The 3D-printed hydroxyapatite scaffolds (3D-printed HA) were fabricated as described previously16 (link). First, calcium sulfate-based powder (Visijet PXL, 3D Systems, USA) was loaded into the powder-based three-dimensional printing machine (PROJET 160, 3D Systems, USA) to print the closed disc-shaped specimens with 5 mm in diameter and 2 mm in thickness without any designed pore channels in the specimen using a commercial liquid binder (Visijet PXL clear, 3D Systems, USA). Regarding the printing parameters, the layer thickness was set at 0.1 mm, and the disc sample was oriented to build the thickness in the Z direction. Next, the printed samples were phase transformed to hydroxyapatite using the dissolution–precipitation principle by immersing them in a 1 M disodium hydrogen phosphate solution (Sigma Aldrich, USA) at 100 °C for 24 h. Afterward, the scaffolds were cleaned with distilled water, oven-dried, and sterilized using an ethylene oxide sterilizer.
Projet 160
Manufactured by 3D Systems
Sourced in United States
The PROJET 160 is a compact 3D printer designed for professional use. It offers accurate and reliable part production with a build volume of 254 x 254 x 380 mm. The printer utilizes Digital Light Processing (DLP) technology to create high-quality, detailed parts. Key specifications include a layer thickness range of 25 to 100 microns and a XY resolution of 1600 x 1600 DPI.
Automatically generated - may contain errors
2 protocols using projet 160
1
3D-printed Hydroxyapatite Scaffold Fabrication
2
3D Printed Hydroxyapatite Granules Protocol
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3D printed hydroxyapatite was prepared as described in previous studies [13, [16] [17] .
Briefly, a calcium sulfate-based powder mixture (Visijet PXL, 3D Systems) was loaded into a powder-based, three-dimensional printing machine (Projet 160, 3D systems) to print cylindershaped granules that were 1.0 mm in both diameter and thickness. (BoneCeramic, 0.5-1 mm, Straumann., Basel, Switzerland), were purchased and used as control samples for comparison. Morphology was characterized by using a scanning electron microscope (JEOL JSM-7800F Prime). All the samples were gold-sputtered prior to observation.
Briefly, a calcium sulfate-based powder mixture (Visijet PXL, 3D Systems) was loaded into a powder-based, three-dimensional printing machine (Projet 160, 3D systems) to print cylindershaped granules that were 1.0 mm in both diameter and thickness. (BoneCeramic, 0.5-1 mm, Straumann., Basel, Switzerland), were purchased and used as control samples for comparison. Morphology was characterized by using a scanning electron microscope (JEOL JSM-7800F Prime). All the samples were gold-sputtered prior to observation.
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