Pgems t easy vector system kit

Manufactured by Promega

Sourced in France

The PGEMs-T Easy Vector System kit is a cloning vector system designed for the efficient cloning of PCR products. It provides a linearized plasmid with 3' terminal thymidine (T) overhangs, enabling direct insertion of PCR products generated by thermostable DNA polymerases that leave 3' A-overhangs. The kit includes the pGEM-T Easy Vector, a high-copy-number plasmid with multiple cloning sites, and necessary reagents for the ligation and transformation steps.

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Lab products found in correlation

Truseq dna kit, by Illumina (1 mentions) Miseq system, by Illumina (1 mentions)

Close spelling variants of this product

PGEM-T Easy Vector Systems Kit PGEM®-T Easy Vector System kit PGEM-T Easy Vector System PGEM-T easy vector kit PGEM-T Easy vector PGEM-T Easy system PGEM® Easy Vector System PGEM-T Easy kit PGEM-T vector system PGEM-T vector kit

2 protocols using pgems t easy vector system kit

Validating maize nifH gene expression

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To corroborate T-RFLP data, qPCR products from DNA and cDNA samples of maize line W85 were cloned and sequenced, using the polF/polR primer set in 50 μl containing ~50 ng of purified qPCR products. PCR was carried out as described above. The PCR products were purified and cloned in the pGEMs-T Easy Vector System kit (Promega, Charbonnières, France) and positive clones were sequenced (Biofidal, Vaulx-en-Velin, France). Nucleotide sequences were analyzed using the SeaView multiplatform graphical user interface45 (link) (available at http://pbil.univ-lyon1.fr/) using MUSCLE46 (link) (default parameters), and phylogenetic trees inferred using PhyML46 (link) (version 3.0) with a GTR model of nucleotide substitution47 (link). Reference nifH genes were retrieved using BLASTP48 (link) at the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov).

Bouffaud M.L., Renoud S., Moënne-Loccoz Y, & Muller D. (2016). Is plant evolutionary history impacting recruitment of diazotrophs and nifH expression in the rhizosphere?. Scientific Reports, 6, 21690.

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Cloning and Illumina Sequencing of PCR Product

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The PCR product was purified and cloned into pGEMs-T Easy Vector System kit (Promega, France), and the positive clone was sequenced (Biofidal, Vaulx-en-Velin, France). According to the requirements of Illumina library preparation scheme, sequencing samples were prepared by TruSeq DNA kit, then applied to Illumina Miseq system, sequenced by Reagent Kit v2 2×250 bp.

Tian Y., Ma X., Li Y., Cheng C., An D, & Ge F. (2021). Exploring the structural changes in nitrogen-fixing microorganisms of rhizosheath during the growth of Stipagrostis pennata in the desert. Bioscience Reports, 41(4), BSR20201679.

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