A 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) kit was purchased from Promega (USA). MMP9, ERK1/2, and phosphorylated ERK1/2 (p ERK1/2) antibodies were purchased from Abcam (USA). An RNA extraction kit, reverse transcription kit, and quantitative polymerase chain reaction (qPCR) SuperMIX were purchased from TAKARA (Japan). Puerarin was purchased from Sigma (USA). Matrigel was purchased from BD (USA). The gene amplification instrument and fluorescence imaging system were obtained from Bio-RAD (USA). A microscopic imaging system (Leica, Germany) from Germany was also used.
Microscopic imaging system
Manufactured by Leica
Sourced in Germany
The Leica Microscopic Imaging System is a high-performance optical microscope designed for precise and detailed observation of microscopic samples. The system features advanced optics, high-resolution imaging capabilities, and user-friendly controls to facilitate detailed analysis and documentation of microscopic specimens.
Automatically generated - may contain errors
Lab products found in correlation
Matrigel, by BD (1 mentions)
Rna extraction kit, by Takara Bio (1 mentions)
Erk1 2, by Abcam (1 mentions)
Mmp 9, by Abcam (1 mentions)
Fluorescence imaging system, by Bio-Rad (1 mentions)
Puerarin, by Merck Group (1 mentions)
Mtt kit, by Promega (1 mentions)
Phosphorylated erk1 2 p erk1 2, by Abcam (1 mentions)
Transwell chamber, by BD (1 mentions)
4 protocols using microscopic imaging system
1
MTT Assay for Cell Viability
2
Breast Cancer Cell Invasion and Migration
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Cell invasion and migration assays were determined by using Transwell chambers (6.5 mm insert, 24-well plate, 8.0 μm polycarbonate membrane, BD Bioscience, Sparks, MD, USA) with or without Matrigel. Briefly, 4 × 104 or 2 × 104 cells/200 μL of breast cancer cell suspension was prepared separately in serum-free DMEM medium and plated in the upper chamber. Complete medium containing 10% FBS DMEM was added to the lower chamber. After culturing for 24 h, the upper chamber cells were wiped off using cotton swabs, and the chamber was fixed with 75% ethanol and stained with crystal violet. The cells were observed with a Leica microscopic imaging system. Ten fields were randomly selected for photography and statistical analysis.
A wound healing assay was also used to assess the migration of breast cancer cells. Breast cancer cells were seeded in 6-well plates and cultured until confluent. A straight line was scratched using a 200 μL pipettor spear head in each well. The 6-well plate was washed twice with PBS buffer to remove floating cells. The cells were cultured with serum-free DMEM medium. At 24, 48, and 72 h, scratch wound healing was recorded using an inverted microscope, and the scratch width was measured by Image J software for statistical analysis.
A wound healing assay was also used to assess the migration of breast cancer cells. Breast cancer cells were seeded in 6-well plates and cultured until confluent. A straight line was scratched using a 200 μL pipettor spear head in each well. The 6-well plate was washed twice with PBS buffer to remove floating cells. The cells were cultured with serum-free DMEM medium. At 24, 48, and 72 h, scratch wound healing was recorded using an inverted microscope, and the scratch width was measured by Image J software for statistical analysis.
3
Histological Examination of Brain Tissues
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Brain tissues of treated and control rats were fixed in 10% buffered formalin solution in labeled bottles. Tissues were stained with hematoxylin-eosin (H-E) and examined under a microscopic imaging system (Leica Microsystems GmbH, Wetzlar, Germany).
4
Histological Assessment of Bone Samples
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Bone samples were fixed for 48 hrs in 10 % buffered formalin solution, decalcified for 20 days using Cal-X II (Thermo-Fisher Scientific), processed with serial dilutions of ethanol, cleared in xylene and implanted in Paraplast tissue medium. Sections of 5 µm thick were cut, and stained with hematoxylin and eosin (H&E) for histological examination of tissue samples or Masson's Goldner Trichrome staining (Sigma-Aldrich) for the differentiation of mineralized and nonmineralized areas in the bone matrix [37] . Six non-overlapping fields were arbitrarily picked and investigated from every H & E stained bone section for the determination of mean trabecular bone area, mean trabecular bone width, and mean cortical bone width. Fields selected from Masson's Goldner trichrome-stained sections were utilized to determine the relative area percentage of the non-mineralized bone matrix known as osteoid. Histopathological examination was performed using microscopic imaging system (Leica Microsystems GmbH, Germany).
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