Nmri foxn1nu foxn1nu female mice

This study was conducted in compliance with the National Charter on the ethics of animal experimentation. Our experiments were approved by the “Comité National Institutionnel d'Éthique pour l’Animal de Laboratoire (CIEPAL)” (reference: NCE/2017–383). 10⁶ Daoy cells or 0.5 × 10⁶ HD-MB03 cells were injected, in medium containing 50% Corning® matrigel® matrix (VWR), subcutaneously, into the flank of 6-week-old NMRI-Foxn1nu/Foxn1nu female mice (Janvier Labs, Le Genest-Saint-Isle, France). Tumor volume was measured every other day with a caliper and calculated as follows: $V=\pi /6\times L\times l^2$ where L = tumor length; l = tumor width (in a 2D space, tangential to the mouse skin).
The experiment was stopped when the tumors of one experimental group reached a volume of 1000 mm³.

The study was carried out in strict accordance with the recommendations of the United Kingdom Coordinating Committee on Cancer Prevention Research’s Guidelines for the Welfare of Animals in Experimental Neoplasia. Our experiments were approved by the ‘Comité National Institutionnel d'Éthique Pour l'Animal de Laboratoire’ (CIEPAL, reference: NCE/2013-97). One million non-irradiated, P or X irradiated CAL33 cells (CR-MI group) were injected subcutaneously into the flank of 6-week-old NMRI-Foxn1^nu/Foxn1^nu female mice (Janvier Labs, Le Genest-Saint-Isle, France, n=10/group). The tumor volume (v=L × l² × 0.52) was determined following measurement with a caliper. When the tumors reached 1 cm³, the mice were killed and the tumors collected.