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SNRPA is a protein complex that functions as a component of the small nuclear ribonucleoprotein (snRNP) involved in the splicing of pre-mRNA. It plays a critical role in the recognition and removal of introns during the processing of mRNA precursors.

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Lab products found in correlation

Sr140, by Santa Cruz Biotechnology (1 mentions) Sf3a2, by Santa Cruz Biotechnology (1 mentions) Sf3b1, by Santa Cruz Biotechnology (1 mentions) Tfiiic110, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Strap, by BD (1 mentions) Ddx17, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-SNRPA

2 protocols using snrpa

1

Western Blot Profiling of Spliceosomal Proteins

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Proteins were separated by 10% SDS-PAGE and probed with primary antibodies. Primary antibodies, at the dilution of 1:1000 for each, included: SNRPA (Santa Cruz, sc-376027); U2A’ (Santa Cruz, sc-393804); SR140 (Santa Cruz, sc-398718); HELIC2 (Santa Cruz, sc-393170); PRPF3 (Santa Cruz, sc-101130); SYF1 (Santa Cruz, sc-271037); SF3A3 (Santa Cruz, sc-393673); SF3A2 (Santa Cruz, sc-390444); SF3B1 (Santa Cruz, sc-514655); STRAP (BD Transduction Labs, #611346); DDX15 (Santa Cruz, sc-271686); TFIIIC110 (Santa Cruz, sc-81406); GAPDH (Cell Signaling, #2118), and CHERP (Santa Cruz, sc-100650). β-Actin (Sigma, A5316) was used at the dilution of 1:10,000.
Jin L., Chen Y., Crossman D.K., Datta A., Vu T., Mobley J.A., Basu M.K., Scarduzio M., Wang H., Chang C, & Datta P.K. (2020). STRAP regulates alternative splicing fidelity during lineage commitment of mouse embryonic stem cells. Nature Communications, 11, 5941.
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2

Monoclonal and Polyclonal Antibodies for RNAP II

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The monoclonal antibodies used in this study were 8WG16 (against POLR2A, the large subunit of RNAP II) from Biolegend (cat # 920102), SNRPC from Sigma (cat # SAB4200188), DHX9 from Abcam (cat # ab26271), SNRPA (cat # sc-101149), DDX5 (cat # sc-166167), DDX17 (cat # sc-86409) from Santa Cruz. The polyclonal antibodies were FUS (cat # A300–293A), EWSR1 (cat # A300–418A) and MATR3 (cat # A300–591A) from Bethyl, TAF15 from Novus (cat # NB100–567), DDX17 (cat # sc-86409) from Santa Cruz, and DHX9 (cat # ab 26271) from Abcam. Our rabbit polyclonal antibodies to THOC2, EIF4A3, and DDX39B have been described58 (link),59 (link).
Chi B., O’Connell J.D., Yamazaki T., Gangopadhyay J., Gygi S.P, & Reed R. (2018). Interactome analyses revealed that the U1 snRNP machinery overlaps extensively with the RNAP II machinery and contains multiple ALS/SMA-causative proteins. Scientific Reports, 8, 8755.
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