Caulobacter crescentus cells were grown in M2G media lacking xylose to deplete wild-type CcrM (19 (link)). Cells were collected at the exponential phase (OD600 < 0.3) and spotted on agarose pads (1.5%) containing M2G media prior to imaging. Phase-contrast images were obtained using a Leica DMi8 microscope with an HC PL APO 100 × /1.40 oil PH3 objective, Hamamatsu electron-multiplying charge-coupled device (EMCCD) C9100 camera, and Leica Application Suit X software. For computational image analyses, MicrobeJ (20 (link)) was used to determine cell outlines and lengths from phase images.
Application suit x software
Manufactured by Leica
Application Suit X software is a data analysis and visualization tool designed for Leica lab equipment. It provides essential functions for processing and interpreting data generated by Leica instruments. The software enables users to manage, analyze, and present their experimental results effectively.
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Lab products found in correlation
2 protocols using application suit x software
1
Caulobacter crescentus Cell Imaging
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Caulobacter crescentus cells were grown in M2G media lacking xylose to deplete wild-type CcrM (19 (link)). Cells were collected at the exponential phase (OD600 < 0.3) and spotted on agarose pads (1.5%) containing M2G media prior to imaging. Phase-contrast images were obtained using a Leica DMi8 microscope with an HC PL APO 100 × /1.40 oil PH3 objective, Hamamatsu electron-multiplying charge-coupled device (EMCCD) C9100 camera, and Leica Application Suit X software. For computational image analyses, MicrobeJ (20 (link)) was used to determine cell outlines and lengths from phase images.
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Caulobacter crescentus cells were grown in M2G media lacking xylose to deplete wild-type CcrM (19 (link)). Cells were collected at the exponential phase (OD600 < 0.3) and spotted on agarose pads (1.5%) containing M2G media prior to imaging. Phase-contrast images were obtained using a Leica DMi8 microscope with an HC PL APO 100 × /1.40 oil PH3 objective, Hamamatsu electron-multiplying charge-coupled device (EMCCD) C9100 camera, and Leica Application Suit X software. For computational image analyses, MicrobeJ (20 (link)) was used to determine cell outlines and lengths from phase images.
2
Visualizing SARS-CoV-2 Spike Fusion
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HEK293T-ACE2 cells were cotransfected with GFP constructs and parental D614G S, Omicron S, or their related mutants, followed by imaging the syncytium formation under a Leica DMi8 fluorescence microscope after 24 h of transfection. The cell-cell fusion efficiency was analyzed by measuring areas of the fused cells from three representative images using Leica Application Suit X software. Scale bars represent 150 μm.
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