotime Biotechnology (Shanghai, China).