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Lipozyme 435

Manufactured by Novozymes
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Sourced in Denmark

Lipozyme 435 is a lipase enzyme immobilized on a macroporous acrylic resin. It is used as a biocatalyst for a variety of lipid-modifying reactions. The enzyme has high activity and operational stability, making it suitable for industrial applications.

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Lab products found in correlation

Ethyl acetate, by Tianjin Damao (3 mentions) Phosphoric acid, by Tianjin Damao (3 mentions) Ethanol, by Tianjin Damao (3 mentions) Glycerol, by Tianjin Damao (3 mentions) Vinyl laurate, by Tokyo Chemical Industry (1 mentions) Novozym 435, by Novozymes (1 mentions) Acetonitrile for hplc, by Merck Group (1 mentions) Lipozyme rm, by Novozymes (1 mentions) 5972 series, by Hewlett-Packard (1 mentions) Pack fa, by YMC (1 mentions) Avance 400, by Bruker (1 mentions) 5890 series 2, by Hewlett-Packard (1 mentions)

5 protocols using lipozyme 435

1

Lipase Enzyme Screening for Synthesis

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Vinyl laurate was purchased from Tokyo Chemical Industry Co., Ltd. (TCI Europe, Zwijndrecht, Belgium). Lipase formulations: Novozym 435®, Lipozyme 435® and Novozym NS 81356 were given by Novozymes (Bagsværd, Denmark). CalB Immo Plus® was given by c-Lecta (Leipzig, Germany). CalA Immo 150, Lipase TL CLEA, Lipase CA CLEA and all other chemicals were purchased from either Carl Roth GbmH & Co. KG (Karlsruhe, Germany) or Sigma Aldrich Chemie GmbH (Taufkirchen, Germany) if not stated otherwise. The other 9 lipase formulations were acquired in the Novozymes Lipase Screening Kit purchased from Strem Chemicals (Newburyport, MA, USA).
Delavault A., Opochenska O., Laneque L., Soergel H., Muhle-Goll C., Ochsenreither K, & Syldatk C. (2021). Lipase-Catalyzed Production of Sorbitol Laurate in a “2-in-1” Deep Eutectic System: Factors Affecting the Synthesis and Scalability. Molecules, 26(9), 2759.
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2

Enzymatic Synthesis of Lipid-Based Products

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Glycerol (purity ≥ 99.0%), ethanol (purity ≥ 99.7%), ethyl acetate (purity ≥ 99.5%) and phosphoric acid (purity ≥ 85.0%) were purchased from Tianjin Damao Chemical Reagent Co. (China). Lauric acid (purity ≥ 98.0%), SB (purity ≥ 99.0%), PS (purity ≥ 99.0%), and sodium hydroxide (purity ≥ 98.0%) were obtained from Aladdin Biochemical Technology Company (China). Lipozyme 435 was obtained from Novozymes Co. (Denmark). All water is purified by Mili-Q (Advantage A10, Merck KgaA, Germany).
Zhang S., Xiong J., Lou W., Ning Z., Zhang D, & Yang J. (2020). Antifungal Effect of Triglycerol Monolaurate Synthesized by Lipozyme 435-Mediated Esterification. Journal of Microbiology and Biotechnology, 30(4), 561-570.
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3

Lipase-catalyzed Stereoselective Reactions

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In this study, we have employed different commercial immobilized lipase. Lipozyme®TL (TLL-IM), Lipozyme®435 (L435), Lipozyme®RM (RML-IM) and LipuraSelect (LS-IM) were kindly donated by Novozymes Spain (Madrid, Spain). Triacetin, (R)- and (S)-methyl mandelate, zinc chloride (ZnCl2), copper chloride (CuCl2), cobalt chloride (CoCl2), sodium chloride (NaCl) and acetonitrile for HPLC (gradient grade, ≥99.9%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All other reagents were of analytical grade.
Guimarães J.R., Carballares D., Tardioli P.W., Rocha-Martin J, & Fernandez-Lafuente R. (2022). Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts. Molecules, 27(14), 4486.
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4

Efficient Synthesis and Characterization of Vanillin Derivatives

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General. In the following examples, the structures of the synthesized compounds were identified by nuclear magnetic resonance spectroscopy (Bruker AVANCE400 (400 MHz)). GC-MS were measured using 5890SERIESII, 5972SERIES, and 7673CON-TROLLER, all from Hewlett Packard (USA). Vanillin (7) was purchased from Rhodia (France) (now Solvay Aroma Performance, Belgium). Immobilized food-grade lipase preparation of Novozym 435 FG (renamed Lipozyme 435) was purchased from Novozymes (Denmark). All other reagents were obtained from commercial sources and were of the highest purity. The content of MNA (12) in DCT (2) was calculated from the peak Notes: Reactions were carried out at 47 °C in a 50 mol scale under reduced pressure (9.2 kPa) (Table 2, Entry 7).
integral value of the nuclear magnetic resonance spectrum or analyzed using a YMC fatty acid analysis kit (YMC-Pack FA, YMC Co. Ltd, Japan).
Analytical conditions 1. HPLC measurement conditions for VOH (8)
Kurosawa W., Nakano T, & Amino Y. (2017). Practical large-scale production of dihydrocapsiate, a nonpungent capsaicinoid-like substance. Bioscience, biotechnology, and biochemistry, 81(2).
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5

Enzymatic Transesterification of Soybean Oil

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Soybean oil was purchased from Wilmar Cereal and Oil Co. Ltd. (Guangzhou, China) . Lipozyme 435 (immobilized Candida Antarctica) was obtained from Novozymes A/S (Bagsvaerd, Denmark) . Glycerol was acquired from Tianjin Chemical Reagent Factory (Tianjin, China) . All solvents and chemicals used were of analytical grade.
Zhang N., Yang X., Fu J., Chen Q., Song Z, & Wang Y. (2016). Production of Diacylglycerol-enriched Oil by Glycerolysis of Soybean Oil using a Bubble Column Reactor in a Solvent-free System. Journal of oleo science, 65(3).
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