C57bl 6j b6 wt mice

C57Bl/6J (B6) WT mice and Myc-GFP mice (B6;129-Myc^tm1Slek/J) were purchased from Charles River and Jackson Laboratories. PLZF-GFP (Zbtb16^GFP) mice were generated in the laboratory of D. Sant’Angelo as described previously^{51 (link)}. Mice were maintained under specific pathogen-free conditions. Standard food and water were given ad libitum. Where indicated, mice were fed high fat diet (HFD) (Research Diets; D12492) for 8 weeks. Mice were used at the foetal (embryonic day 14-18), neonatal (1-5 days old) or adult (6-12 weeks old) stages.
The E0771 murine breast adenocarcinoma cells, MC38 murine colon adenocarcinoma cells and B16.F10 melanoma cells were purchased from ATCC (Manassas, VA). Cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) with 10% (vol/vol) FCS (Gibco; Thermo Fisher Scientific) and 1% (vol/vol) penicillin/streptomycin (Sigma-Aldrich).