iRoot BP Plus and MTA (Dentsply Tulsa Dental, Tulas, OK, USA) were prepared according to the manufacturer’s instructions and placed into sterile cylinder plastic moulds (13 mm in diameter and 1 mm in height). The materials were stored at 37℃ and 100% humidity until completely solidification. After separation from the moulds, samples were incubated in 1 mL of α-MEM, (HyClone, UT, USA) for 72 h at 37℃ to produce the material extract, followed by filtration and preparation of several dilutions (undiluted, 1/2 and 1/4) of the conditioned medium supplemented with 15% FBS for use. The pH of the extracts was measured with a pH meter (INESA Scientific Instrument Co., Shanghai, China). The ratio of material surface area to medium volume was set at approximately 3 cm2 mL-1 in accordance with the guidelines of the International Organisation for Standardisation 10993 (12: 10.3.3).
Ph meter
Manufactured by INESA
Sourced in China
The PH meter is a device used to measure the pH, or acidity/alkalinity, of a solution. It consists of a pH electrode and a meter that displays the pH reading. The device is designed to provide accurate and reliable pH measurements.
Automatically generated - may contain errors
Lab products found in correlation
Evolution 201 220, by Thermo Fisher Scientific (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Multi n c 3100 analyzer, by Analytik Jena (1 mentions)
Agilent 7900, by Agilent Technologies (1 mentions)
Agilent 6890n, by Agilent Technologies (1 mentions)
Gas chromatography, by Agilent Technologies (1 mentions)
Masslynx 4, by Waters Corporation (1 mentions)
Xevo g2 xs qt, by Waters Corporation (1 mentions)
Zeta potential analyzer, by Malvern Panalytical (1 mentions)
Seal autoanalyzer 3 hr, by SEAL Analytical (1 mentions)
Electrical conductivity meter, by INESA (1 mentions)
Toc l, by Shimadzu (1 mentions)
Z 500, by Hitachi (1 mentions)
Pal 1 handheld refractometer, by Atago (1 mentions)
Pocket refractometer pal 1, by Atago (1 mentions)
18 protocols using ph meter
1
Cytotoxicity Evaluation of Dental Materials
2
Quantification of Oxalic Acid in Sclerotinia
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Five agar plugs (6 mm) from the advancing edge of each S. sclerotiorum strain were transferred into 5 ml PDA medium with 50 mg/L bromophenol blue and incubated at 20°C with shaking at 150 rpm for 2 days. Prior to assaying the concentration of OA in the solution, a standard curve was generated using OA standard samples with a spectrometer (Evolution™ 201/220, ThermoFisher, USA). Meanwhile, the pH value of the solution was measured with a pH meter (INESA, China), and the expression of SsOAH was assayed in S. sclerotiorum strains by qRT-PCR.
Research Funds for the Central Universities (XDJK2018AA004 and XDJK2018B022). We sincerely acknowledge Dr. Pradeep Kachroo and Dr. Zhonglin Mou for critical comments.
Wei L, Jian H, Lu K, Filardo F, Yin N, Liu L, Qu C, Li W, Du H, Li, J (2016) Genome-wide association analysis and differential expression analysis of resistance to Sclerotinia stem rot in Brassica napus. (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
Research Funds for the Central Universities (XDJK2018AA004 and XDJK2018B022). We sincerely acknowledge Dr. Pradeep Kachroo and Dr. Zhonglin Mou for critical comments.
Wei L, Jian H, Lu K, Filardo F, Yin N, Liu L, Qu C, Li W, Du H, Li, J (2016) Genome-wide association analysis and differential expression analysis of resistance to Sclerotinia stem rot in Brassica napus. (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
3
Detailed Protocols for Novel Coronavirus Antibody Assay
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All buffers and solutions were prepared using ultrapure water purified by a Milli‐Q purification system (Millipore). MES cold buffer was prepared using a pH meter (Shanghai INESA Scientific Instrument Co., Ltd.,). The latex microspheres were centrifuged in a frozen centrifuge (Shanghai Anting Scientific Instrument Factory,) and were incubated in a rotary culture apparatus (Haimen Kylin‐Bell Lab Instruments Co., Ltd.,). The centrifuged latex microspheres were subjected to ultrasound in a JY99‐IIDN ultrasonic cell crusher (Ningbo Xinzhi Biotechnology Co., Ltd.,). The latex microsphere pads and antibody‐coated were dried in an air‐blast drying oven (Shanghai Jing Hong Laboratory Instrument Co., Ltd.,). The recombinant mouse anti‐human novel coronavirus secondary antibody and goat anti‐mouse IgG were coated using the BioDot‐XYZ3210 three‐dimensional spraying platform (Shanghai Kinbio Tech Co., Ltd.,). The test strips were cut using a BioDot‐CM4000 cutting machine (Shanghai Kinbio Tech Co., Ltd.,).
4
Soil Heavy Metal Concentrations in Qinghai-Tibet
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The study area locates in the northeastern Qinghai-Tibet Plateau. Field sampling was performed during May 31th to June 13th, 2016. Total 70 topsoil (0e20 cm) samples were collected (Fig. S1), covering the main industrial, mining, and agricultural zones and main traffic lines of the study area. The samples were in situ homogenized and stored in the sample bags until back to the laboratory. The soil samples were air dried at the room temperature, and then passed through 0.074 mm sieve for chemical analysis.
Soil pH was determined with the supernatants of water-soil ratio of 2.5:1 using a pH meter (Shanghai INESA Scientific Instrument Co., China) . Soil total organic carbon (TOC) was measured by a multi N/C 3100 analyzer (Analytik Jena AG, Germany). Microwavedigested soil samples were analyzed by an Agilent7900 inductively coupled plasma mass spectrometry (ICP-MS, Agilent Inc, USA). Concentrations of 12 typical heavy metals including vanadium (V), chromium (Cr), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), molybdenum (Mo), cadmium (Cd), tin (Sn), antimony (Sb), mercury (Hg), and lead (Pb) were determined.
Soil pH was determined with the supernatants of water-soil ratio of 2.5:1 using a pH meter (Shanghai INESA Scientific Instrument Co., China) . Soil total organic carbon (TOC) was measured by a multi N/C 3100 analyzer (Analytik Jena AG, Germany). Microwavedigested soil samples were analyzed by an Agilent7900 inductively coupled plasma mass spectrometry (ICP-MS, Agilent Inc, USA). Concentrations of 12 typical heavy metals including vanadium (V), chromium (Cr), cobalt (Co), nickel (Ni), copper (Cu), zinc (Zn), molybdenum (Mo), cadmium (Cd), tin (Sn), antimony (Sb), mercury (Hg), and lead (Pb) were determined.
5
SCFA Analysis of Digestive Samples
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The pH value was measured by a pH meter (INESA Scientific Instrument Co., Ltd., Shanghai, China).
Digestive samples were centrifuged at 7104× g for 15 min at room temperature and the supernatants collected for SCFA analysis. The SCFAs were assayed by the method of Di et al. [21 (link)] using an Agilent 6890 N gas chromatography system (Agilent, Santa Clara, CA, USA) equipped with a DP-FFAP column (0.32 mm × 0.25 µm × 30 m) and a flame ionization detector. Nitrogen was set as the carrier gas, with a total flow of 19 mL/min. A 1.0 µL sample was injected into the GC. The experimental conditions were programmed: oven temperature was initially set at 60 °C for 1 min, increased to 120 °C at a rate of 6 °C/min, increased to 220 °C at a rate of 20 °C/min, and then held at 220 °C for 9 min. The temperatures of the injector and detector were identically set to 250 °C.
Digestive samples were centrifuged at 7104× g for 15 min at room temperature and the supernatants collected for SCFA analysis. The SCFAs were assayed by the method of Di et al. [21 (link)] using an Agilent 6890 N gas chromatography system (Agilent, Santa Clara, CA, USA) equipped with a DP-FFAP column (0.32 mm × 0.25 µm × 30 m) and a flame ionization detector. Nitrogen was set as the carrier gas, with a total flow of 19 mL/min. A 1.0 µL sample was injected into the GC. The experimental conditions were programmed: oven temperature was initially set at 60 °C for 1 min, increased to 120 °C at a rate of 6 °C/min, increased to 220 °C at a rate of 20 °C/min, and then held at 220 °C for 9 min. The temperatures of the injector and detector were identically set to 250 °C.
6
Electrochemical Analysis of Modified Fly Ash
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SWASV was performed with a VersaSTAT3 electrochemical station (Princeton, Advanced Measurement Technology, Inc., USA). A conventional three-electrode system consisted of a carbon paste working electrode with 3.0 mm diameter, a counter electrode made of platinum wire and an Ag|AgCl reference electrode. A 20.0 mL cell was used for the electrochemical measurements. All potentials were given with respect to the Ag|AgCl reference electrode. The pH meter (Shanghai INESA Scientific Instrument Co., Ltd, China) was used to measure pH in this experiment. An ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., Jiangsu, China) was used for the ultrasonication of fly ash. The chemical composition of fly ash used in this experiment was measured with an XRF (PANalytical B.V., Netherlands). The surface morphology and pore structure of fly ash before and after modification were measured with the scanning electron microscope (SEM) and automatic surface area and porosity analyzer, respectively.
7
Soil Chemical and Biological Analysis
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Soil pH was measured with a pH meter (INESA, Shanghai, China), with a water-to-soil ratio of 2.5:1. Soil water content and porosity followed the method of Nong et al. [39 (link)]. Soil organic matter content (SOM) was measured by oxidation–dilution heat method with potassium dichromate according to Lu et al. [40 (link)]. The total and available content of nitrogen, phosphorus and potassium in the soil were measured by the method of Bao [41 ]: total nitrogen (TN) was determined by sulfuric acid digestion–semi-micro Kjeldahl method; available nitrogen (AN) was measured by diffusion dish method; total phosphorus (TP) was measured by molybdenum antimony colorimetry; available phosphorus was measured by carbonate buffer extraction and molybdenum antimony colorimetry; total potassium (TK) was measured by H2SO4–H2O2 digestion flame photometry; available potassium (AK) was measured by NH4OAc extraction flame photometry. Soil enzyme activity was measured using the methods of Ma et al. [42 (link)]: sucrose (SC) activity was determined by 3,5-dinitrosalicylic acid colorimetry; urease (UE) activity was measured by phenol–sodium hypochlorite colorimetry.
8
Yak Blood and Tissue Sampling
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Jugular blood samples (10 mL) of all yaks were collected on day 120 before the morning feeding. The serums were separated after centrifuge at 3500 rpm at 4 °C for 15 min and stored at −20 °C for analysis of serum biochemical and hormonal parameters. Then, six yaks in each group, which were close to the group average weight, were slaughtered humanely by captive bolt stunning and exsanguination according to the National Standard Operating Procedures of Cattle Slaughtering (GB/T 19477-2004). Digesta collected from dorsal, ventral, and caudal areas of the rumen were mixed and filtered through four-layer nylon cloth. Rumen fluid was collected in three 10-mL centrifuge tubes; one centrifuge tube of rumen fluid was immediately used to detect pH value using a pH meter (INESA, Shanghai, China), and the others were stored immediately at −80 °C for ruminal fermentation parameters and bacterial community composition analysis. The longissimus dorsi muscle samples were collected between the 12th and 13th ribs of the left side of carcass, and the intramuscular fatty acid profile was detected by using gas chromatography (Agilent Technologies, Santa Clara, CA, USA) as described in our previous study [12 (link)].
9
HPLC and UPLC-QTOF/MS Analysis of DCF
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The concentration of DCF was determined by a high performance liquid chromatograph (HPLC, Waters 2695, USA) equipped with a symmetry C18 column (5 μm, 4.6 × 150 mm) and a UV detector (Waters 2966, USA) at 276 nm. The mobile phase consisted of methanol and 1‰ acetic acid water solution (75/25, v/v) with a flow rate of 1.0 mL min−1. The column temperature was 30 °C and the sample injection volume was 20 μL. pH was measured by a pH meter (INESA Scientific Instrument Co., Ltd, China). The transformation products of DCF were detected using an ultra performance liquid chromatograph coupled with a quadrupole-time of flight-mass spectrometry (UPLC-QTOF/MS, Waters Xevo G2-XS QT, USA). The chromatographic separation was performed on a C18 column (1.7 μm, 2.1 × 100 mm) with the sample injection volume of 10 μL. The mobile phase contained A (0.1% formic acid in water) and B (acetonitrile) at the flow rate of 0.3 mL min−1. The gradient was 10% B in the initial 0.5 min, linearly increasing to 100% B for 6.5 min, and decreasing back to 10% B for 3 min. The mass spectrum (m/z 50–800) was analyzed in a positive ion mode by electrospray ionization (ESI) with the drying gas temperature of 400 °C and capillary voltage of 2.5 kV. Data were analyzed through Masslynx 4.1 software (Waters, USA).
10
Characterization of Natural Mineral Adsorbents
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The analysis of crystal structures of HNTs and ChNTs was completed by X-ray diffractometer (XRD, Bruker Scientific Instruments Hong Kong CO., Ltd.), and the working conditions are as follows: using 40 kV and 100 mA Cu-Kα target as a radiation source, scanning speed 5°/min, scanning range 3° ≤ 2θ ≤ 70°. The micro-morphologies of HNTs and ChNTs were obtained by scanning electron microscope (SEM, JEOL Ltd.). The relevant data of specific surface area, pore size distribution and other adsorption properties of two kinds of natural minerals were obtained by BET specific surface area test method using automatic specific surface area and pore size distribution analyzer (BET, Quantachrome Instruments Co., Ltd.) and nitrogen as adsorbent. The Zeta potentials of HNTs and ChNTs were measured by Zeta potential analyzer (Malvern Instruments Co., Ltd.). The zero electric points of two kinds of minerals were determined by pH meter (Shanghai INESA Scientific Instrument Co., Ltd.). The change of the structure and composition of the adsorbent under different pollutant concentrations was characterized by Fourier transform infrared spectroscopy (FTIR).
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