For SDS-PAGE, 1–20 μg of total protein samples was denatured with Laemmli loading buffer (Bio-Rad). The samples were run on a pre-casted NuPAGE 4–12% Bis–Tris gel (Invitrogen). After electrophoresis, the proteins were transferred to nitrocellulose (Whatman), which was incubated overnight with the primary antibody at 4°C. The following antibodies were used: mouse αEGFP (Millipore; 1:5000), rabbit αGAPDH (Santa Cruz Biotechnology; 1:1000), rabbit αSRSF7 (Santa Cruz Biotechnology; 1:1000), mouse αSRSF3 (7B4, REF), goat αNXF1 (Santa Cruz Biotechnology; 1:750), rabbit αHistone H3 (Abcam; 1:10 000), rabbit αRNA Pol II (Santa Cruz Biotechnology; 1:2000), donkey α-rabbit-HRP (GE-Health care; 1:8000), donkey α-goat-HRP (Sigma; 1:8000), goat α-mouse-HRP (Sigma; 1:10 000).
Donkey α rabbit hrp
Manufactured by GE Healthcare
Donkey α-rabbit-HRP is a secondary antibody conjugate designed for use in immunoassay and immunohistochemistry applications. It is composed of donkey anti-rabbit antibodies labeled with horseradish peroxidase (HRP) enzyme. This conjugate can be used to detect and visualize rabbit primary antibodies in various experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Nitrocellulose, by Cytiva (1 mentions)
Laemmli loading buffer, by Bio-Rad (1 mentions)
Rabbit α gapdh, by Santa Cruz Biotechnology (1 mentions)
Rabbit α histone h3, by Abcam (1 mentions)
Goat α mouse hrp, by Merck Group (1 mentions)
Nupage 4 12 bis tris gel, by Thermo Fisher Scientific (1 mentions)
Streptavidin magnetic sepharose beads, by GE Healthcare (1 mentions)
Sheep α mouse hrp, by GE Healthcare (1 mentions)
2 protocols using donkey α rabbit hrp
1
Western Blot Protein Detection
2
Affinity Purification of Proteins
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The monoclonal mouse-α-GP1 H3C8 antibody was a kind gift from Carolyn Wilson of the Food and Drug Administration. Rat-α-HA-HRP 3F10, rabbit α-GAPDH and mouse-α-beta tubulin were from Sigma Aldrich. The monoclonal rabbit-α-NPC1 (ab134113) was from Abcam. The anti-beta tubulin mouse monoclonal antibody was from Sigma Aldrich. The sheep-α-mouse-HRP, the donkey-α-rabbit-HRP and the streptavidin magnetic sepharose beads were from GE Healthcare. The α-rabbit IR800, α-mouse IR680, and α-rat IR680 antibodies were from Licor. The mouse α-VSV-M antibody (23H2) was a gift from Michael Whitt.
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