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Fetal bovine serum s001 01

Manufactured by Welgene

Fetal bovine serum (FBS) (S001-01) is a cell culture supplement derived from the blood of bovine fetuses. It provides a source of growth factors, hormones, and other nutrients essential for the in vitro cultivation of a wide range of cell types.

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2 protocols using fetal bovine serum s001 01

1

Detailed CK2, HIF-1α, STAT-3/5 Inhibition Protocol

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CX-4945 was purchased from Cayman Chemical (Ann Arbor, MI, USA). RPMI 1640 (LM-011-60), fetal bovine serum (FBS) (S001-01), and cocktail of penicillin/streptomycin cocktail (LS202-02) were obtained from Welgene, Inc. (Daegu, Republic of Korea). Control siRNA (cat. no. sc-37007), CK2α siRNA (cat. no. sc-29918), HIF-1α siRNA (cat. no. sc-35561), STAT-3 siRNA (cat. no. sc-29493), and STAT-5 siRNA (cat. no. sc-29495) were purchased from Santa Cruz Biotechnology (Delaware, CA, USA). A detailed list of antibodies used in this study is included in Supplementary Table S1. Western BrightTM Enhanced chemiluminescence (ECL, cat. no. K-12045-D20) was purchased from Advansta Corporation (San Jose, CA, USA). All the plasticwares used for cell culture were obtained from SPL Life Sciences (Gyeonggi-do, Korea).
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2

Cell Culture Reagents and Chemical Compounds

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Roswell Park Memorial Institute Medium (RPMI)-1640 (LM011-01), Dulbecco’s Modified Eagles Medium (DMEM; LM001-05), fetal bovine serum (FBS; S001-01), penicillin-streptomycin (LS202-02) and phosphate-buffered saline (PBS) were purchased from Welgene (Daegu, Republic of Korea). PHA-665752 (A2307), SU11274 (A2678), JNJ38877605 (943540-75-8), and INCB28060 (A8448) were purchased from ApexBio Technology (Seoul, Republic of Korea). Doxorubicin (BML-GR319) was purchased from Enzo Life Science (Seoul, Republic of Korea). Antibodies and their dilution used in this study are mentioned in Table S1 as Supplementary File. Enzyme-linked chemiluminescence (ECL) Western detection reagents were bought from Thermo Scientific (Waltham, MA, USA). Cell culture plastic wares were purchased from SPL Life Sciences (Gyeonggi-do, Republic of Korea).
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