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Caki1

Manufactured by CLS Cell Lines Service
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Sourced in Germany

The Caki1 is a cell line derived from a human renal cell carcinoma. It is a widely used model for the study of renal cell carcinoma.

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Lab products found in correlation

Venor gem classic, by Minerva Biolabs (1 mentions) Powerplex 21 system, by Promega (1 mentions) Rpmi 1640 medium, by Lonza (1 mentions) L glutamine, by Lonza (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Mccoy s 5a medium, by Merck Group (1 mentions)

2 protocols using caki1

1

Cell Line Cultivation and Authentication

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A498, 786-O, and Caki1 were purchased from CLS Cell Lines Service (Eppelheim, Germany). A498 cells were cultivated in EMEM (Lonza) supplemented with 10% FBS (Merck) and 2 mM l-glutamine (Lonza). 786-O cells were cultivated in RPMI 1640 medium (Lonza) supplemented with 10% FBS and 2 mM l-glutamine. Cell lines were routinely tested for mycoplasma infection using a PCR-based test (Venor® GeM Classic, Minerva Biolabs GmbH), and authentication of cell lines was performed using the PowerPlex® 21 System (Promega) according to the manufacturer’s protocol.
Reustle A., Di Marco M., Meyerhoff C., Nelde A., Walz J.S., Winter S., Kandabarau S., Büttner F., Haag M., Backert L., Kowalewski D.J., Rausch S., Hennenlotter J., Stühler V., Scharpf M., Fend F., Stenzl A., Rammensee H.G., Bedke J., Stevanović S., Schwab M, & Schaeffeler E. (2020). Integrative -omics and HLA-ligandomics analysis to identify novel drug targets for ccRCC immunotherapy. Genome Medicine, 12, 32.
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2

Culturing Clear Cell Renal Cell Carcinoma

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Human ccRCC cell line Caki-1 was purchased in CLS (RRID: CVCL_0234, CLS Cell Lines Service GmbH, Eppelheim, Germany), which was derived from a male ccRCC patient. Cells were cultured with proliferation media, McCoy’s 5A Medium (M9309, Sigma-Aldrich, Saint Louis, MO, USA) with 10% FBS and 1% Penicillin/Streptomycin supplemented media, 37°C.
Li X., Kim W., Juszczak K., Arif M., Sato Y., Kume H., Ogawa S., Turkez H., Boren J., Nielsen J., Uhlen M., Zhang C, & Mardinoglu A. (2021). Stratification of patients with clear cell renal cell carcinoma to facilitate drug repositioning. iScience, 24(7), 102722.
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