Pg5luc reporter
The PG5Luc reporter is a plasmid-based system that contains the firefly luciferase gene under the control of a synthetic promoter. It is designed for the measurement of transcriptional activity in various cell lines.
Lab products found in correlation
5 protocols using pg5luc reporter
Transient Transfection of Mutant PPARγ Plasmids
Optogenetic Regulation of Luciferase Expression
For measurements of Gaussia luciferase activity, 1 µL of cell culture medium was mixed with 100 µL of 2 µM coelenterazine (Sigma, St. Louis, MO, USA) in wells of 96-well reflective bottom plates for bioluminescent assays (Stellar Scientific, Owings Mills, MD, USA). The quantification of bioluminescence was performed using a Victor X-5 plate reader (Perkin Elmer, Waltham, MA, USA). The contrast of 448 nm/567 nm reporter expression was calculated as luciferase activity in samples under illumination with 448 nm light divided by that at 567 nm light.
Quantifying Nuclear Receptor Activity
Transient Transfection and Luciferase Assay for Nuclear Receptor Activity
Transactivation Assay of RXRα
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