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Allophycocyanin clone zet

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Allophycocyanin clone ZET is a fluorescent protein used in flow cytometry and other immunoassays. It is derived from a cyanobacterial light-harvesting protein and emits light in the far-red/near-infrared spectrum.

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Lab products found in correlation

Lsm 780 confocal microscope, by Zeiss (2 mentions) Alexa fluor 488 rabbit polyclonal, by Thermo Fisher Scientific (2 mentions) B220 pe ra3 6b2, by BioLegend (2 mentions)

2 protocols using allophycocyanin clone zet

1

Visualizing IFN-γ-Induced Cells in Spleens

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Spleens from immunized and control IL-27p28–eGFP transgenic mice were removed 6 h after polyIC administration and immediately fixed overnight using PLP buffer, as previously described (30 (link)). Spleens were flash frozen with liquid nitrogen in OCT and 20-μm tissue sections were obtained using a cryostat. Sections were left to dry for 30 min, blocked with 5% BSA for 1 h, and stained with anti-eGFP (Alexa Fluor 488 Rabbit polyclonal; Life Technologies), XCR1 (allophycocyanin clone ZET; BioLegend), and B220 (PE RA3-6B2; BioLegend) in 5% BSA. After washing extensively, sections were coverslipped with Fluoromount mounting medium and left to dry overnight. Slides were imaged on a Zeiss LSM 780 confocal microscope, and image analysis was performed with Fiji (31 (link)).
Kilgore A.M., Welsh S., Cheney E.E., Chitrakar A., Blain T.J., Kedl B.J., Hunter C.A., Pennock N.D, & Kedl R.M. (2018). IL-27p28 Production by XCR1+ Dendritic Cells and Monocytes Effectively Predicts Adjuvant-Elicited CD8+ T Cell Responses. ImmunoHorizons, 2(1), 1-11.
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2

Visualizing IFN-γ-Induced Cells in Spleens

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens from immunized and control IL-27p28–eGFP transgenic mice were removed 6 h after polyIC administration and immediately fixed overnight using PLP buffer, as previously described (30 (link)). Spleens were flash frozen with liquid nitrogen in OCT and 20-μm tissue sections were obtained using a cryostat. Sections were left to dry for 30 min, blocked with 5% BSA for 1 h, and stained with anti-eGFP (Alexa Fluor 488 Rabbit polyclonal; Life Technologies), XCR1 (allophycocyanin clone ZET; BioLegend), and B220 (PE RA3-6B2; BioLegend) in 5% BSA. After washing extensively, sections were coverslipped with Fluoromount mounting medium and left to dry overnight. Slides were imaged on a Zeiss LSM 780 confocal microscope, and image analysis was performed with Fiji (31 (link)).
Kilgore A.M., Welsh S., Cheney E.E., Chitrakar A., Blain T.J., Kedl B.J., Hunter C.A., Pennock N.D, & Kedl R.M. (2018). IL-27p28 Production by XCR1+ Dendritic Cells and Monocytes Effectively Predicts Adjuvant-Elicited CD8+ T Cell Responses. ImmunoHorizons, 2(1), 1-11.
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