Rhodamine b rhb

Methacrylate gelatin (GelMA, crosslinking degree ≈90%) was provided by Aladdin. Sodium alginate (low viscosity) and agarose were bought from Alfa Aesar. Matrigel was obtained from Corning. 2‐hydroxy‐2‐methylpropiophenone (HMPP), calcium chloride, and rhodamine B (RhB) were purchased from Sigma‐Aldrich. Fresh porcine skins were bought from the market. Heparin, erythropoietin, cyclophosphamide, cyclophosphamide, and ketoconazole (KCZ) were provided by Drum Tower Hospital. FITC‐BSA was from Zhongkechenyu Biotech. SYTO was offered by KeyGEN Biotech. Sabouraud dextrose agar medium (SDA), nutrient agar medium (NA), and Luria–Bertani culture medium (LB) were purchased from Hopebio Co., Ltd. Deionized water (resistivity: 18.2 MΩ cm⁻¹) was purified by a Milli‐Q water purification system (Millipore). B. subtilis 3610 and C. albicans SC5314 were received from BeNa Biotech Co., Ltd. Male BALB/c mice (about 8 weeks and 20 g) were obtained from Drum Tower Hospital. Animals were treated in strict accordance with the Beijing Administration Rule of Animals in China and had received approval from Animal Investigation Ethics Committee of Drum Tower Hospital.

Rhodamine B (RhB; Sigma-Aldrich, St. Louis, MO, USA), poly-(methyl methacrylate) (PMMA; MW: 350,000; Alfa Aesar (China), Shanghai, China), anisole (Sigma-Aldrich, St. Louis, Mo, USA), cysteamine (Sigma-Aldrich, St. Louis, MO, USA); Au-Ag alloy nanoshuttle solution (Nanjing NANOEAST Biotech Co., Ltd., Nanjing, China); 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI; Sigma-Aldrich, St. Louis, MO, USA), paraformaldehyde (PFA; Alfa Aesar (China), Shanghai, China). Ethyl alcohol, dimethylsulfoxide (DMSO), H₂SO₄, NaCl, KCl, Na₂HPO₄·12H₂O, and KH₂PO₄ were all analytical grade reagents (all from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China). Ultrapure water was prepared using a Milli-Q system. The synthesis process of Au-Ag NSs is mentioned in the supporting information.

The model drug reservoirs were formed by spreading solutions of fluorescent probes (Fluorescein Disodium (FD) (Alfa Aesar™, Ward Hill, MA, USA) or Rhodamine B (RhB) (Sigma Aldrich, Taufkirchen, Germany) over the surface of a gelatin stripe at areas corresponding to the prescribed radial positions of the reservoirs inside the capsule after the rolling. Next, 20 μL of a solution per reservoir was spread using a micropipette. The concentration of the solution was 25 µg/µL, corresponding to a content of 500 µg within each reservoir. Reservoirs R₀, R₁, R₂ and R₃ were formed separately or in pairs on the gelatin stripe at precalculated distances so that each reservoir covered a full turn in the capsule (cf. Appendix C). R₀ was located at the end of the stripe and it partially covered the internal surface of the capsule; therefore, it was in direct contact with the central cavity of the capsule after rolling. The R₁, R₂, and R₃ reservoirs were formed in such a way so that after rolling they are located on the 3rd, 6th and 8.5th turn, respectively; the total number of full turns was equal to 10. The coordinates of the boundaries with respect to the end of the gelatin strip, from which the rolling started, are given in Table 1.