The levels of the inflammatory factors IL-6, TNF-α, iNOS and COX2 in articular cartilage tissues of normal and OA mice were analysed using the corresponding kits,including the mouse IL-6 ELISA kit (BMS603-2; Invitrogen), mouse TNF-α ELISA kit (CSB-E04744m; Cusabio), mouse iNOS ELISA kit (ab253219; Abcam), and mouse COX2 ELISA kit (CSB-E12910m; Cusabio), according to the manufacturer’s instructions.
Mouse inos elisa kit
Manufactured by Abcam
Sourced in United States
The Mouse iNOS ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the levels of inducible nitric oxide synthase (iNOS) in mouse samples. The kit utilizes a specific antibody coated on a microplate to capture iNOS, and a detection antibody conjugated to an enzyme for colorimetric quantification.
Automatically generated - may contain errors
Lab products found in correlation
Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions)
Mouse il 6 elisa kit, by Thermo Fisher Scientific (1 mentions)
Mouse tnf α elisa kit, by Cusabio (1 mentions)
Enhanced chemiluminescence detection kit, by Bio-Rad (1 mentions)
Api zym kit, by bioMérieux (1 mentions)
Il 6 elisa kit, by Thermo Fisher Scientific (1 mentions)
Mouse tnf α, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit, by Thermo Fisher Scientific (1 mentions)
Dc protein assay kit, by Bio-Rad (1 mentions)
Pierce rapid gold bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Ifn γ, by R&D Systems (1 mentions)
5 protocols using mouse inos elisa kit
1
Inflammatory Factors in Osteoarthritis
2
Microbial Culture and Analysis Techniques
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lactobacilli MRS broth for bacterial cultures was acquired from BD Biosciences (Franklin Lakes, NJ, USA). For cell culture, phosphate-buffered saline (PBS), fetal bovine serum (FBS), penicillin/streptomycin (P/S), DMEM, and RPMI 1640 were acquired from HyClone (Logan, UT, USA). The API ZYM® kit was obtained from BioMérieux (Marcy-l’Étoile, France). RevertAid First Strand cDNA Synthesis Kit and RNeasy® Mini Kit were obtained from Thermo Fisher Scientific (Waltham, MA, USA) and QIAGEN (Hilden, Germany), respectively. SYBR Green PCR Master Mix for the semi-quantitative real-time RT-PCR was acquired from PikoReal 96 (Scientific Pierce, Waltham, MA, USA). The mouse iNOS ELISA kit was purchased from Abcam (Cambridge, USA). Mouse TNF-α, Mouse IL-1beta, and IL-6 ELISA kit were used from Invitrogen (Carlsbad, CA, USA). The DC protein assay kit and enhanced chemiluminescence detection kit were used from Bio-Rad Laboratories, Inc. (Hercules, CA, USA), and the antibodies were used from Santa Cruz Biotechnology (Dallas, TX, USA). Other materials were used from Sigma-Aldrich (St. Louis, MO, USA).
3
Quantifying Neuroinflammatory Biomarkers in Brain Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GABA, β-secretase, Aβ42, and iNOS levels were measured by enzyme-linked immunosorbent assay (ELISA) as follows: GABA by mouse gamma-aminobutyric acid ELISA kit, MyBioSource, USA, β-secretase by mouse β-secretase ELISA kit, MyBioSource, USA, Aβ42 by mouse amyloid beta peptide 1–42 ELISA kit, MyBioSource, USA, and iNOS by mouse iNOS ELISA kit, Abcam, U.K.
Briefly, brain tissue homogenate samples were added to the appropriate pre-coated wells and incubated at 37 °C for 1 h. After washing with a washing buffer, the labeled specific antibody solution was pipetted and incubated at 37 °C for another hour. Substrate solutions A and B were added into the wells and incubated at 37 °C for 10 min to develop a colored product. The stop solution was finally added, and the optical density of the developed color was measured at 450 nm by a microplate reader (SunriseTM, TECAN, Switzerland).
Briefly, brain tissue homogenate samples were added to the appropriate pre-coated wells and incubated at 37 °C for 1 h. After washing with a washing buffer, the labeled specific antibody solution was pipetted and incubated at 37 °C for another hour. Substrate solutions A and B were added into the wells and incubated at 37 °C for 10 min to develop a colored product. The stop solution was finally added, and the optical density of the developed color was measured at 450 nm by a microplate reader (SunriseTM, TECAN, Switzerland).
4
Quantifying iNOS in Heart Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of inducible nitric oxide synthase in heart tissue or cell homogenates was evaluated using the Mouse iNOS ELISA kit (ab253219; Abcam) or NOS2/iNOS (Rat) Elisa Kit (E4649; BioVision) according to the manufacturer's instructions.
5
Measuring Microglial iNOS Expression
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!