Mg101

Each inhibitor was added 20 min before stimulation with LL-37 or CRAMP. BAPTA (inhibitor of intracellular calcium release); U-73122 (phospholipase C inhibitor); GF109203X (Protein kinase C inhibitor); SB203580 (p38-MAPK inhibitor), MG101 (calpain inhibitor); wortmannin (phosphoinositide 3-kinase inhibitor); Boc-MLF (FPR1-receptor inhibitor); WRW4 (FPR2-receptor inhibitor); and A438079 (P2X7 receptor inhibitor) were all obtained from Tocris (Bristol, UK). Pertussis toxin and cholera toxin (inhibitors of G-protein signaling) and Tirofiban (GPIIb/IIIa inhibitor) were from Sigma. Abciximab was from Elly Lilly (USA). Dasatinib (inhibitor of Src-family kinases), R406 (Syk Inhibitor), and Stattic (STAT3-Inhibitor) were from Selleckchem (Houston, USA). Anti-mouse GPVI antibody was from Emfret (clone JAQ1, Cat No. M011-0). The inhibitory monoclonal antibody HGP5C4 directed against human GPVI and the respective isotype control antibody RmC7H8 were generated by immunization of Lou/C rats with an adenovirally expressed human GPVI-Fc fusion protein. The latter represents a soluble form of GPVI with the extracellular domain of human GPVI fused to the human Fc domain. 4C9 and 5C4 monoclonal antibodies (immunoglobulin G1 subtype) specifically bound to GPVI-Fc but not control Fc^{65 (link)}. RmC7H8, raised in rats against an irrelevant human antigen, served as control monoclonal antibody (mAb).

Rabbit phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP™ (#4511) and β-Actin (13E5) antibodies were from Cell Signaling Technology, Frankfurt am Main, Germany. HIF-1α clone H1α67, HIF-1α clone EP1215Y, anti-mouse and -rabbit horseradish peroxidase-conjugated secondary antibodies were from Merck Millipore, Darmstadt, Germany. Src-Inhibitor PP2 was purchased from Sigma-Aldrich (#P0042), Darmstadt, Germany. p38 MAPK inhibitor (SB203580), MG132 and MG101 were from Tocris, Wiesbaden-Nordenstadt, Germany. Bortezomib and Epoxomicin were from Calbiochem. All other chemicals were from Sigma-Aldrich, Darmstadt, Germany.