Phosphatidylserine externalization was detected using an Annexin V/propidium iodide (PI) staining kit (Life Technologies) following the manufacturer’s instructions. Annexin V/PI positive cells were detected using a Beckman Coulter Gallios 561 Flow Cytometer.
Annexin 5 propidium iodide staining kit
Manufactured by Thermo Fisher Scientific
Sourced in Austria
The Annexin V/propidium iodide (PI) staining kit is a laboratory reagent used for the detection and quantification of apoptotic and necrotic cells. It contains Annexin V, which binds to phosphatidylserine, and propidium iodide, which stains DNA. The kit allows for the identification of different cell populations based on their staining patterns.
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Lab products found in correlation
9 protocols using annexin 5 propidium iodide staining kit
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Annexin V/PI Apoptosis Assay
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Annexin V/PI Staining for Apoptosis
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Annexin-V/PI Apoptosis Assay
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After 72h of treatment of cells with cetuximab and/or celecoxib (6-well plates, 3 × 106 cells/well) the fraction of apoptotic cells was estimated by using an Annexin-V/propidium iodide (PI) staining kit (Bender MedSystems, Viena, Austria), according to manufacturer´s recommendations. Binding of fluorescein-conjugated Annexin-V and PI was measured by flow cytomewtry (FACSCalibur; BD, Franklin Lakes, NJ, USA) to quantify the percentage of apoptotic cells.
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Quantification of Apoptosis by Annexin-V/PI Flow Cytometry
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The fraction of apoptotic cells was estimated after 48h of treatment of cells growing in the absence or in the presence of EGF (100 ng/mL) with different doses of AEE788 and/or celecoxib in 6-well plates at a density of 3 × 106 cells/well. Viability was assessed by using an Annexin-V/propidium iodide (PI) staining kit (Bender MedSystems, Vienna, Austria), according to manufacturer´s recommendations. Binding of fluorescein-conjugated Annexin-V and PI was measured by flow cytometry (FACSCalibur; BD, Franklin Lakes, NJ, USA) to quantify the percentage of apoptotic cells.
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Annexin V/PI Staining for Tumor Cell Death
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Annexin V/Propidium Iodide (PI) Staining Kit (Thermo Fisher Scientific) was used according to the manufacturer’s protocol to characterize tumor cell death and analyzed by using J-Fortessa fluorescence activated cell sorting (Dana-Farber Cancer Institute; Jimmy Fund Flow Cytometry Core, Boston, MA, USA). We used FlowJo software (Treestar, Ashland, OR, USA) to quantify the results.
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Cell Cycle and Apoptosis Analysis
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Cell cycle analysis and apoptotic cell quantification were performed using the Guava cell cycle reagent (Millipore Sigma) or the annexin V/propidium iodide (PI) staining kit (Thermo Fisher Scientific), respectively, according to the manufacturer's instructions. Cells were quantified on a BD™ (BD Biosciences) LSR II flow cytometer and analyzed using FlowJo software (FlowJo, Ashland, OR, USA).
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Cell Cycle and Apoptosis Analysis
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Cell cycle analysis and apoptotic cell quantification were performed using the Guava cell cycle reagent (Millipore Sigma) or the annexin V/propidium iodide (PI) staining kit (Thermo Fisher Scientific), respectively, according to the manufacturer's instructions. Cells were quantified on a BD LSR II flow cytometer and analyzed using FlowJo software (FlowJo).
Cell proliferation was measured using the MTT reagent and cells cultured in 96-well plates. Cells were incubated with MTT for 4 h at 37 °C. After incubation, culture media was removed and 150 μl of DMSO was added in order to solubilize the MTT formazan crystals. Optical density was determined at 590 nm using a TECAN plate reader. At least three independent experiments were performed.
Cell proliferation was measured using the MTT reagent and cells cultured in 96-well plates. Cells were incubated with MTT for 4 h at 37 °C. After incubation, culture media was removed and 150 μl of DMSO was added in order to solubilize the MTT formazan crystals. Optical density was determined at 590 nm using a TECAN plate reader. At least three independent experiments were performed.
8
Cell Cycle and Apoptosis Analysis
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Cell cycle analysis and apoptotic cell quantification was performed using the Guava cell cycle reagent (Millipore Sigma) or the annexin V/propidium iodide (PI) staining kit (Thermo Fisher Scientific), respectively, according to the manufacturer’s instructions. Cells were quantified on a BD LSR II flow cytometer and analyzed using FlowJo software (FlowJo, Ashland, OR, USA).
9
Cell Cycle, Apoptosis, and Proliferation Analysis
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Cell cycle analysis and apoptotic cell quantification was performed using the Guava cell cycle reagent (Millipore Sigma) or the annexin V/propidium iodide (PI) staining kit (Thermo Fisher Scientific), respectively, according to the manufacturer's instructions. Cells were quantified on a BD LSR II flow cytometer and analyzed using FlowJo software (FlowJo, Ashland, OR, USA).
Cell proliferation was measured using the MTT reagent and cells cultured in 96-well plates.
Cells were incubated with MTT for 4 hr at 37°C. After incubated, culture media was removed and 150μl of DMSO was added in order to solubilize the MTT formazan crystals. Optical density was determined at 590 nm using a TECAN plate reader. At least three independent experiments were performed.
Cell proliferation was measured using the MTT reagent and cells cultured in 96-well plates.
Cells were incubated with MTT for 4 hr at 37°C. After incubated, culture media was removed and 150μl of DMSO was added in order to solubilize the MTT formazan crystals. Optical density was determined at 590 nm using a TECAN plate reader. At least three independent experiments were performed.
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