Peripheral blood lymphocytes were isolated from heparinized peripheral blood samples using lymphocyte separation medium (Solarbio, Beijing, China) as per the manufacturer's protocol. The isolated peripheral blood lymphocytes were incubated with mouse anti-chicken CD3-FITC (Southern Biotech, Birmingham, AL) and mouse anti-chicken CD4-APC (Southern Biotech) or mouse anti-chicken CD8a-PE (Southern Biotech) at 37°C for 30 min, then washed twice with PBS to remove excess antibodies. The percentage of CD3+, CD4+, and CD8+ cells were analyzed with a FACSCalibur flow cytometer (Becton Dickinson and Company, Franklin Lakes, NJ). Cell phenotypic data were expressed as percentages of gated lymphocytes.
Mouse anti chicken cd4 apc
Manufactured by Southern Biotech
Sourced in United States
The Mouse anti-Chicken CD4-APC is a fluorescently labeled antibody that specifically binds to the CD4 protein expressed on the surface of chicken T helper cells. This product can be used for the identification and quantification of CD4-positive cells in chicken samples using flow cytometry.
Automatically generated - may contain errors
2 protocols using mouse anti chicken cd4 apc
1
Quantification of Chicken Lymphocyte Subsets
2
Chicken Immune Cell Phenotyping
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Samples from chickens in each group on each sample day were softly macerated, filtered through a 70-µm cell strainer (FALCON-Corning, NC, USA) into a centrifuge tube, and centrifuged at 352× g for 5 min. The cell pellets were suspended in 1 mL of PBS and counted; cells equivalent to 1 × 106/mL from each sample were transferred to a Falcon tube (FALCON-Corning) and stained with Mouse Anti-Chicken CD3-FITC (Fluorescein isothiocyanate) [20 ], Mouse Anti-Chicken CD4-APC (Allophycocyanin) [21 (link)], and Mouse Anti-Chicken CD8-PE (Phycoerythrin) [20 ] antibodies (SouthernBiotech, Birmingham, AL, USA). The cells were then washed with phosphate-buffered saline (PBS) (PH7.4, 0.01 M, 4°C) 3× and suspended in 500 L of PBS for CD3+, CD4+, and CD8+ phenotyping using a BD FACS (Becton, Dickinson fluorescence-activated cell sorting) Calibur flow cytometer (BD Biotec., San Diego, CA, USA). The generated data were analyzed using Cell Quest software (BD Biotec.).
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