PP-containing small intestines were fixed in 10% formalin, routinely processed, and embedded in paraffin. Sections were stained with hematoxylin and eosin (H&E). For immunofluorescence staining, fixed, frozen small intestinal sections were rehydrated in PBS for 30 minutes and permeabilized in 0.2% Triton X-100/PBS for 10 minutes. The slides were blocked with 1% BSA/PBS for 1 hour at room temperature and incubated with Alexa Fluor 488 wheat germ agglutinin (WGA, Molecular Probes) and Alexa Fluor 568 Phalloidin (Molecular Probes) for 30 minutes. Sections were washed 3 times with PBS after each antibody incubation. After a 10-minute stain with DAPI, slides were mounted using Prolong Gold antifade (Molecular Probes). Sections were visualized using the BX51 microscope, DP72 camera, and DP2-BSW imaging software (Olympus America Inc.). WGA-positive luminal signals were quantified using Imaris software (Bitplane).
Alexa fluor 488 wheat germ agglutinin wga
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488 wheat germ agglutinin (WGA) is a fluorescent conjugate used to label and detect N-acetylglucosamine and sialic acid-containing glycoconjugates. It binds to the cell surface and can be used to visualize cellular structures.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 wheat germ agglutinin wga
1
Histological and Immunofluorescence Analysis of Small Intestine
2
Histological and Immunofluorescence Analysis of Small Intestine
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
PP-containing small intestines were fixed in 10% formalin, routinely processed, and embedded in paraffin. Sections were stained with hematoxylin and eosin (H&E). For immunofluorescence staining, fixed, frozen small intestinal sections were rehydrated in PBS for 30 minutes and permeabilized in 0.2% Triton X-100/PBS for 10 minutes. The slides were blocked with 1% BSA/PBS for 1 hour at room temperature and incubated with Alexa Fluor 488 wheat germ agglutinin (WGA, Molecular Probes) and Alexa Fluor 568 Phalloidin (Molecular Probes) for 30 minutes. Sections were washed 3 times with PBS after each antibody incubation. After a 10-minute stain with DAPI, slides were mounted using Prolong Gold antifade (Molecular Probes). Sections were visualized using the BX51 microscope, DP72 camera, and DP2-BSW imaging software (Olympus America Inc.). WGA-positive luminal signals were quantified using Imaris software (Bitplane).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!