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Wnt1 cre mice

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Wnt1-Cre mice are a transgenic mouse line that express the Cre recombinase under the control of the Wnt1 promoter. Wnt1 is a gene involved in embryonic development, particularly in the formation of the midbrain and neural crest cells. The Wnt1-Cre mice allow for the conditional deletion or activation of target genes in cells derived from the midbrain and neural crest during embryonic development.

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Lab products found in correlation

R26 idtr mice, by Jackson ImmunoResearch (2 mentions) R26mtmg mice, by Jackson ImmunoResearch (1 mentions) C57bl 6j mouse, by GemPharmatech (1 mentions) K14 cre mice, by Jackson ImmunoResearch (1 mentions)

13 protocols using wnt1 cre mice

1

Generation of Ikbkap Conditional Knockout Mice

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The generation of Wnt1-Cre; IkbkapLoxP/LoxP (Ikbkap CKO) embryos has been previously described22 (link). Wnt1-Cre mice were purchased from the Jackson Laboratory (stock number 003829) and all strains were maintained on a C57BL/6 J background. For all analyses, Wnt1-Cre; IkbkapLoxP/LoxP E17.5 embryos were used as experimental and Ikbkap+/LoxP littermates were used as controls. All genotyping was performed via routine PCR22 (link). Ikbkap CKO and wild-type alleles were distinguished using the following primers: forward (F), 5′-GCACCTTCACTCCTCAGCAT-3′ and reverse (R), 5′-AGTAGGGCCAGGAGAGAACC-3′. The presence of the Wnt1-Cre allele was detected using the following primers: F, 5′-GCCAATCTATCTGTGACGGC-3′ and R, 5′-CCTCTATCGAACAAGCATGCG-3′. All experiments were performed according to the National Institutes of Health Guide for Care and Use of Laboratory Animals and protocols were approved by the Montana State University Institutional Animal Care and Use Committee.
Goffena J., Lefcort F., Zhang Y., Lehrmann E., Chaverra M., Felig J., Walters J., Buksch R., Becker K.G, & George L. (2018). Elongator and codon bias regulate protein levels in mammalian peripheral neurons. Nature Communications, 9, 889.
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2

Genetically Engineered Mouse Lines

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Brd4 floxed mice were obtained from Keiko Ozato’s laboratory30 , and Wnt1Cre mice were obtained from Jackson Laboratories (#022137). R26mt-mg mice were obtained from Jackson Laboratories68 . Mice were maintained on mixed CD1/B6/129 genetic backgrounds. Littermate embryos were analyzed in all experiments unless otherwise noted. The Institutional Animal Care and Use Committee approved all animal protocols.
Linares-Saldana R., Kim W., Bolar N.A., Zhang H., Koch-Bojalad B.A., Yoon S., Shah P.P., Karnay A., Park D.S., Luppino J.M., Nguyen S.C., Padmanabhan A., Smith C.L., Poleshko A., Wang Q., Li L., Srivastava D., Vahedi G., Eom G.H., Blobel G.A., Joyce E.F, & Jain R. (2021). BRD4 orchestrates genome folding to promote neural crest differentiation. Nature genetics, 53(10), 1480-1492.
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3

Genetic Mouse Models for Neural Crest Studies

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All animal protocols were approved by the Institutional Animal Care and Use Committee at Massachusetts General Hospital (Protocols #2009N000239 and #2013N000115). The following mouse lines were obtained from Jackson Laboratory (Bar Harbor, ME, USA): TauGFP/GFP mice, Ednrb+/− mice, Wnt1::Cre mice, R26-iDTR mice, and PC::G5-tdT mice (Supplementary Table S1). Plp1-GFP mice were kindly gifted by Dr Wendy Macklin, University of Colorado.22 (link) The various breeding schemes and genotypes of controls are summarized in Table 1. Wnt1::Cre mice were crossed with R26-iDTR mice to obtain Wnt1-Cre;R26-iDTR (Cre+) mice (hereafter referred to as Wnt1-iDTR). R26-iDTR mice (Cre-) were used as control.
Pan W., Rahman A.A., Stavely R., Bhave S., Guyer R., Omer M., Picard N., Goldstein A.M, & Hotta R. (2022). Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy. Stem Cells Translational Medicine, 11(12), 1232-1244.
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4

Generating Conditional Six1 Knockout Mice

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The Six1 knockout homozygous (Six1−/−) and Six1 conditional knockout (Six1f/f) mouse models were generated using the CRISPR/Cas9 system on a C57BL/6J mouse background by GemPharmatech Co., Ltd (Nanjing, China). The mouse strain creation strategy involved the knockout of exon1-2 of the Six1-201 (ENSMUST00000050029.7) transcript region. Wnt1-Cre mice were obtained from the Jackson Laboratory (Bar Harbor, ME, United States). Six1f/f mice were crossed with Six1f/+; Wnt1-cre mice to generate Six1f/f; Wnt1-Cre embryos. (Supplementary Figure 1; Supplementary Table S1).
Embryos were obtained for subsequent experiments at E18.5, E16.5, and E14.5 days. The day of the appearance of a vaginal plug was defined as E0.5, and the embryos were obtained at 12:30 on each day in question. All mice were maintained and used in experiments according to the guidelines approved by the Institutional Animal Care and Use Committee (IACUC) of the Shanghai Ninth People’s Hospital affiliated to Shanghai Jiao Tong University School of Medicine.
Luo S., Liu Z., Bian Q, & Wang X. (2023). Ectomesenchymal Six1 controls mandibular skeleton formation. Frontiers in Genetics, 14, 1082911.
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5

Conditional Diphtheria Toxin Ablation

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All animal protocols were approved by the Institutional Animal Care and Use Committee at Massachusetts General Hospital (Protocols #2009N000239 and #2013N000115). All methods were carried out in accordance with relevant guidelines and regulations. The reporting in the manuscript follows the recommendations in the ARRIVE guidelines.
The following mouse lines were obtained from Jackson Laboratory (Bar Harbour, ME, USA): Wnt1::Cre mice (Stock #009107) were crossed with R26-iDTR mice (Stock #007900) to obtain Wnt1::Cre; R26-iDTR (Wnt1-iDTR) mice. R26-iDTR mice were used as controls. All mice were used at 2–3 months of age and included both sexes.
Pan W., Rahman A.A., Ohkura T., Stavely R., Ohishi K., Han C.Y., Leavitt A., Kashiwagi A., Burns A.J., Goldstein A.M, & Hotta R. (2024). Autologous cell transplantation for treatment of colorectal aganglionosis in mice. Nature Communications, 15, 2479.
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6

Wnt1-Cre x Hdac4fl/fl Murine Embryo Harvesting

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Wnt1-Cre mice were obtained from the Jackson Laboratory and crossed with Hdac4fl/fl mice. In all timed pregnancies, the day of the appearance of a vaginal plug was defined as E0.5. P0 pups and embryos were collected for serial examination. For embryo harvesting, pregnant female mice were sacrificed by CO2 intoxication. The gravid uterus was dissected out and suspended in cold PBS. The embryos were harvested after amnionectomy and removal of the placenta. Embryos at the E12.5, E14.5, E15.5, and E16.5 stages (12:00 h of the day when the vaginal plug was detected was counted as E0.5) and P0 pups were used for subsequent experiments. All animal experiments were approved by the Animal Experimental Ethical Inspection of the Shanghai Ninth People’s Hospital affiliated to Shanghai Jiao Tong University, School of Medicine.
Ha N., Sun J., Bian Q., Wu D, & Wang X. (2022). Hdac4 Regulates the Proliferation of Neural Crest-Derived Osteoblasts During Murine Craniofacial Development. Frontiers in Physiology, 13, 819619.
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7

Genotyping Transgenic Mouse Lines

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Arl13b+/− mice were kindly provided by E. Anton (UNC Neuroscience Center) and were previously described (Caspary et al., 2007 (link)). Genotyping was performed using specific primers to identify the point mutation.
Six3Cre/+ mice were previously described (Furuta et al., 2000 (link)). Primers spanning the Cre and the Six3 sequences were used for PCR genotyping.
Wnt1Cre/+ mice were purchased from Jackson Laboratory (Stock No:009107). Primers spanning the Cre and the Wnt1 sequence were used for PCR genotyping.
Gli2Lacz/+ mice were kindly provided by Alexandra Joyner (Memorial Sloan Kettering) (Bai and Joyner, 2001 (link)). Two set of primers were used for PCR genotyping. Primers spanning the LacZ and the endogenous Gli2 sequences and primers to detect WT Gli2 allele were used.
Arl13bloxP/loxP mice were kindly provided by S. Zakharenko (St. Jude Children's Research Hospital) (Su et al., 2012 (link)). PCR genotyping uses primers that distinguish the wild type and floxed Arl13b alleles.
Embryonic stages were assessed from the day of the vaginal plug that was designated as E0.5. All experimental procedures involving animals in this study were approved by IACUC and Northwestern University.
Fiore L., Takata N., Acosta S., Ma W., Pandit T., Oxendine M, & Oliver G. (2020). Optic vesicle morphogenesis requires primary cilia. Developmental biology, 462(2), 119-128.
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8

Generating Wnt1-Cre; Elp1 Conditional Knockout Embryos

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The generation of Wnt1-Cre; Elp1LoxP/LoxP CKO embryos has been previously described (George et al., 2013 (link)). Wnt1-Cre mice were purchased from the Jackson Laboratory (stock number 003829) and all strains were maintained on a C57BL/6 J background. Wnt1-Cre; Elp1LoxP/LoxP embryos were used as experimental and both Wnt1-Cre; Elp1+/LoxP and Elp1+/LoxP littermates were used as controls. All genotyping was performed via routine PCR. Elp1 CKO and wild-type alleles were distinguished using the following primers: forward (F), 5′-GCACCTTCACTCCTCAGCAT-3′ and reverse (R), 5′-AGTAGGGCCAGGAGAGAACC-3′. The presence of the Wnt1-Cre allele was detected using the following primers: F, 5′-GCCAATCTATCTGTGACGGC-3′ and R, 5′-CCTCTATCGAACAAGCATGCG-3′. All experiments were performed according to the National Institutes of Health Guide for Care and Use of Laboratory Animals and protocols were approved by the Montana State University Institutional Animal Care and Use Committee.
Cameron B., Lehrmann E., Chih T., Walters J., Buksch R., Snyder S., Goffena J., Lefcort F., Becker K.G, & George L. (2021). Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway. Biology Open, 10(9), bio058979.
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9

Genetic Mouse Models for Enteric Neuroscience

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This study was conducted in accordance with the protocols reviewed and approved by the Institutional Animal Care and Use Committee at Massachusetts General Hospital (Protocol #2009N000239). All methods were carried out in accordance with relevant guidelines and regulations. The reporting in the manuscript follows the recommendations in the ARRIVE guidelines.
Wnt1::Cre mice (Stock #003829 and Stock #009107), R26R-tdT reporter mice (Stock #007914), and R26R-ChR2tdT reporter mice (Stock #012567) were purchased from Jackson Laboratory (Bar Harbor, ME, USA). Wnt1::Cre mice were crossed with R26R-tdT and R26R-ChR2tdT reporter mice to generate Wnt1::Cre;R26-tdT (annotated as Wnt1-tdT) and Wnt1::Cre;R26-ChR2tdT (annotated as Wnt1-ChR2) mice, respectively.
We also generated Plp1-GFP;Wnt1-tdT mice [22 (link)] in which enteric glial cells express GFP and neural crest-derived ENS expresses tdTomato by crossing Plp1GFP;Wnt1::Cre mice with R26R-tdT mice. Plp1GFP mice [23 (link)] were kindly gifted by Dr. Wendy Macklin, University of Colorado, Denver. Heterozygote nNOS mice (Stock #002986) were purchased from Jackson Laboratory and bred to obtain homozygote nNOS knockout mice (nNOS−/−).
Hotta R., Rahman A., Bhave S., Stavely R., Pan W., Srinivasan S., de Couto G., Rodriguez-Borlado L., Myers R., Burns A.J, & Goldstein A.M. (2023). Transplanted ENSCs form functional connections with intestinal smooth muscle and restore colonic motility in nNOS-deficient mice. Stem Cell Research & Therapy, 14, 232.
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10

Transgenic Mice for Craniofacial Studies

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All animal procedures were approved by and followed the guidelines provided by the Texas A&M College of Dentistry IACUC committee and ARRIVE. Wwtr1tm1Hmc Yap1tm1Hmc/WranJ mice were obtained from the Jackson lab (JAX stock #030532) [21 (link)] and crossed with Wnt1-Cre mice, also from the Jackson lab. These mice were bred according to the Texas A&M IACUC protocol and then genotyped following the genotyping protocol associated with each strain developed by the Jackson lab. Taz knockouts with Yap heterozygous genotypes positive for Wnt1-Cre were considered as experimental knockout (K/O) models (hereafter mentioned as Wnt1Cre/YAP/TAZ) while C57 BL6/J mice were used as true control wild type (WT). The Wnt1-Cre mice successfully crossed with the TAZ/YAP double-floxed mice (Wnt1cre × TAZflYAPfl) were used for further studies. All mice were sacrificed as per the guidelines of the Texas A&M College of Dentistry IACUC committee.
Pandya M., Gopinathan G., Tillberg C., Wang J., Luan X, & Diekwisch T.G. (2022). The Hippo Pathway Effectors YAP/TAZ Are Essential for Mineralized Tissue Homeostasis in the Alveolar Bone/Periodontal Complex. Journal of Developmental Biology, 10(1), 14.
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