Log In Sign up
The largest database of trusted experimental protocols

35 mm dishes

Manufactured by STEMCELL
Visit Supplier

35 mm dishes are a type of laboratory culture vessel used to grow and maintain cells in a controlled environment. These dishes provide a flat, circular surface area for cell attachment and growth. They are typically made of treated polystyrene and have a diameter of 35 millimeters.

Automatically generated - may contain errors

Lab products found in correlation

Methocult h4034 optimum, by STEMCELL (1 mentions) Methocult gf m3434, by STEMCELL (1 mentions)

4 protocols using 35 mm dishes

1

LED Array for Cell Illumination

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were illuminated using a custom-built 4×6 LED array26 (link) (~16 mW/cm2), measured using a Newport Power Meter held directly above LEDs, Model No. 1931-C). Six blue Rebel LEDs (450 nm, 48 Lumens @ 700 mA, Philips Lumileds) pre-mounted on a 10 mm square base (Luxeon Star LEDs, Ontario, Canada) were connected in series, and four of these LED series were connected in parallel and mounted on a printed circuit board (Radio Shack). The LED array was powered using an Arduino Uno microcontroller programmed to pulse illumination for 1 second at 0.067 Hz. Current was regulated using a BuckPuck AC Driver (700 mA, Luxeon Star LEDs). For all experiments except patterning, the LED array was held 8″ above the incubator shelf with illumination facing downward such that cells were illuminated from the top of the dish. For patterning experiments cells were plated in 35 mm dishes (StemCell Technologies) that were set atop of the upward-facing LED array. A photomask made by a 3D printer was placed between the upward-facing LED array and the bottom of the 35 mm culture dish. Plates containing cells incubated in the dark were wrapped in aluminum foil.
Polstein L.R, & Gersbach C.A. (2015). A light-inducible CRISPR/Cas9 system for control of endogenous gene activation. Nature chemical biology, 11(3), 198-200.
+ Open protocol
+ Expand
2

Hematopoietic Colony-Forming Unit Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
To assess hematopoietic colony forming unit (CFU) potential, 2 × 104 to 1 × 105 viable cells were plated in individual 35 mm dishes (StemCell Tech., Vancouver, CA; #27150) in 1.5 ml multi‐lineage CFU semi‐solid media (StemCell Technologies, Vancouver, CA; MethoCult H4034 Optimum) per dish. Duplicate dishes were generated for each condition. Colonies were scored after 14 days of culture at 37°C in normoxic, 5% CO2 conditions. CFU values were normalized to the number of cells plated (CFU per 104 cells plated).
Leung A., Zulick E., Skvir N., Vanuytsel K., Morrison T.A., Naing Z.H., Wang Z., Dai Y., Chui D.H., Steinberg M.H., Sherr D.H, & Murphy G.J. (2018). Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells. Stem Cells (Dayton, Ohio), 36(7), 1004-1019.
+ Open protocol
+ Expand
3

Bone Marrow Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bone marrow differentiation was assessed by using a methylcellulose-based colony-forming assay according to the manufacturer's description (StemCell Technologies, Vancouver, BC, Canada). Isolated bone marrow was separated for different lineages and counted. Bone marrow cells were mixed with 3.5 ml of semisolid methylcellulose-based medium (MethoCult GF M3434) (StemCell Technologies) containing Stem Cell Factor, IL-3, IL-6, and erythropoietin, and triplicate 1.1 ml aliquots of the cell-containing media was dispensed into 35-mm dishes (StemCell Technologies). Colony plates were cultured in incubators at 37 ?C, 5% CO2 and 95% humidity in the presence or absence of CO (250 p.p.m.). Colonies were counted at day 3, 6, 8, and 9 and cells were harvested for protein analysis.
Wegiel B., Hedblom A., Li M., Gallo D., Csizmadia E., Harris C., Nemeth Z., Zuckerbraun B.S., Soares M., Persson J.L, & Otterbein L.E. (2014). Heme oxygenase-1 derived carbon monoxide permits maturation of myeloid cells. Cell Death & Disease, 5(3), e1139-.
+ Open protocol
+ Expand
4

LED Array for Cell Illumination

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were illuminated using a custom-built 4×6 LED array26 (link) (~16 mW/cm2), measured using a Newport Power Meter held directly above LEDs, Model No. 1931-C). Six blue Rebel LEDs (450 nm, 48 Lumens @ 700 mA, Philips Lumileds) pre-mounted on a 10 mm square base (Luxeon Star LEDs, Ontario, Canada) were connected in series, and four of these LED series were connected in parallel and mounted on a printed circuit board (Radio Shack). The LED array was powered using an Arduino Uno microcontroller programmed to pulse illumination for 1 second at 0.067 Hz. Current was regulated using a BuckPuck AC Driver (700 mA, Luxeon Star LEDs). For all experiments except patterning, the LED array was held 8″ above the incubator shelf with illumination facing downward such that cells were illuminated from the top of the dish. For patterning experiments cells were plated in 35 mm dishes (StemCell Technologies) that were set atop of the upward-facing LED array. A photomask made by a 3D printer was placed between the upward-facing LED array and the bottom of the 35 mm culture dish. Plates containing cells incubated in the dark were wrapped in aluminum foil.
Polstein L.R, & Gersbach C.A. (2015). A light-inducible CRISPR/Cas9 system for control of endogenous gene activation. Nature chemical biology, 11(3), 198-200.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!