DXMa was purchased from LA COOPER (Melun, France). Hydroxypropyl-γ-cyclodextrin (HPγCD, W8HP, DS = 0.6, and Mw = 1576 Da) was a kind gift from ASHLAND (Schaffhausen, Switzerland) and hydroxypropyl-β-cyclodextrin (HPβCD, KLEPTOSE DS = 0.63 and Mw = 1391 Da) was obtained from ROQUETTE (Lestrem, France). CELLUVISC® (sodium carboxymethylcellulose) and VISMED® (sodium hyaluronate) are marketed gels used for the treatment of dry eye syndrome. DEXAFREE® (DXM sodium phosphate 1% solution eye drops), MAXIDEX® (DXM 0.1% suspension eye drops) and BSS® (Alcon Laboratories, Rueil-Malmaison, France) are human authorized ocular medicines. Normal human primary corneal epithelial cells (ATCC PCS 700-010), medium (ATCC PCS-700-030), growth kit (ATCC PCS-700-040), PBS (ATCC 30-2200), trypsin EDTA (ATCC PCS-999-003 and 005), and antibiotics (gentamicin, streptomycin, and amphotericin BATC PCS-999-002) were obtained from LGC standard - ATCC® (Molsheim, France). Thioglycollate with resazurine medium and Tryptic soy broth were obtained from BIOMERIEUX (Craponne, France). ALAMARBLUE® was purchased from BIO-RAD (Marnes-la-Coquette, France) and DMSO from SIGMA-ALDRICH (Lyon, France). Purified water was prepared by DIRECT-Q®3UV water purifier (MILLIPORE, Molsheim, France). All other solvents and chemicals were of HPLC and analytical grade, respectively.
Amphotericin
Manufactured by American Type Culture Collection
Sourced in France, Israel
Amphotericin is a laboratory-grade antifungal agent used in cell culture applications. It is effective against a broad spectrum of fungal species and is commonly used to prevent fungal contamination in cell culture media and other microbiological procedures.
Automatically generated - may contain errors
Lab products found in correlation
Gentamicin, by American Type Culture Collection (1 mentions)
Tryptic soy broth (tsb), by bioMérieux (1 mentions)
Trypsin edta, by American Type Culture Collection (1 mentions)
Growth kit, by American Type Culture Collection (1 mentions)
Streptomycin, by American Type Culture Collection (1 mentions)
Phosphate buffered saline (pbs), by American Type Culture Collection (1 mentions)
Direct q 3uv water purifier, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Alamarblue, by Bio-Rad (1 mentions)
Maxidex, by Alcon (1 mentions)
Hpβcd, by Roquette (1 mentions)
Hydroxypropyl β cyclodextrin, by Roquette (1 mentions)
Sh sy5y cell, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Amphotericin b, by Sartorius (1 mentions)
2 protocols using amphotericin
1
Detailed Characterization of Ophthalmic Formulations
2
Differentiation of Neuroblastoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human embryonic kidney 293T (HEK293T) ADAM10 knockout cells (Brummer et al., 2018 (link)) (provided by Dr. Michael G. Tomlinson, University of Birmingham) were cultured in Dulbecco's Modified Eagle's Medium (DMEM high glucose, Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% penicillin–streptomycin, 1% amphotericin B, and 1% non-essential amino acids (all from Biological Industries, Israel).
Neuroblastoma SH-SY5Y cells (ATCC, CRL 2266) were cultured in DMEM:F12 (1:1) supplemented with 10% heat-inactivated FBS, 1% penicillin–streptomycin, 1% amphotericin, and 1% non-essential amino acids. For the induction of neuronal differentiation, cells were seeded and allowed to adhere for 24 h. Next, the media was replaced, and cells were cultured in growth media containing 1% heat-inactivated FBS and 10 μM all-trans retinoic acid (Sigma) for 4 days (adapted from Encinas et al., 2002 (link)).
All cultures were maintained in a humidified 37°C incubator with a 5% CO2 atmosphere.
Neuroblastoma SH-SY5Y cells (ATCC, CRL 2266) were cultured in DMEM:F12 (1:1) supplemented with 10% heat-inactivated FBS, 1% penicillin–streptomycin, 1% amphotericin, and 1% non-essential amino acids. For the induction of neuronal differentiation, cells were seeded and allowed to adhere for 24 h. Next, the media was replaced, and cells were cultured in growth media containing 1% heat-inactivated FBS and 10 μM all-trans retinoic acid (Sigma) for 4 days (adapted from Encinas et al., 2002 (link)).
All cultures were maintained in a humidified 37°C incubator with a 5% CO2 atmosphere.
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