The pre-dawn leaf water potential of young fully expanded leaves on primary branches was measured (04:00–05:00h WST) in a pressure chamber (Soil Moisture Equipment Corp., Santa Barbara, CA, USA). On similar leaves to those used for leaf water potential, measurements of gas exchange were carried out between 10:30h and 12:00h WST using a LICOR-6400 with a red/blue LED light source (LI-COR, Lincoln, NE, USA). Photosynthetic photon flux density at the leaf surface was set at 1500 μmol m−2 s−1, block temperature at 25 °C, flow rate at 500 μmol s−1, and ambient CO2 concentration of the incoming gas stream at 380 μmol mol−1 as in Pang et al. (2011) .
Red blue led light source
Manufactured by LI COR
Sourced in United States
The Red/Blue LED Light Source is a versatile laboratory equipment designed to provide stable and uniform illumination for a variety of applications. It features high-intensity red and blue LED lights that can be controlled independently or simultaneously. The device is capable of delivering consistent and reproducible light output, making it a reliable tool for various experimental setups.
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Lab products found in correlation
3 protocols using red blue led light source
1
Leaf Water Potential and Gas Exchange
2
Quantifying Photosynthesis and Transpiration
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Net photosynthetic (Pn) and transpiration (E) rates were measured at the end of the drought treatment in six plants per treatment combination, and their weights added to the remaining leaves for fresh and dry weight determinations (n = 6) using the LI-6400XT portable open-flow photosynthesis system equipped with a red/blue LED light source (LI-COR, Inc., Lincoln, NE, USA). The PPFD was set at 400 μmol∙m−2∙s−1, leaf temperature at 28 °C, and the reference CO2 concentration was maintained at 400 μmol∙mol−1 using the 6400-01 CO2 mixer. All measurements were carried out between 4 and 8 h after the onset of the photoperiod on the upper fully expanded leaves. Leaf areas were calculated following computer scanning using the Sigmascan Pro 5.0 computer software (Systat Software, San Jose, CA, USA).
3
Diurnal Photosynthesis and Stomatal Dynamics
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Net photosynthetic rate and stomatal conductance of P. bifurcatum, A. angustifolia, and P. costata were measured using a portable mini cuvette system, the LI-6400 infrared gas analyzer with a red/blue LED light source (LI-COR, Lincoln, NE, USA). A single leaf was clamped in the cuvette, ensuring that it received full light (500 μmol m−2 s−1) within the growth chamber. Environmental conditions used for net photosynthetic rate and stomatal conductance measurement were consistent with those at which samples were harvested for metabolome, proteome, and transcriptome sequencing. Net photosynthetic rate and stomatal conductance data were collected every minute, and gas flow through the cuvette was maintained at around 450 mL min−1 to avoid water condensation inside the cuvette. Each leaf was maintained inside the cuvette for 24 h to obtain a complete 24-h gas exchange profile.
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