Cyclin b1 12231

The MEK1/2 inhibitor U0126 (V1121) was purchased from Promega. Trametinib (MEK1/2 inhibitor, HY-10999), Stattic (STAT3 inhibitor, HY-13818), and ruxolitinib (JAK1/2 inhibitor, HY-50856) were obtained from MedChemExpress (MCE, China). Blasticidin (BSD, R21001) and EGF (PHG0311) were obtained from Thermo Fisher Scientific. OSM (10452-HNAH) and human recombinant IL-6 (10395-HNAE) were purchased from Sino Biological Inc. (Beijing, China). X-gal (0428-1 G) was purchased from Maygene Inc. (Guangzhou, China).
Antibodies against STAT3 (9139 s), p-STAT3 Y705 (9145 s), p-p44/42 MAPK (ERK1/2) (4370 s), p44/42 MAPK (ERK1/2) (4695 s), cyclin E2 (4656), and cyclin B1 (12231) were obtained from Cell Signaling Technology (Shanghai, China). pERK (E-4) (sc-7383) and p-ELK-1 (B-4) (sc-8406) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against GAPDH (60004-1-Ig), α-tubulin (66031-1-Ig), Flag (DDDDK tag) (20543-1-AP), SOCS3 (14025-1-AP), p21 (10355-1-AP), and cyclin D1 (60186-1-Ig) were purchased from Proteintech. Anti-ELK1 antibody (ab32106) was purchased from Abcam (Shanghai, China).

We lysed the cell pellet using a mixture of RIPA lysis buffer and protease inhibitor on ice. The protein quantification was performed using bicinchoninic acid assay, followed by electrophoresis and transfer as previously described [21 ]. The antibodies (human specific) for immunoblotting namely cyclin B1 (12231), CDK1(9116), p-CDC25C (Ser216, 4901), p-Histone H3 (Ser10, 3377S), PARP (9542), caspase 3 (9662S), caspase 9 (9502) and Bcl-2 (15071S) were supplied by Cell signaling Technology Company (Danvers, MA, USA). While antibodies for anti-p-CDK1 (Tyr15, sc-136014) and CDC25C (sc-13138) were from Santa Cruz Biotechnology Company (Dallas, TX, USA), β-actin (66009-1) and GAPDH (60004-1) were purchased from Proteintech Company (Shanghai, PR China).