Invivomab anti mouse ifnγ

I) For CD4⁺ T cell depletion, intraperitoneal injection of Ultra-LEAF™ Purified anti-mouse CD4 Antibody (10 μg/g, clone GK1.5, BioLegend, San Diego, CA, USA) or corresponding isotype antibody (Ultra-LEAF Purified Rat IgG2b, κ Isotype Ctrl Antibody, clone RTK4530, 10 μg/g, BioLegend) was performed in mice on day 15, 25, and 40 PMI. ii) For CXCR3 blockade, intraperitoneal injection of Ultra-LEAF™ Purified anti-mouse CD183 (CXCR3) Antibody (10 μg/g, clone CXCR3-173, BioLegend) or corresponding isotype antibody (Armenian Hamster IgG, clone: HTK888, BioLegend) was performed in mice every 7 days since day 15 PMI. iii) For systemic IFN-g blocking, mice were injected i.p. with 200 mg of anti-IFN-g antibody (InVivoMAb anti-mouse IFNγ, BioXcell, clone XMG1.2) every 3 days since day 15 PMI. An IgG1 isotype antibody (HRPN, BioXcell) was used to serve as control. iv) To block VCAM-1, a InVivoMAb anti-mouse VCAM-1 (10 mg/kg, BioXcell; corresponding isotype antibody: InVivoMAb rat IgG1 isotype control, clone HRPN, BioXcell) was administrated intravenously in MB-injected mice every 3 days since day 15 PMI. For retinal evaluation in abovementioned in vivo antibody intervention experiments, one retina from each recipient mouse was randomly selected and used for RGC density, while the other was for Iba1 and GFAP staining.