Standardized coagulation assays in citrated (3.2%)-plasma were as follows: prothrombin time (PT, Medirox Owren's PT, Medirox, Nyköping, Sweden), fibrinogen (Clauss method, HemosIL Q.F.A. Thrombin, Werfen, Barcelona, Spain), and D-dimer (HemosIL D-Dimer HS 500). Antibodies (classes IgG, IgM and IgA) against PF4-heparin complexes were tested both with a gel agglutination rapid assay (ID-PaGIA Heparin/PF4 Antibody Test, Bio-Rad Laboratories, USA) and an enzyme-linked immunosorbent assay (ELISA) (Asserachrom HPIA, Diagnostica Stago, France). ELISA (Asserachrom HPIA, Diagnostica Stago) results were reported as percentage of absorbance related to reference sample. The reference sample was within the manufacturer specifications (stated on package insert, dependent on reagent lot). Percentage result is calculated (sample absorbance/reference absorbance). The interpretation of positivity is as follows: < 30 % of reference; negative, 30-50%, borderline positive, >50% positive, and >100% strongly positive.These results are referred below as anti-PF4 antibodies [1] (link). Cardiolipin and beta-2-glycoprotein-I IgG antibodies were analyzed using Phadia 250 immuno-analyzer and reagents (Sweden).
Hemosil q f a thrombin
Manufactured by Werfen
Sourced in Spain
The HemosIL Q.F.A. Thrombin is a laboratory reagent used in the measurement of prothrombin time (PT) and activated partial thromboplastin time (aPTT) in clinical samples. It is a standardized thrombin preparation that serves as a component in coagulation testing procedures.
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Lab products found in correlation
Hemosil d dimer hs 500, by Werfen (1 mentions)
Asserachrom hpia, by Diagnostica Stago (1 mentions)
Technozym adamts13 activity elisa kit, by Technoclone (1 mentions)
Hemosil liquid antithrombin, by Werfen (1 mentions)
Hemosil calibration plasma, by Werfen (1 mentions)
Acl top 700, by Werfen (1 mentions)
Hemosil protein c, by Werfen (1 mentions)
2 protocols using hemosil q f a thrombin
1
Standardized Coagulation Assays and Antibody Testing
2
Coagulation Factors and Activity Assays
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Prothrombin time, fibrinogen and antithrombin were analysed on the ACL-TOP 750 (HemosIL ReadiPlasTin, HemosIL Q.F.A Thrombin, HemosIL Liquid Antithrombin, Werfen Barcelona, Spain). Von Willebrand antigen and activity were determined by immunoturbidimetric latex based assays (HemosIL von Willebrand Factor Antigen, HemosIL von Willebrand Ristocetin Cofactor assay; Werfen) on the ACL-TOP 700. Protein C was analyzed on ACL-TOP 700 (HemosIL Protein C, Werfen). ADAMTS13 was determined with the Technozym® ADAMTS-13 Activity ELISA kit (Technoclone, Wien, Austria). Von Willebrand antigen and activity are expressed in %. This is equivalent to IU/dL since these tests are calibrated against HemosIL Calibration Plasma (Werfen), which is traceable to the current International Standards.
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