The cell suspension was prepared from collagenase D-treated and dissociated spleen, skin-draining lymph nodes, perigonadal white adipose tissue, lungs, and liver of wild-type 8-week-old male and female C57BL/6J mice (Jackson Labs).63 (link) The cells were stained with Aqua Live/Dead kit followed by staining with 10 mg/mL of Perfringolysin O (PFO) from Clostridium perfringens (Cusabio # CSB-EP314820CMB) in PBS at +25°C for 30 min, washed 3 times, and stained with an antibody cocktail (CD45 BUV563 clone 30-F11, I-A/I-E BUV496 clone M5/114.15.2 from BD, CD11c PE-Cy7 clone N418, CD3e AF488 clone 145-2C11, CD19 FITC clone 1D3/CD19, NK1.1 AF488 clone PK136, TER-119 AF488 clone TER-119, F4/80 AF488 clone BM8 from BioLegend, anti-Perfringolysin O rabbit antibody [Abcam # ab225685] and Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody AF647 [Thermofisher # A-21245]) and Fc-block for 30 min on ice. The cells were analyzed by flow cytometry using FACSymphony A3 Cell Analyzer and FlowJo software.
Cd11c pe cy7 clone n418
Manufactured by BD
CD11c PE-Cy7 clone N418 is a fluorochrome-conjugated antibody that binds to the CD11c cell surface antigen. CD11c is expressed on the surface of myeloid dendritic cells, macrophages, and some natural killer cells. This antibody can be used for the identification and analysis of cells expressing CD11c in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
A 21245, by Thermo Fisher Scientific (1 mentions)
F4 80 af488 clone bm8, by BioLegend (1 mentions)
Bsa stain buffer, by BD (1 mentions)
Cxcr3 apc, by BD (1 mentions)
Fixable viability stain, by BD (1 mentions)
Ly6g percp cy5.5 clone 1a8, by BD (1 mentions)
Cd45 apc cy7 clone 30 f11, by BD (1 mentions)
Fortessa flow cytometer, by BD (1 mentions)
Pd l1 bv421, by BD (1 mentions)
Ly6c bv421, by BD (1 mentions)
2 protocols using cd11c pe cy7 clone n418
1
Multiorgan Immune Cell Profiling
2
Multiparametric Flow Cytometry Panel
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Cells were resuspended in PBS and stained on ice for 30 minutes in the dark with a fixable viability stain (BD Bioscience). Then, cells were resuspended into the stain buffer (BSA) (BD bioscience) and stained on ice for 30 minutes with various combinations of directly fluorochrome-conjugated antibodies. Antibodies used for flow cytometry were from BD Biosciences, Biolegend, or ThermoFisher, and include CD45 APC-Cy7 (clone 30-F11), CD8a BUV737 (clone 53-6.7), TCRb PE (clone H57-597), CD4 BUV395 (clone RM4-5), CD25 BV711 (clone PC61), CD11b AF700 (clone M1/70), Ly6G PercpCy5.5 (clone 1A8), CD11c PeCy7 (clone N418), Nkp46 FITC (clone 29A1.4), F4/80 PE-CF594 (clone T45-2342) Ly6C BV421 (clone AL-21), CXCR3 APC (clone CXCR3-173), Class I MHC FITC (clone 34-1-25) and PD-L1 BV421 (clone MIH5). For all samples, acquisition was performed on Fortessa flow cytometer (BD). Data were analyzed using FlowJo software (Tree Star).
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