Ki67 pe b56

Manufactured by BD

Ki67-PE (B56) is a fluorescently-labeled antibody that binds to the Ki67 protein, a marker of cellular proliferation. This product is intended for use in flow cytometry and other immunodetection applications to identify and quantify proliferating cells.

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Lab products found in correlation

Cd38 fitc, by STEMCELL (1 mentions) Cytofix cytoperm, by BD (1 mentions) Alexa fluor 700, by BD (1 mentions) Permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Foxp3 apc, by Thermo Fisher Scientific (1 mentions) Cd4 fitc rpa t4, by BD (1 mentions) Fixation permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Facscalibur, by BD (1 mentions) Cd3 apc cy7 sp34 2, by BD (1 mentions) Bv421 conjugated streptavidin, by BioLegend (1 mentions) Live dead fixable blue viability dye, by Thermo Fisher Scientific (1 mentions) Transcription factor buffer set, by BD (1 mentions)

Close spelling variants of this product

Ki67-PE (clone B56; Anti-Ki67-PE (B56; Anti-human Ki67-PE (clone B56) Ki67 (B56) PE Ki67

4 protocols using ki67 pe b56

Plasmablast Frequency in SIV Vaccinated Macaques

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We measured the frequency of plasmablasts in the peripheral blood of twenty macaques vaccinated with ALVAC-SIV/gp120 and twenty macaques vaccinated with NYVAC-SIV/gp120 before vaccination and at week 25 (7 days after the last immunization). To stain the cells, the following markers were labeled: CD3 (SP34^-2), CD14 (M5E2), CD16 (3G8), and CD56 (B159), all in ALEXAFluor700 (BD Biosciences); CD19^- PE^-Cy5 (J3-119, Beckman Coulter), CD20^- Qdot650 (2H7, eBiosciences), CD38^- FITC (Clone AT-1, StemCell), CD39^- BV421 (MOCP-21, BioLegend), Ki67^- PE (B56, BD Biosciences), and CD183^-PE^-CF594 (CXCR3; 1C6 BD, Biosciences). Dr. A. A. Ansari kindly provided the anti-α4β7 (Act-1) reagent (cat. #11718) through the NIH AIDS (NIAD) Reagent Program, Division of AIDS. Cytofix/Cytoperm (BD Biosciences) was used to allow intracellular staining. LSR II (BD Biosciences) was used to evaluate acquisition and the resulting data were analyzed with FlowJo (TreeStar). Gating of lineage negative (CD3^- CD14^- CD16^- CD56^-) CD20⁺ CD21^- Ki67⁺ CD38⁺ CD39⁺ was used to identify plasmablasts [78 (link)]. The expression of CXCR3 or α4β7 on the plasmablasts was used to calculate the frequency of the CXCR3⁺ and α4β7⁺ plasmablasts.

Gorini G., Fourati S., Vaccari M., Rahman M.A., Gordon S.N., Brown D.R., Law L., Chang J., Green R., Barrenäs F., Liyanage N.P., Doster M.N., Schifanella L., Bissa M., Silva de Castro I., Washington-Parks R., Galli V., Fuller D.H., Santra S., Agy M., Pal R., Palermo R.E., Tomaras G.D., Shen X., LaBranche C.C., Montefiori D.C., Venzon D.J., Trinh H.V., Rao M., Gale M J.r., Sekaly R.P, & Franchini G. (2020). Engagement of monocytes, NK cells, and CD4+ Th1 cells by ALVAC-SIV vaccination results in a decreased risk of SIVmac251 vaginal acquisition. PLoS Pathogens, 16(3), e1008377.

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Phenotyping T Regulatory Cells

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PBMC surface antigens were stained with CD4-FITC (RPA-T4), CD25-APC (MA251), Ki67-PE (B56), and CD127-PE (hIL-7R-M21) (BD Bioscience, San Diego, CA). After washing to remove unbound antibody, cells were incubated on ice for 30 minutes in fixation/permeabilization buffer (eBioscience, San Diego, CA). Cells were washed again with 1X permeabilization buffer (eBioscience), pelleted, and stained with FOXP3-APC (PCH101, eBioscience). Samples were acquired on BD FACS Calibur (BD, San Diego, CA) and analyzed with FlowJo (TreeStar, Ashland, OR). T_Regs were defined as CD25⁺FOXP3⁺ of CD4⁺ lymphocytes.

Batich K.A., Reap E.A., Archer G.E., Sanchez-Perez L., Nair S.K., Schmittling R.J., Norberg P., Xie W., Herndon JE I.I., Healy P., McLendon R.E., Friedman A.H., Friedman H.S., Bigner D., Vlahovic G., Mitchell D.A, & Sampson J.H. (2017). Long-term Survival in Glioblastoma with Cytomegalovirus pp65-targeted Vaccination. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(8), 1898-1909.

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Cell Proliferation Monitoring by Flow Cytometry

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To monitor cell proliferation, cells were stained for surface markers followed by fixation and permeabilization with in specific buffers (eBioscience), and stained with Ki67 (PE, B56, BD Biosciences) and analyzed by flow cytometry.

Thome J.J., Yudanin N., Ohmura Y., Kubota M., Grinshpun B., Sathaliyawala T., Kato T., Lerner H., Shen Y, & Farber D.L. (2014). Spatial map of human T cell compartmentalization and maintenance over decades of life. Cell, 159(4), 814-828.

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Analyzing Env-specific GC B Cell Responses

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To assess Env-specific GC B cell responses, frozen LN cell suspensions were thawed and washed in R10, then stained with live/dead fixable blue viability dye (Invitrogen), tetramer Env probes in AF488 and BV421 for 30 min at 4°C. Cells were subsequently stained with anti-human CD20 BV570 (2H7, Biolegend), and CD3 APC-Cy7 (SP34–2, BD Biosciences) for an additional 20 min at 4°C. Cells were permeabilized using the transcription factor buffer set (BD Biosciences) and stained intracellularly for anti-human IgG BV786 (G18–145, BD Biosciences), BCL6 PE-Cy7 (K112–91, BD Biosciences), and Ki67 PE (B56, BD Biosciences). Tetramer Env probes were prepared by incubation of 4-fold molar excess of avi-tag biotinylated 1086 Env protein with either streptavidin-conjugated AF488 (Invitrogen) or streptavidin-conjugated BV421 (Biolegend).

Ols S., Yang L., Thompson E.A., Pushparaj P., Tran K., Liang F., Lin A., Eriksson B., Karlsson Hedestam G.B., Wyatt R.T, & Loré K. (2020). Route of Vaccine Administration Alters Antigen Trafficking but Not Innate or Adaptive Immunity. Cell reports, 30(12), 3964-3971.e7.

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